Effects of Phytoestrogen Extracts Isolated from Rye, Green and Yellow Pea Seeds on Hormone Production and Proliferation of Trophoblast Tumor Cells Jeg3

Abstract

Background: Phytoestrogens are a diverse group of non-steroidal plant compounds. Because they have chemical structures similar to estrogens they are able to bind on estrogen receptors in humans. Objectives: In this study, we tested the effects of crude phytoestrogen extracts from rye (Secale cereale), green pea (Pisum sativum) and yellow pea seeds (Pisum sativum cv.) on cell proliferation and the production of progesterone in trophoblast tumor cells of the cell line Jeg3. Methods: Isoflavone extracts from green and yellow pea seeds and lignan extracts from rye seeds were obtained, using different extraction methods. Isolated extracts were incubated in different concentrations with trophoblast tumor cells. Untreated cells were used as controls. At designated times, aliquots were removed and tested for estradiol and progesterone production. In addition, we tested the effects of the phytoestrogen extracts on cell proliferation. Results: Cell proliferation is significantly inhibited by potential phytoestrogens isolated from rye, green and yellow pea seeds in trophoblast tumor cells of the cell line Jeg3. We found a correlation between the effects of proliferation and production of estradiol in isoflavone extracts from green and yellow pea seeds in Jeg3 cells. In addition, higher concentrations of isoflavones isolated from green pea seeds and lignans from rye showed also a inhibition of progesterone production whereas higher concentrations of rye lignans elevated estradiol production in Jeg3 cells. Conclusion: A useful indicator test system for potential phytoestrogens could be established. Based on the obtained results it is proposed that green and yellow pea seeds contain measurable concentrations of isoflavones and rye seeds contain lignans which can be isolated and used for special human diet programs.

miRNAs as common regulators of the transforming growth factor (TGF)-β pathway in the preeclamptic placenta and cadmium-treated trophoblasts: Links between the environment, the epigenome and preeclampsia

“…This cell line maintains characteristics of first trimester trophoblast cells (Matsuo and Strauss 1994) and has been used to assess biological effects of exposures to environmental toxicants (Bergemann et al 2003; Canettieri et al 2008; Ding et al 2011; Koc et al 2003; Matscheski et al 2006; Adebambo et al 2015; Guan et al 2013; Kummu et al 2012; Alvarez and Chakraborty 2011). Cells were grown in Dulbecco’s Eagle Minimum Essential Media with 10% FBS, sodium pyruvate, and penicillin/streptomycin at 37°C with 5% CO 2 .…”

Effects of phytoestrogens genistein and daidzein on progesterone and estrogen (estradiol) production of human term trophoblast cellsin vitro

“…Genistein in comparison to daidzein was a more sufficient inhibitor for the production of hCG [13]. In addition, our former investigation on trophoblast tumor cells with different phytoestrogens showed effects on cell proliferation and hormone production [13][14][15][16][17].…”

Effects of Phytoestrogen Extracts Isolated from Elder Flower on Hormone Production and Receptor Expression of Trophoblast Tumor Cells JEG-3 and BeWo, as well as MCF7 Breast Cancer Cells

“…For the determination of estradiol in the culture medium, a competitive enzyme immuno-assay (EIA) was applied as described previously [29]. The measurements were performed using an automated Immulite 2000 (DPC Biermann, Freiburg, Germany) hormone analyzer.…”