Effects of plant growth regulators and photoperiod on in vitro microtuberization of potato (Solanum tuberosum L.)

Abstract

In vitro microtuber production of potato (Solanum tuberosum L.) cvs. Sante and Savalan were studied on solid Murashige and Skoog (MS) basal medium applying different plant growth regulators 2,4dichlorophenoxyacetic acid and benzylamino purine (2,4-D and BAP) and photoperiods. Cultures were exposed to 16, 8 and 16 h+utter darkness photoperiodic regimes. The experimental design, complete randomized with three replications was applied. The results indicate that the effect of cultivar, hormone and photoperiod significantly had influence on whole traits. Sante cultivar had higher productivity than Savalan for whole measured traits. The results of mean comparison for photoperiod show that highest productivity for whole traits is gained by this treatment 16 h photoperiod+ utter darkness (P 3). In this experiment by using the combination of 2,4-D (2.26 µM) and BAP (22.19 µM), microtubers number, diameter and weight was increased. In this study, the highest number for microtuber in Sante cultivar with hormone 2,4-D and photoperiod P 3 is 9.47 which this high number for Savalan cultivar is gained by using the combination of two plant growth regulators and same photoperiod.

Yield and seed productivity of pre-basic seed material depending potatoes on growing technology elements

“…äîâåäåíî, ùî çàñòîñóâàííÿ ð³ñòðåãóëþþ÷èõ ðå-÷îâèí ñïðèÿëî ï³äâèùåííþ ñò³éêîñò³ êàðòîï-ë³ äî ðàííüîãî òà ï³çíüîãî ïðîÿâó ô³òîôòîðîçó, çðîñòàííþ óðîaeàéíîñò³ áóëüá òà ïîêðàùåííþ ôðàêö³éíîãî ñêëàäó âðîaeàþ [4]. ×èñëåííèìè äîñë³äaeåííÿìè âñòàíîâëåíî ïîçèòèâíèé âïëèâ çàñòîñóâàííÿ ð³ñòðåãóëþ-þ÷èõ ðå÷îâèí íà ïîêàçíèêè ðîñòó òà ðîçâèòêó ðîñëèí êàðòîïë³ in vitro çà ì³êðîêëîíàëüíîãî ðîçìíîaeåííÿ [5][6][7][8][9][10]. Çà âèçíà÷åííÿ âïëèâó ð³ñòðåãóëÿòîð³â íà ïðîäóêòèâí³ñòü îçäîðîâëåíî¿ êàðòîïë³ íà ðîçñàäí³é êóëüòóð³ âñòàíîâëåíî, ùî ïîçàêîðåíåâå ï³äaeèâëåííÿ ã³áåðåë³íîì À 3 â äîç³ 20 ã/ãà ï³äâèùóâàëî âðîaeàéí³ñòü êàðòîïë³ íà 19-26%, çá³ëüøóâàëî êîåô³ö³ºíò ðîçìíîaeåííÿ ðîñëèí íà 18-22%, âèõ³ä áóëüá ç îäèíèö³ ïëîù³ íà 17-18%.…”