Effects of Platelet-Rich Fibrin/Collagen Membrane on Sciatic Nerve Regeneration

Abstract

Alternative treatment approaches to improve the regeneration ability of damaged peripheral nerves are currently under investigation. The aim of the current study was to evaluate the effects of leucocyte/platelet-rich fibrin (L-PRF) with or without a collagen membrane as a supporter on crushed sciatic nerve healing in a rat model. Recovery of motor function and electrophysiologic measurements were evaluated at 4 weeks postoperatively. The whole number of myelinated axons, peripheral nerve axon density, average nerve fiber diameter (μm), and G-ratio were analyzed and compered among the groups. Functional, electrophysiological, and histological evaluations showed no significant difference among the groups with the exception of the L-PRF with collagen membrane groups that showed relatively positive effects on the functional and histological nerve recovery. In addition, the collagen membrane with L-PRF can be effect in nerve regeneration.

The Effect of Leukocyte- and Platelet-Rich Fibrin on Central and Peripheral Nervous System Neurons—Implications for Biomaterial Applicability

“…Roth et al provided the first suggestive evidence that L-PRF treatment had a similar effect on the functional outcome after traumatic nerve injury as an autograft, which is the gold standard therapy in the clinic [77]. More recent publications by Vares et al [78] and Neves Atti et al [79] also suggest positive effects of L-PRF treatment in a rat model for peripheral nerve injury. However, care should be taken and additional preclinical research is necessary to understand the applicability of L-PRF for improving nerve tissue regeneration in the clinic.…”

Efficacy and safety of autologous platelet‐rich fibrin for the treatment of infertility with intrauterine adhesions

“…PRF is light yellow and gelatinous at room temperature and has certain flexibility and profiling properties. Due to the advantages of economy, slow‐release, solid properties, non‐immunogenicity, and natural degradation, PRF has been widely used in clinical applications, and the exploration of its repair potential has been studied in various fields, 30–32 such as maxillofacial bone regeneration, 33 nerve regeneration, 34 so on. However, PRF's effect on the endometrium of patients with IUAs has not been clarified.…”

Recent Achievements in the Development of Biomaterials Improved with Platelet Concentrates for Soft and Hard Tissue Engineering Applications

“…The PRP was also found to be an effective strategy for repairing peripheral nerve injury [51]. The PRF showed good in vivo regeneration of the crushed sciatic nerve healing in a rat model [52]. The use of CGFs, on the other hand, positively affects neural cell differentiation by regulating the expression of neuronal markers [53].…”