Effects of physical training on sarcomere lengths and muscle-tendon interface of the cervical region in an experimental model of menopause

Jacob, Carolina Dos Santos; Rocha, Lara Caetano; Neto, Jurandyr Pimentel; Watanabe, Ii-sei; Ciena, Adriano Polican

doi:10.4081/ejh.2019.3038

Cited by 16 publications

(21 citation statements)

References 31 publications

(26 reference statements)

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“…In the soleus muscle, we found greater sarcomere lengths and the higher variation in distal sarcomere (CTR). Ertbjerg and Puolanne (2017) presents variations in sarcomere length due to their location within the same myofilament, while Jacob et al (2019) presented a variety of proximal and distal sarcomeres with shorter lengths, findings that are not consistent with our results for the soleus muscle.…”

Section: Discussioncontrasting

confidence: 99%

“…In the distal muscle region, there is a complex and specialized interface, the myotendinous junction (MTJ), where the penultimate and last sarcomeres are located, denominated, respectively, the proximal and distal sarcomeres, which have been demonstrated to have plasticity in their lengths associated with experimental models of menopause and aerobic training (Jacob, Rocha, Neto, Watanabe, & Ciena, 2019; Sierra et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

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Repercussions on sarcomeres of the myotendinous junction and the myofibrillar type adaptations in response to different trainings on vertical ladder

Rocha

Neto

Sant'Ana

et al. 2020

Microscopy Res & Technique

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The myofibrillary types establish to the skeletal muscle functional and adaptive properties that influence the sarcomeric arrangement during muscle contraction and may have repercussions on an important related force transmission region of the locomotor apparatus, the myotendinous junction (MTJ). This study aimed to describe changes in myofibrillary type and sarcomeric lengths in the belly muscle and MTJ of the soleus and plantaris muscles associated with training protocols in vertical ladder. Thirty adults male Wistar rats were divided into three groups (n = 10): Control (CTR), No‐load Training (NLT), and Load Training (LT). Morphoquantitative analysis of different fibers types and sarcomere lengths were performed in distinct regions of plantaris and soleus muscles. In the plantaris muscle with both trainings, there was an increase in the cross‐sectional area (CSA) in Type I and II fibers (p < .0001) while sarcomeric lengths revealed greater lengths in the proximal and distal sarcomeres of NLT, although in the LT we found greater lengths in the belly and MTJ sarcomeres. The soleus muscle showed an increase in CSA muscle fiber only in the NLT (p < .0001) and revealed alterations in belly and MTJ sarcomere lengths with training. We concluded that plantaris muscle has an adaptive effect directly associated with training load, with hypertrophy in both trainings and sarcomere length inverse from belly and MTJ, in LT associated with increased force generation and transmission at the MTJ, although soleus muscle has a lower adaptive response to training stimuli with variation in the belly and distal sarcomere of the MTJ.

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Section: Discussioncontrasting

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Repercussions on sarcomeres of the myotendinous junction and the myofibrillar type adaptations in response to different trainings on vertical ladder

Rocha

Neto

Sant'Ana

et al. 2020

Microscopy Res & Technique

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“…Rats (n = 5) were euthanized with an anesthetic overdose (ketamine at 100 mg/kg and xylazine at 5 mg/kg). MTJ samples of the plantaris muscle (3 mm 3 ) were immersed in 0.1 M sodium cacodylate solution (pH 7.4) at 4 °C, post-fixed with 1% osmium tetroxide solution 42 , washed with 0.9% saline, and fixed with 0.5% uranyl acetate solution. Then, the samples were dehydrated in a series of alcohols and propylene oxide (2×), followed by a mixture of resin (Spurr) and propylene oxide, and then placement in pure resin for 12 h. The samples were heated at 37 °C in rectangular silicone molds filled with pure resin and kept at 60 °C.…”

Section: Methodsmentioning

confidence: 99%

Myotendinous junction adaptations to ladder-based resistance training: identification of a new telocyte niche

Neto

Rocha

Barbosa

et al. 2020

Sci Rep

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The present study shows chronic adjustments in the myotendinous junction (MTJ) in response to different ladder-based resistance training (LRT) protocols. Thirty adult male Wistar rats were divided into groups: sedentary (S), calisthenics (LRT without additional load [C]), and resistance-trained (LRT with extra weight [R]). We demonstrated longer lengths of sarcoplasmatic invaginations in the trained groups; however, evaginations were seen mainly in group R. We showed a greater thickness of sarcoplasmatic invaginations in groups C and R, in addition to greater evaginations in R. We also observed thinner basal lamina in trained groups. The support collagen layer (SCL) adjacent to the MTJ and the diameters of the transverse fibrils were larger in R. We also discovered a niche of telocytes in the MTJ with electron micrographs of the plantar muscle and with immunostaining with CD34+ in the gastrocnemius muscle near the blood vessels and pericytes. We concluded that the continuous adjustments in the MTJ ultrastructure were the result of tissue plasticity induced by LRT, which is causally related to muscle hypertrophy and, consequently, to the remodeling of the contact interface. Also, we reveal the existence of a collagen layer adjacent to MTJ and discover a new micro anatomic location of telocytes. The myotendinous junction (MTJ) consists of a highly specific anatomical region in which the sarcoplasmatic membranes connect to bundles of extracellular matrix (ECM) collagen fibers 1. Due to its functionality, MTJ represents the region with the highest transmission of force 2,3. Morphologically, MTJ presents projections that penetrate the muscle tissue, parallel and directed to the myofibrils, forming sarcoplasmatic invaginations. Currently, we know that its development is mainly influenced by the interactions between myoblasts and ECM elements 4,5. The basal lamina of muscle fiber creates a supramolecular connection structure composed of different levels of proteins from the laminin and collagen polymers located in the ECM 6. This arrangement contributes structurally to the transmission of force, adapting, remodeling, and consequently affecting the macroscopic tendon structure to different stimuli 7,8. Initially, telocytes were described as interstitial Cajal cells 9. Telocytes are interstitial cells of stromal origin with an oval shape, and a heterochromatic nucleus with moniliform projections denominated telopodes, which characterize its morphological classification according to the number of projections 9,10. They are founded in the interstitium of tissues such as the testicle 9 , smooth and cardiac muscle 11 , human tongue 10 , pancreas 12 , and liver 13. It can be identified by the electronic microscopy, immunohistochemistry, and CD34+/immunostaining 14. Recently, telocytes have been found in proximity to and engaging in possible interactions with satellite cells 10. The telocytes projections have terminals (pods) with mitochondria that form junctions with adjacent cells, and they have caveolae that perform exocytos...

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“…Author: Louisa Howard). (B) Transmission electron microscopy of the MTJ of the rat sternomastoid muscle (M = muscle side; T = Tendon side) (scale bar = 0.5 μm) adapted from Jacob et al (2019) (reproduced with permission reproduced with permission under the terms of the CC BY-NC 4.0 license. Copyright 2019, PAGEPress.).…”

Section: Main Tissues Of the Musculoskeletal Systemmentioning

confidence: 99%

Tissue Engineering for the Insertions of Tendons and Ligaments: An Overview of Electrospun Biomaterials and Structures

Sensini

Massafra

Gotti

et al. 2021

Front. Bioeng. Biotechnol.

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The musculoskeletal system is composed by hard and soft tissue. These tissues are characterized by a wide range of mechanical properties that cause a progressive transition from one to the other. These material gradients are mandatory to reduce stress concentrations at the junction site. Nature has answered to this topic developing optimized interfaces, which enable a physiological transmission of load in a wide area over the junction. The interfaces connecting tendons and ligaments to bones are called entheses, while the ones between tendons and muscles are named myotendinous junctions. Several injuries can affect muscles, bones, tendons, or ligaments, and they often occur at the junction sites. For this reason, the main aim of the innovative field of the interfacial tissue engineering is to produce scaffolds with biomaterial gradients and mechanical properties to guide the cell growth and differentiation. Among the several strategies explored to mimic these tissues, the electrospinning technique is one of the most promising, allowing to generate polymeric nanofibers similar to the musculoskeletal extracellular matrix. Thanks to its extreme versatility, electrospinning has allowed the production of sophisticated scaffolds suitable for the regeneration of both the entheses and the myotendinous junctions. The aim of this review is to analyze the most relevant studies that applied electrospinning to produce scaffolds for the regeneration of the enthesis and the myotendinous junction, giving a comprehensive overview on the progress made in the field, in particular focusing on the electrospinning strategies to produce these scaffolds and their mechanical, in vitro, and in vivo outcomes.

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Effects of physical training on sarcomere lengths and muscle-tendon interface of the cervical region in an experimental model of menopause

Cited by 16 publications

References 31 publications

Repercussions on sarcomeres of the myotendinous junction and the myofibrillar type adaptations in response to different trainings on vertical ladder

Repercussions on sarcomeres of the myotendinous junction and the myofibrillar type adaptations in response to different trainings on vertical ladder

Myotendinous junction adaptations to ladder-based resistance training: identification of a new telocyte niche

Tissue Engineering for the Insertions of Tendons and Ligaments: An Overview of Electrospun Biomaterials and Structures

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