Effects of Physical Training on the Myocardium of Oxariectomized LDLr Knockout Mice: MMP 2/9, Collagen I/III, Inflammation and Oxidative Stress

Abstract

Background: The emergence of coronary heart disease is increased with menopause, physical inactivity and with dyslipidemia. Physical training is known to promote the improvement of cardiovascular functions. Objective: To investigate the effects of aerobic physical training on the left ventricle in ovariectomized LDL knockout mice. Methods: Thirty animals were divided into 6 groups (n = 5): Sedentary non-ovariectomized control; Sedentary ovariectomized control; Trained ovariectomized control; Sedentary non-ovariectomized LDL-knockout, sedentary ovariectomized LDL-knockout and trained ovariectomized LDL-knockout. We analyzed the average parameters of apparent density of collagen fibers types I and III, and metalloproteinase type 2 and type 9, were considered significant p < 0.05. Results: The results showed that the proposed exercise protocol altered the volume of type I collagen fibers, altered collagen remodeling parameters (MMP-2), and also reduced the 8-hydroxy-2'-deoxyguanosine (8OHdG) oxidative stress parameter. Conclusion: Moderate intensity aerobic training acts on collagen fiber volume, on collagen remodeling with the reduction of oxidative stress in the left ventricles of ovariectomized LDL-knockout mice.

2-Arachidonoylglycerol Attenuates Myocardial Fibrosis in Diabetic Mice Via the TGF-β1/Smad Pathway

“…Images were taken under a Nikon microscope. The image analysis method is similar to Sirius red staining (the positive area stained in brown) [ 17 ].…”

Aerobic Exercise Modulates Visceral Adipose Tissue of Estrogen Deprived Rats in an Experimental Model of Dyslipidemia

“…Ovariectomy was performed at 9 months as previously reported. 13 Aerobic physical training began 7 days after ovariectomy for 4 weeks on a treadmill with progressive load (1 hour per day, 5Â per week, and 50-70% of maximal running speed) as previous reported. 12,13 At the end of the training protocol, animals were euthanized.…”

“…13 Aerobic physical training began 7 days after ovariectomy for 4 weeks on a treadmill with progressive load (1 hour per day, 5Â per week, and 50-70% of maximal running speed) as previous reported. 12,13 At the end of the training protocol, animals were euthanized. Blood was collected in tubes without anticoagulant and centrifuged at 3000 rpm at room temperature for 10 minutes to obtain the serum of each animal to determine the following biochemical parameters using enzymatic colorimetric assay with spectrophotometric analysis: (A) glucose, (B) triglycerides, and (C) total cholesterol.…”

Treinamento Físico e Função Endotelial em Hipertensos: Efeitos dos Treinamentos Aeróbico e Resistido

“…7 Regarding the mechanisms for exercise-induced endothelial function improvement, the enhancement of nitric oxide bioavailability by reducing its degradation by free radicals is expected. 10 In addition, the balance between damaging and regeneration of endothelial cells has emerged as a promising tool. The reduction of EMP and an increase of EPC (i.e.…”