Effects of photobiomodulation therapy in dentoalveolar-derived mesenchymal stem cells: a review of literature

Abstract

This review aims to present and discuss the published data on the application of photobiomodulation therapy (PBMT) on cultured cells, including dentoalveolar-derived mesenchymal stem cells (ddMSCs). The knowledge of the effects of PBMT on ddMSCs is of importance for future application in tissue engineering approaches aiming to regenerate missing dental tissues, among others. The tissue engineering, which is based on the triad stem cells, growth factors, and scaffolds, presents shortcomings that could be circumvented by the effects of the PBMT. Thus, our research group has proposed the PBMT as a fourth element of tissue engineering. This proposition is based on the published data on the effects of PBMT on different cell types, including ddMSCs. Future perspectives on PBMT and ddMSCs will be presented showing translational studies of tissue regeneration induced by PBMT. The experience of our research group supported by other studies of the literature shows the beneficial effects of PBMT. Moreover, this review will show that PBMT on ddMSCs and tissue regeneration is still a huge field for future investigations not only in vitro but also in vivo.

Effects of different culture media, cell densities and adhesion periods on stem cells from human exfoliated deciduous teeth after photobiomodulation

“…However, with the adhesion period of 48 h, this SHED density showed a decreasing in viability, 6 and 24 h after PBM. The rationale behind this fact is that although an inadequate cell density are not able to express satisfactory rates of viability, the greater cell density for a longer period resulted in greater cell confluency in the well, which jeopardize the microenviron ment due to contact inhibition of the cell monolayer, leading to stress and cell death [4,19]. Based on this assumption, higher cell density and shorter adhesion period led to greater cell viability.…”

“…The stem cells from human exfoliated deciduous teeth (SHED) are phenotypically different from other stem cells of body tissues [1,2]. Currently studies on SHED explores the clonogenic and differentiation potential into many cell types, as: neural cells, adipocytes, and odontoblasts [3][4][5].…”

“…The benefits of photobiomodulation (PBM) are based on anti-inflammatory effects, stimulus of the cell proliferation, collagen synthesis, and improvement of vascularization [4,6,7]. In vitro studies on SHED and Laser reveal that, after PBM, SHED show increased viability and proliferation [3,[7][8][9].…”

Photobiomodulation therapy and vitamin C on longevity of cell sheets of human dental pulp stem cells

“…Thus, at the time of CS transplantation into a patient, PBMT could be applied without harming the integrity of the CSs. Thus, all of the biological advantages of PBMT could be taken, such as prevention of cell death (Moreira et al, 2017), cell growth improvement, (Marques et al, 2016(Marques et al, , 2017 and VEGF synthesis (Park et al, 2015), which, in turn, would promote nutritious conditions for these cells. Additionally, PBMT can activate latent growth factors, such as TGF-ß1 (Arany et al, 2014).…”

“…During the last three decades, tissue engineering based on triad stem cells (SCs), growth factors, and scaffolds has sought new regenerative solutions that are capable of restoring, replacing, maintaining, or enhancing biological tissue functions (Skalak & Fox, 1988, Langer & Vacanti, 1993. Photobiomodulation therapy (PBMT) may improve cell processes that are relevant to tissue regeneration, such as survival, proliferation, migration, and differentiation (Arany et al, 2014;Marques et al, 2016Marques et al, , 2017) by acting on cell metabolism, especially on cells under oxidative stress (Hamblin & Demidova, 2006). Moreover, PBMT can also prevent cell death (Moreira et al, 2011(Moreira et al, , 2017 and prolong cell longevity both in vitro and in vivo (Park, Mondal, Chung, & Ahn, 2015).…”