Effects of plant essential oils on growth and virulence factors of Erwinia amylovora

Akhlaghi, Mahdi; Tarighi, Saeed; Taheri, Parissa

doi:10.1007/s42161-019-00446-9

Cited by 25 publications

(14 citation statements)

References 42 publications

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“…The effect of polyphenols on amylovoran production has not been investigated yet, but our results suggest that this is one of the main pathways involved in MOEs’ activity: In this study, we demonstrate that MOEs negatively affect EA virulence in vitro and in planta, as it was confirmed that amylovoran is needed for pathogenicity on immature pear fruits and pear plants [ 45 , 46 ].…”

Section: Discussionsupporting

confidence: 56%

Control of Erwinia amylovora Growth by Moringa oleifera Leaf Extracts: In Vitro and in Planta Effects

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et al. 2022

Plants

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Erwinia amylovora (EA) is a phytopathogenic bacterium, the causative agent of bacterial fire blight, a disease that affects Rosaceaes. In order to replace antibiotics and copper, the antimicrobial activity of three extracts of Moringa oleifera Lam., methanolic (MeOH-MOE), hydroalcoholic (HA-MOE) and hydroalcoholic with maltodextrins (HAMD-MOE), was tested on eleven strains of EA isolated from apple trees by the Emilia-Romagna Phytosanitary Department. MIC and MBC have been evaluated; biofilm formation, swarming motility and amylovoran production were performed with the crystalviolet, soft-agar assay and the amylovoran method. All extracts demonstrated bacteriostatic activity at a concentration of 1 mg/mL, resulting in a 80% reduction in biofilm formation. HAMD-MOE, MeOH-MOE and HA-MOE caused an inhibition of motility of 60%, 65% and 30% after 6 days and a decrease in amylovoran synthesis of 84%, 63% and 93%, respectively. In planta results showed how the compounds were able to inhibit EA virulence on apple trees, mainly if they were applied as a preventive treatment, although the treatment showed a significant reduction in fire blight symptoms progression. The antibacterial activity of the extracts is mainly due to the high concentration of polyphenolic compounds detected in the extracts that was able to alter the permeability of bacterial membrane, resulting in slowing the synthesis of ATP and consequently of all ATP-dependent functions, such as motility and less selectivity towards harmful compounds, which can, thus, enter the cytoplasm and inhibit enzymes involved in replication and quorum sensing. The efficacy, eco-compatibility and low cost make such extracts a potential tool for the control of bacterial fire blight.

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Section: Discussionsupporting

confidence: 56%

Control of Erwinia amylovora Growth by Moringa oleifera Leaf Extracts: In Vitro and in Planta Effects

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Macchi

Caproni

et al. 2022

Plants

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“…Stocks of the bacterial strains were conserved at −80 The MIC is defined as the lowest concentration of a chemical molecule that blocks visible bacterial growth after an overnight incubation. In order to determine the MIC, the microdilution method was used, as described by Akhlaghi et al [23]. Xcc was cultured in LB broth overnight at 25 • C, 160 rpm.…”

Section: Campestris Pv Campestris Strains and Culture Conditionsmentioning

confidence: 99%

Effects of Moringa oleifera Leaf Extracts on Xanthomonas campestris pv. campestris

et al. 2021

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Xanthomonas campestris pv. campestris (Xcc) is a Gram-negative bacterium belonging to the Xanthomonodaceae family, causing black rot in crucifers. To control this pathogen, the study investigated the effect of different leaves extracts of Moringa oleifera Lam., a tropical plant, well known for its food properties and with countless applications in many different fields, from nutraceutical (hypoglycemic) to the cosmetic (sunscreen) properties. Nevertheless, several studies pointed to its antibacterial action against both Gram-negative and Gram-positive bacteria. Many bioactive compounds, including flavonoids, phenolic acids, alkaloids, isothiocyanates, tannins and saponins, contained in these extracts, are responsible for its countless activities. The analyses carried out in this study show that the methanolic, hydroalcoholic and hydroalcoholic maltodextrin extracts have both bacteriostatic and bactericidal effects at concentrations of 0.5, 0.5 and 0.1 mg/mL respectively. In particular, the study shows how all extracts can alter membrane permeability, to adversely affect swarming motility, and to alter biofilm formation in Xcc. The in planta experiments showed a reduction of the necrosis area in the infected radishes, although the ability of the extracts to be absorbed by root systems is yet to be understood, in order to reach the target point.

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“…From an in vivo study, EOs of Apium graveolens (celery seed) and Curcuma longa (turmeric) demonstrated the greatest reduction in the impact of E. amylovora virulence factors. They reduced disease progression of E. amylovora on immature pear fruit by 41.71 and 30.17%, and disease progression in pear seedling shoots by 26.9 and 16.7%, respectively ( 144 ).…”

Section: Properties Of Eos As Broad-spectrum Anti-microbial Agentsmentioning

confidence: 99%

Biocontrol Potential of Essential Oils in Organic Horticulture Systems: From Farm to Fork

et al. 2022

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In recent decades, increasing attention has been paid to food safety and organic horticulture. Thus, people are looking for natural products to manage plant diseases, pests, and weeds. Essential oils (EOs) or EO-based products are potentially promising candidates for biocontrol agents due to their safe, bioactive, biodegradable, ecologically, and economically viable properties. Born of necessity or commercial interest to satisfy market demand for natural products, this emerging technology is highly anticipated, but its application has been limited without the benefit of a thorough analysis of the scientific evidence on efficacy, scope, and mechanism of action. This review covers the uses of EOs as broad-spectrum biocontrol agents in both preharvest and postharvest systems. The known functions of EOs in suppressing fungi, bacteria, viruses, pests, and weeds are briefly summarized. Related results and possible modes of action from recent research are listed. The weaknesses of applying EOs are also discussed, such as high volatility and low stability, low water solubility, strong influence on organoleptic properties, and phytotoxic effects. Therefore, EO formulations and methods of incorporation to enhance the strengths and compensate for the shortages are outlined. This review also concludes with research directions needed to better understand and fully evaluate EOs and provides an outlook on the prospects for future applications of EOs in organic horticulture production.

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Effects of plant essential oils on growth and virulence factors of Erwinia amylovora

Cited by 25 publications

References 42 publications

Control of Erwinia amylovora Growth by Moringa oleifera Leaf Extracts: In Vitro and in Planta Effects

Control of Erwinia amylovora Growth by Moringa oleifera Leaf Extracts: In Vitro and in Planta Effects

Effects of Moringa oleifera Leaf Extracts on Xanthomonas campestris pv. campestris

Biocontrol Potential of Essential Oils in Organic Horticulture Systems: From Farm to Fork

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