Effects of physical exercise and social isolation on anxiety-related behaviors in two inbred rat strains

Mazur, Fernando Gabriel; Oliveira, Lígia F. G.; Cunha, Maurício P.; Rodrigues, Ana Lúcia S.; Pértile, Renata Aparecida Nedel; Vendruscolo, Leandro F.; Izídio, Geison S.

doi:10.1016/j.beproc.2017.06.001

Cited by 11 publications

(6 citation statements)

References 74 publications

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“…The increment of locomotor activity in this study may be induced by several factors such as exercise-mediated anxiolytic or a stress response or learn process to a new environment (Lett et al, 2002;Wassum et al, 2011). Mazur et al (2017) had reported that physical exercise on a treadmill could decrease anxiety-like behavior and increase locomotor activity in rats. Furthermore, the exercise-induced increase in cardiovascular fitness or muscular endurance may facilitate better locomotor capability.…”

Section: Discussionmentioning

confidence: 71%

Aerobic Exercise Altered Locomotor Activity and Induced D1 Dopamine Receptor and Brain Derived Neurotropic Factor (BDNF) Expressions

Lesmana¹

2019

PVJ

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Exercise contributes in improving mental health and alleviates depression, anxiety, and cognitive impairment by facilitating neuronal remodeling (Park H, Poo MM. 2013). The involvement of brain derived neurotropic factor (BDNF) and D1 dopamine receptor (D1DR) has been reported. On the other hand, a limited number of information is available on the effect exercise with altered intensity on locomotor activity. This study explored the effect of altered training intensity on adult male rat locomotor activity, which may be partly regulated by neuronal remodeling through dopamine and BDNF signalling. Male adult Wistar rats were divided into 4 groups: stationary control, aerobic (sub LT), lactate threshold (LT), and anaerobic training groups (Supra LT). They were trained for 14 days followed by measuring the locomotor activity in their home cage from day 14 to 16. Rats were then sacrificed under anesthesia, and the striatum was separated for RT-PCR and Western blot analysis. We found that two-weeks training induced hyperactivity during night regardless of the training intensity. Only aerobic training group showed increased D1DR mRNA and protein levels; BDNF mRNA levels were also increased. The phosphorylation of Tropomyosin receptor kinase (Trk) A and TrkB was also altered significantly (TrkA, all training groups; TrkB, only aerobic group). In summary, two weeks training with various intensities induces hyperactivity in rats during night. Among various training protocols, only aerobic training activated D1DR and BDNF signaling pathways. These results partly revealed the mechanism how exercise with different intensity can alter behavior in rats. In addition, our study may have clinical relevance to clarify the role of exercise, particularly aerobic training, to improve mental health. To Cite ThisArticle: Lesmana R, Pratiwi YS, Goenawan H, Hadi EJ, Tarawan VM, Sylvana N, Setiawan I, Purba A, Supratman U and Koibuchi N, 2019. Aerobic exercise altered locomotor activity and induced D1 dopamine receptor and Brain Derived Neurotropic Factor (BDNF) expressions. Pak Vet J, 39(3): 341-346. http://dx.

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Section: Discussionmentioning

confidence: 71%

Aerobic Exercise Altered Locomotor Activity and Induced D1 Dopamine Receptor and Brain Derived Neurotropic Factor (BDNF) Expressions

Lesmana¹

2019

PVJ

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“…Then, 2 hr after IA test the rats were exposed to a 5‐min session in a novel square arena with the floor divided into 16 quadrants, with four of these in a central position. A decrease in the number of crossings of the central quadrants is used as an index of anxiety‐like behavior (Hazim et al., 2014; Mazur et al., 2017). The use of this alternative paradigm to re‐evaluate the anxiety‐like behavior was because the EPM could be ineffective in a second session (File et al., 1990).…”

Section: Resultsmentioning

confidence: 99%

Behavioral tagging as a mechanism for aversive‐memory formation under acute stress

Cunha

Tintorelli

Correa

et al. 2021

Eur J of Neuroscience

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Stress is used to describe physiological and behavioral changes elicited by events that are interpreted as threatening or challenging to an individual (McEwen, 2000;Ness & Calabrese, 2016). Several substances are released in response to stressful events, as glucocorticoids and noradrenaline, which act over numerous neural networks enabling organisms to successfully cope with the stressor (Joëls & Baram, 2009;McIntyre et al., 2012). Diverse brain areas as

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“…Because this occurred in both water-drinking and EtOH-drinking rats and it has now occurred across strains, we hypothesize it is due to the stress of single-housing. Indeed, prolonged social isolation during adulthood increases pain and negative affect in both humans and animals(Baker et al, 2022; Bannon et al, 2021; Blass et al, 1987; Mazur et al, 2017; Santini et al, 2020; Taheri Zadeh et al, 2021; Tuboly et al, 2009; Yamada et al, 2021). The effect of EtOH drinking on pain sensitivity and affective behavior was examined next.…”

Section: Discussionmentioning

confidence: 99%

Chronic Alcohol Drinking Drives Sex-Specific Differences in Affective Behavior and Medial Prefrontal Cortex Activity in CRF1:Cre:Tdtomato Transgenic Rats

Quadir

Arleth

Cone

et al. 2022

Preprint

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Alcohol use disorders are complex conditions characterized in part by excessive ethanol (EtOH) drinking and withdrawal-induced malaise. The corticotropin releasing factor-1 receptor (CRF1) has been implicated in EtOH drinking, affective states, and pain sensitivity; often in a sex-dependent manner. The studies presented here investigate the associations between baseline behavior, chronic intermittent EtOH intake, and withdrawal-induced affective behavior in male and female CRF1:cre:tdTomato rats. There were no sex differences in basal affective state or thermal sensitivity, as measured by the splash test, novelty suppressed feeding test, and Hargreaves, respectively. However, female rats displayed increased mechanical sensitivity, measured by the Von Frey test. Following baseline testing, rats underwent voluntary EtOH or water drinking under intermittent access conditions. Female rats consumed significantly more EtOH, but only during the first week. There were no group effects of EtOH on behavioral tests, but all of the rats demonstrated increased malaise upon repeated testing. There were significant individual differences where affective behavior positively correlated with negative affect in both sexes. There were also significant correlations between EtOH intake and CRF1-cFos co-expression in the infralimbic cortex and basolateral amygdala. EtOH decreased CRF1+ expression in the lateral amygdala, but there were no significant group effects of EtOH on cFos or CRF1-cFos co-expression in the medial prefrontal cortex or amygdala. Together, these results illustrate the complex interplay between affect, pain sensitivity, EtOH drinking, and the role of CRF1 containing neurons in mediating these behaviors.

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Effects of physical exercise and social isolation on anxiety-related behaviors in two inbred rat strains

Cited by 11 publications

References 74 publications

Aerobic Exercise Altered Locomotor Activity and Induced D1 Dopamine Receptor and Brain Derived Neurotropic Factor (BDNF) Expressions

Aerobic Exercise Altered Locomotor Activity and Induced D1 Dopamine Receptor and Brain Derived Neurotropic Factor (BDNF) Expressions

Behavioral tagging as a mechanism for aversive‐memory formation under acute stress

Chronic Alcohol Drinking Drives Sex-Specific Differences in Affective Behavior and Medial Prefrontal Cortex Activity in CRF1:Cre:Tdtomato Transgenic Rats

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