The present investigation entitled “Effect of plant growth regulators Gibberellic Acid (GA3) and Salicylic Acid (SA) on the growth and yield of carnation under naturally ventilated polyhouse”, was carried out during November, 2021 to April 2022 in, Naturally Ventilated Polyhouse, Department of Horticulture, Naini Agricultural Institute, SHUATS, it was concluded that the plant growth regulators treatments rendered their significant effect on almost all the growth, flowering and yield characters as well as quality of carnation. The treatment T3 i.e. application of GA3 @ 200 ppm was found superior in terms of Plant height (102.27cm), number of leaves (203.67), number of shoots (7.67), internodal length (7.73cm), number of internodes per shoot (17), bud length (10cm), bud diameter (2.87cm), flower diameter (6.80cm), flower length (5.67cm), flower stalk length (96.93cm), vase life (10.33), number of cut flower stalks per plant (7.00) and number of cut flower stalks per square meter (175). However, plants treated with SA @ 120ppm took minimum days for bud initiation (112.67 days) and the days taken to bud opening (24.67). Among the different treatments the highest Gross return (Rs/200m2) (7,00,000), Net return (441565), benefit cost ratio (2.70) was obtainedunder the use of GA3 @ 200 ppm (T3).