Effects of plasmids on chromosome metabolism in bacteria

Chernin, Leonid; Mikoyan, Vladimir S.

doi:10.1016/0147-619x(81)90059-7

Cited by 15 publications

(6 citation statements)

References 103 publications

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“…Thus, the LexA protein appears to function not only as the repressor of a large set of chromosomal genes-recA, lexA, uvrA, uvrB, sulA, himA, dinA, dinB, MucA-_ FIG. 5. Repression of the synthesis of the mucB-lacZ hybrid protein and the MucA protein by the LexA protein in maxicelis.…”

Section: Discussionmentioning

confidence: 99%

“…Plasmid pKM101 is a 34.5-kilobase (kb) Nincompatability-group plasmid that was derived from the clinically isolated plasmid R46 by a series of in vivo manipulations (16,22). It is one of a number of plasmids that increase the susceptibility of Escherichia coli and Salmonella typhimurium to mutagenesis by a variety of agents, including UV radiation, and also increase the resistance of these bacteria to killing by UV radiation (5). The capacity of pKM101 to enhance mutagenesis is closely associated with its ability to enhance recovery from UV damage since single mutations have been isolated which affect both processes (27,33).…”

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confidence: 99%

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The muc genes of pKM101 are induced by DNA damage

Elledge¹,

Walker²

1983

J Bacteriol

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A gene fusion was constructed in vitro that resulted in the synthesis of a hybrid protein consisting of the amino-terminal segment of the MucB protein of the mutagenesis-enhancing plasmid pKM101 joined to an enzymatically active carboxy-terminal segment of the beta-galactosidase protein. In strains bearing this fusion, beta-galactosidase activity was induced by UV radiation and other DNA-damaging agents. A genetic analysis of the regulation of expression of the phi (mucB'-lacZ') fusion was consistent with the LexA protein acting as the direct repressor of the mucB gene. Examination of the expression of the mucA and phi (mucB'-lacZ') gene products in maxicells in the presence and absence of a high-copy-number plasmid carrying the lexA+ gene demonstrated that lexA regulated both the mucA and mucB genes, thus supporting our conclusion that the two genes are organized in an operon with the mucA gene transcribed first. An analysis of the effects of the recA430(lexB30) mutation on muc expression led to the discovery of the differential ability of the recA430 gene product to induce expression of a dinB::Mu d1(Ap lac) fusion located on the chromosome and the same phi (dinB'-lacZ+) fusion cloned into plasmid pBR322. Models to account for the role of the recA430 allele on the expression of damage-inducible genes and on mutagenesis are discussed.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

The muc genes of pKM101 are induced by DNA damage

Elledge¹,

Walker²

1983

J Bacteriol

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“…A number of naturally occurring plasmids (2,48,236), for example R46 (83,247), R205 (212), TP110 (81), and Col I (144,145), have the ability to make both E. coli and S. typhimurium more resistant to killing by UV and a number of chemicals and more susceptible to mutagenesis by these agents. As discussed in more detail below, it has been shown recently that at least some of these naturally occurring plasmids carry analogs of the E. coli umuD and umuC genes (77,265,324,334,347,357,360).…”

Section: Umud and Umuc Analogs On Naturally Occurring Plasmidsmentioning

confidence: 99%

Mutagenesis and inducible responses to deoxyribonucleic acid damage in Escherichia coli.

Walker¹

1984

Microbiological Reviews

1,034

410

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“…Of the plasmids which affect post-irradiation survival in E. coli, most reports have centred on plasmids which enhance the survival of the host (Chernin & Miroyan, 1981). One of the best-studied plasmids of this type, pKM101, has been shown to encode an analogue of umuC, a gene involved directly in error-prone repair (Walker & Dobson, 1979).…”

Section: Introductionmentioning

confidence: 99%

The Effect of Plasmid R391 and Other IncJ Plasmids on the Survival of Escherichia coli After UV Irradiation

Pembroke

Stevens

1984

Microbiology

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~~The presence of the IncJ plasmids R391, R997, R705, R706, R748 and R749 was shown to sensitize Escherichia coli AB1157 and both its uvrA and ZexA derivatives to UV irradiation. No alteration in post-irradiation survival was observed in a recA mutant containing these plasmids, compared with the non-plasmid-containing recA strain. Analysis of recombination frequency in Hfr crosses to r e d + cells containing plasmid R391 indicated a reduction in recombination frequency compared with that obtained in similar crosses to a non-plasmid-containing strain. This effect was not due to plasmid-encoded restriction or entry exclusion systems and therefore must be considered as a real block in recombination. When cells containing plasmid R391 were irradiated and allowed to photoreactivate, an increase in survival was observed which was comparable to that observed in the non-plasmid-containing derivative. This indicated that postirradiation processing of UV-induced damage, or lack of such processing, by mechanisms other than photoreactivation was responsible for the UV sensitivity associated with plasmid R391.

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Effects of plasmids on chromosome metabolism in bacteria

Cited by 15 publications

References 103 publications

The muc genes of pKM101 are induced by DNA damage

The muc genes of pKM101 are induced by DNA damage

Mutagenesis and inducible responses to deoxyribonucleic acid damage in Escherichia coli.

The Effect of Plasmid R391 and Other IncJ Plasmids on the Survival of Escherichia coli After UV Irradiation

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