Effects of physical stimulation with electromagnetic field and insulin growth factor-I treatment on proteoglycan synthesis of bovine articular cartilage

Mattei, Monica De; Pellati, Agnese; Pasello, Michela; Ongaro, Alessia; Setti, Stefania; Massari, Leo; Gemmati, Donato; Caruso, Angelo

doi:10.1016/j.joca.2004.06.012

Cited by 80 publications

(99 citation statements)

References 50 publications

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“…Proteoglycan synthesis was determined by radioactive sulfate incorporation into glycosaminoglycans (GAGs), which are biochemical components of PGs, during 1 or 7 days of culture, as previously reported [De Mattei et al, 2004]. Briefly, 5 mCi/ml of Na 2 -35 SO 4 (2.2 mCi/ml; Amersham Pharmacia Biotech, Buckinghamshire, England) was added to the culture media of both unexposed and exposed explants at time 0 and when the medium was replaced (3rd day).…”

Section: Quantification Of Pg Synthesis and Releasementioning

confidence: 99%

Chondroprotective effects of pulsed electromagnetic fields on human cartilage explants

Ongaro

Pellati

Masieri

et al. 2011

Bioelectromagnetics

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This study investigated the effects of pulsed electromagnetic fields (PEMFs) on proteoglycan (PG) metabolism of human articular cartilage explants from patients with osteoarthritis (OA). Human cartilage explants, recovered from lateral and medial femoral condyles, were classified according to the International Cartilage Repair Society (ICRS) and graded based on Outerbridge scores. Explants cultured in the absence and presence of IL-1β were treated with PEMF (1.5 mT, 75 Hz) or IGF-I alone or in combination for 1 and 7 days. PG synthesis and release were determined. Results showed that explants derived from lateral and medial condyles scored OA grades I and III, respectively. In OA grade I explants, after 7 days exposure, PEMF and IGF-I significantly increased (35) S-sulfate incorporation 49% and 53%, respectively, compared to control, and counteracted the inhibitory effect of IL 1β (0.01 ng/ml). The combined exposure to PEMF and IGF-I was additive in all conditions. Similar results were obtained in OA grade III cartilage explants. In conclusion, PEMF and IGF-I augment cartilage explant anabolic activities, increase PG synthesis, and counteract the catabolic activity of IL-1β in OA grades I and III. We hypothesize that both IGF-I and PEMF have chondroprotective effects on human articular cartilage, particularly in early stages of OA.

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Section: Quantification Of Pg Synthesis and Releasementioning

confidence: 99%

Chondroprotective effects of pulsed electromagnetic fields on human cartilage explants

Ongaro

Pellati

Masieri

et al. 2011

Bioelectromagnetics

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“…Although numerous studies have examined the effects of electromagnetic field on differentiation and gene expression of chondrocytes, few studies have assessed MSCs. 33,34 With the cartilage explants, an electromagnetic field (75 Hz, 1.5 mT) significantly increased not only the 35 S-sulfate incorporation and the cartilage-specific proteins levels but also messenger RNA of aggrecan and type II collagen. Compared to our electromagnetic field system, these studies used much higher frequencies and intensities.…”

Section: Discussionmentioning

confidence: 97%

An Electromagnetic Compressive Force by Cell Exciter Stimulates Chondrogenic Differentiation of Bone Marrow–Derived Mesenchymal Stem Cells

et al. 2006

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In this study, we present a biological micro-electromechanical system and its application to the chondrogenic differentiation of rabbit bone marrow-derived mesenchymal stem cells (MSCs). Actuated by an electromagnetic force, the micro cell exciter was designed to deliver a cyclic compressive load (CCL) with various magnitudes. Two major parts in the system are an actuator and a cartridge-type chamber. The former has a permanent magnet and coil, and the latter is equipped with 7 sample dishes and 7 metal caps. Mixed with a 2.4% alginate solution, the alginate/MSC layers were positioned in the sample dishes; the caps contained chondrogenic defined medium without transforming growth factor-b (TGF-b). Once powered, the actuator coil-derived electromagnetic force pulled the metal caps down, compressing the samples. The cyclic load was given at 1-Hz frequency for 10 min twice a day. Samples in the dishes without a cap served as a control. The samples were analyzed at 3, 5, and 7 days after stimulation for cell viability, biochemical assays, histologic features, immunohistochemistry, and gene expression of the chondrogenic markers. Applied to the alginate/MSC layer, the CCL system enhanced the synthesis of cartilage-specific matrix proteins and the chondrogenic markers, such as aggrecan, type II collagen, and Sox9. We found that the micromechanically exerted CCL by the cell exciter was very effective in enhancing the chondrogenic differentiation of MSCs, even without using exogenous TGF-b.

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“…The modulation of these receptors is possibly one of the mechanisms by which the PEMF counteracts the effect of pro-inflammatory cytokines in explants of cartilage and synovial fibroblasts and prevents the progression to OA [7,11,12]. On the other hand, bovine and human models have shown that PEMFs through a synergy with insulin-like Growth Factor 1 (IGF-1) exerts a pro-anabolic activity enhancing chondrogenic differentiation and synthesis of extra-cellular matrix component [9,10,24].…”

Section: Discussionmentioning

confidence: 99%

“…In vitro and in vivo studies have demonstrated an influence on cartilage metabolism through pro-anabolic and anti-catabolic activity, which has generated interest in the use of PEMFs [7][8][9][10][11][12]. PEMFs were introduced in the clinical setting in the 1970s as a successful method for treatment of non-union and delayed union of fractures; the effects on focal cartilage lesions of the knee remain unknown while studies on OA are equivocal [13][14][15][16][17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Is There any Role for Pulsed Electromagnetic Fields in the Treatment of Early Osteoarthritis of the Knee?

Petrera¹

2013

J Osteopor Phys Act

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Volume 1 • Issue 2 • 1000106institutional review board approval. A written, informed consent was obtained from all the patients. 53 patients with symptomatic cartilage lesions of the knee were treated with PEMFs at our institute. 28 of them (13 male and 15 female) met our study inclusion criteria and were prospectively followed up for a minimum of 2-years post treatment. Inclusion criteria-age between 30 and 60 years; -grade 1-2 cartilage lesion according to the ICRS classification, evaluated by MRI and previous diagnostic arthroscopy ± lavage within 6 months prior to start of treatment ( AbstractObjective: Pulsed Electromagnetic Fields (PEMFs) were introduced in the clinical setting in the 1970s. Proven to be a successful method of treating non-union and delayed union of fractures, its effects on cartilage has remained ambiguous. PEMFs have demonstrated a pro-anabolic and anti-catabolic activity on cartilage metabolism. We hypothesized that the use of PEMFs in patients with symptomatic cartilage lesions of the knee would lead to improved clinical outcomes in an observational study to evaluate the results after 2-years.Methods: 25 patients between the age of 30 and 60 years who underwent treatment with PEMFs for symptomatic cartilage lesions of the knee (grade 1-2 as per ICRS classification) were included in this prospective case series. Patients were evaluated pre-treatment, at 12 months and 24 months using Visual Analogue Scale (VAS) scale for pain, Tegner and KOOS scores.. Results:A significant improvement in all scores was observed at 1-year follow-up (p=0.003). At 2-year follow-up, results deteriorated but were still superior to pre-treatment levels (p=0.04). No adverse reactions were seen. Conclusions:PEMFs in patients with symptomatic isolated cartilage lesions of the knee can cause improvement in symptoms, knee function and activity in the short term. Repetition of treatment annually may improve the long term results.

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Effects of physical stimulation with electromagnetic field and insulin growth factor-I treatment on proteoglycan synthesis of bovine articular cartilage

Cited by 80 publications

References 50 publications

Chondroprotective effects of pulsed electromagnetic fields on human cartilage explants

Chondroprotective effects of pulsed electromagnetic fields on human cartilage explants

An Electromagnetic Compressive Force by Cell Exciter Stimulates Chondrogenic Differentiation of Bone Marrow–Derived Mesenchymal Stem Cells

Is There any Role for Pulsed Electromagnetic Fields in the Treatment of Early Osteoarthritis of the Knee?

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