Effects of Plant Stress Signal Molecules on the Production of Wilforgine in an Endophytic Actinomycete Isolated from Tripterygium wilfordii Hook.f.

Abstract

The endophytic actinomycete F4-20 was isolated from Tripterygium wilfordii Hook.f. and was confirmed to produce wilforgine, a secondary metabolite discovered in its host. F4-20 showed a close phylogenetic relationship to Streptomyces species. To seek elicitors that may enhance the production of wilforgine in F4-20, four plant stress molecules were applied to the in vitro liquid cultures. Results showed that methyl jasmonate (MeJA), salicylic acid (SA), and hydrogen peroxide (H2O2) inhibited bacterial growth, whereas glutathione (GSH) treatment significantly increased bacterial growth. The wilforgine contents in the mycelia of F4-20 were reduced by MeJA and GSH but were induced by SA and H2O2. When added in the end of the culture period (7 day), 1 mM SA and 5 mM H2O2 resulted in 69.35 ± 1.71 and 71.80 ± 3.35 µg/g DW of wilforgine production, 1.55 and 1.60 fold to that of control (44.83 ± 1.35 µg/g DW), respectively. Though this improved production was about 6.5 times lower than that of the natural root (454.00 µg/g dry root bark), it provided an alternative method for the production of valuable plant secondary metabolites.

Community Structures and Antifungal Activity of Root-Associated Endophytic Actinobacteria of Healthy and Diseased Soybean

“…Recently, endophytic actinobacteria have attracted significant interest for their capacity to produce abundant bioactive metabolites, which may contribute to their host plants by promoting growth and health [21,22]. A vast majority of endophytic actinobacteria have been isolated from a variety of plants including various crop plants, medicinal plants, and different woody tree species [23,24,25,26,27,28]. Further, recent cultivation-independent analysis using 16S rRNA gene-based methods revealed that actinobacteria can be specifically enriched in plant roots, and are more abundant in diseased plants than in healthy plants, which may provide probiotic functions for the host plants [29,30,31].…”

Community Composition and Metabolic Potential of Endophytic Actinobacteria From Coastal Salt Marsh Plants in Jiangsu, China

“…Actinobacterial species are the main producers of active microbial natural products, are ubiquitously distributed among environments, and have been widely used in industrial and agricultural applications (Bèrdy, 2005; Qin et al, 2016). Recently, endophytic actinobacteria have particularly attracted significant attention, with increasing documentation of isolates from a wide range of plants, including various crop plants like wheat, rice, banana, apple, and tea plants, in addition to medicinal plants such as Artemisia annua , Tripterygium wilfordii Hook, Glycyrrhiza inflata , and Jatropha curcas (Conn and Franco, 2003; Tian et al, 2007; Qin et al, 2011, 2015b; Li et al, 2012; Miao et al, 2015; Álvarez-Pérez et al, 2017; Wei et al, 2018; Zhao et al, 2018). Streptomyces , Micromonospora , Micrococcus , Pseudonocardia , and Microbacterium are the most predominant endophytic actinobacterial genera cultivated, and many novel species from these genera have been identified in diverse host plants (Kaewkla and Franco, 2013; Golinska et al, 2015; Dinesh et al, 2017).…”

Regulating Gut Microbiome: Therapeutic Strategy for Rheumatoid Arthritis During Pregnancy and Lactation

“…Xiao M et al found that the diversity of gut microbiome increased in collageninduced arthritis mice treated with Paederia scandens (Chinese herbal medicine) (Xiao et al, 2018). In addition, the therapeutic effect of another Chinese herbal medicine, Tripterygium wilfordii (Chinese herbal medicine), used to treat RA has also been shown to be related to gut microbiome (Miao et al, 2015). After receiving the treatment of Tripterygium wilfordii, the abundance of Prevotella intermedia in the intestine of RA patients was increased (Zhang et al, 2015).…”