Effects of Photobiomodulation on Stem Cells Important for Regenerative Medicine

Chang, Seung Jun; Carpena, Nathaniel T.; Kang, Bong Jin; Lee, Min Young

doi:10.25289/ml.2020.9.2.134

Cited by 7 publications

(6 citation statements)

References 55 publications

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“…47 While studies used red and near-infrared wavelengths for the combination of UCMSC and PBM, red wavelengths (620, 625, 633, 635 nm) proved effective for inducing blood vessels and bone formations. 14,[48][49][50] Table 4 summarizes the parameters and results of studies on the effect of LLLT on UCMSCs.…”

Section: Umbilical Cord Blood-derived Mesenchymal Stem Cells (Ucmscs)mentioning

confidence: 99%

“…Observations in vitro and in vivo of PBM and MSCs combination showed anti-inflammatory effects, stimulating lesion repair, and other effects, such as reduction of hypoxic damage. 14,15 However, despite the effectiveness of low-power laser irradiation on cellular therapy, the clinical use of PBM has not been widely accepted yet, as laser on MSCs regarding its molecular, cellular, and tissue mechanisms of action remain poorly understood. 16,17 This paper aims to review the effects of PBM on the proliferation, differentiation, and migration of several types of MSCs, identify trends in how these cells respond, and the effects on its therapeutic efficacy, as explored in previous studies reported in the literature.…”

Section: Introductionmentioning

confidence: 99%

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Photobiomodulation improves the therapeutic efficacy of mesenchymal stem cells in regenerative medicine

Woo¹,

Abueva²

2022

Medical Lasers

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Mesenchymal stem cells (MSCs) are multipotent stromal cells that can differentiate into many other cell types typically isolated from adult tissue sources. The therapeutic potential of MSCs is considerable in regenerative medicine and has advanced over the years. Consequently, methods and tools that improve the therapeutic efficacy of MSCs have also been devised and utilized. Recent strategies used to enhance the therapeutic efficacy of MSC include photobiomodulation (PBM), which can increase proliferation and enhance differentiation into target cell types. PBM is a non-thermal, non-invasive method that utilizes light from coherent or non-coherent sources to influence metabolic activity at the cellular level and has been found to increase cell proliferation and migration in vitro, in vivo, and in clinical studies. However, little is known of the mechanism responsible for the effects of PBM on MSCs or its potential as an adjunctive treatment, which obscures appreciation of its regenerative applications. This review summarizes reports on the effects of PBM on different MSCs and provides perspectives on the ability of PBM to improve the therapeutic efficacy of MSCs in regenerative medicine.

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Section: Umbilical Cord Blood-derived Mesenchymal Stem Cells (Ucmscs)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Photobiomodulation improves the therapeutic efficacy of mesenchymal stem cells in regenerative medicine

Woo¹,

Abueva²

2022

Medical Lasers

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“…As has been introduced earlier by our group, PBM which is non-invasive stimulation with varying wavelength has many beneficial effects such as differentiation, proliferation and migration. 10 Recently, PBM is thought to have clinical potential for regenerative medicine. PBM modulation on stem cells were published for animal derived mesenchymal stem cell (MSC)s, umbilical cord bloodderived MSCs, human bone marrow stem cells, human dental pulp stem cells and human adipose derived stem cells.…”

Section: Photobiomodulation (Pbm) Effect On Stem Cellsmentioning

confidence: 99%

“…PBM modulation on stem cells were published for animal derived mesenchymal stem cell (MSC)s, umbilical cord bloodderived MSCs, human bone marrow stem cells, human dental pulp stem cells and human adipose derived stem cells. Please refer to the paper published 2020 by Chang et al 10 for detailed tables for each studies. Considering these publications, it is feasible to believe that PBM could strengthen the stem cell and might overcome the obstacles which were raised previously (small survival and differentiation possibility).…”

Section: Photobiomodulation (Pbm) Effect On Stem Cellsmentioning

confidence: 99%

Hearing loss, stem cells, and photobiomodulation

Lee

2022

Medical Lasers

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Sensorineural hearing loss, that is, hearing loss due to loss of sensory cells and related neural structures, greatly impacts the current socioeconomic environment. Trials to regenerate these structures are ongoing, but evidence regarding therapeutic options is still lacking. Stem cell therapy is a promising therapeutic option for restoring hearing in patients with sensorineural hearing loss. Potential stem cell-based regenerative therapies target either cochlear hair cells, which perform a primary role during sound transduction to brain, or neural structures connecting to the organ of Corti (especially inner hair cells). However, several obstacles remain to be overcome, such as a low survival rate and a complicated differentiation process. Photobiomodulation (PBM) is a non-invasive technique that involves stimulation with different wavelengths and has many beneficial effects on stem cell differentiation, proliferation, and migration. Several studies have demonstrated that PBM offers a potential means of overcoming these obstacles. However, more detailed, well-constructed experiments are required to expand the possibilities offered by PBM.

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“…We have reported that LED irradiation can result in various biological effects, such as mouse hepatocyte proliferation and colon cancer cell autophagy [3,4]. The effect of LED irradiation at different wavelengths and energy densities also improves the regenerative capacity of various types of MSC [5,6]. Green LED (GLED) irradiation promotes the differentiation of MSCs into neurons and osteoblasts [2,7].…”

Section: Introductionmentioning

confidence: 99%

Effects of green light-emitting diode irradiation on hepatic differentiation of hepatocyte-like cells generated from human adipose-derived mesenchymal cells

Waki

Saito

Chen

et al. 2023

Preprint

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Background Light-emitting diode (LED) irradiation has been used in the differentiation of mesenchymal stem cells into a variety of cell types. This study investigated the effect of green LED (GLED) irradiation on the differentiation of adipocyte-derived mesenchymal cells into hepatocyte-like cells (HLCs) and the mechanism of its action. Methods HLCs in the hepatocyte maturation phase were irradiated with GLED (520 nm, 21 W/m2, 5 min/day for 10 days). The cells were then assessed for expression of hepatocyte maturity genes and opsin 3 (OPN3), hepatocyte function, viability, apoptosis, and levels of reactive oxygen species (ROS), intracellular adenosine triphosphate (ATP) and calcium ions (Ca2+). Results GLED irradiation increased Alpha-1 antitrypsin and Ornithine transcarbamylase gene expression, promoted Cytochrome P450 3A4 activity and urea synthesis, and elevated intracellular ROS, ATP and Ca2+ levels. OPN3 expression was significantly more upregulated in GLED-irradiated HLCs than in the non-irradiated HLCs. No significant difference in cell viability or apoptosis was observed between GLED-irradiated and non-irradiated HLCs. Conclusions GLED irradiation can promote hepatocyte maturation and functions through OPN3. GLED irradiation also stimulated mitochondrial function via Ca2+/ATP/ROS activation. GLED irradiation has potential to support cell-based transplantation in patients.

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Effects of Photobiomodulation on Stem Cells Important for Regenerative Medicine

Cited by 7 publications

References 55 publications

Photobiomodulation improves the therapeutic efficacy of mesenchymal stem cells in regenerative medicine

Photobiomodulation improves the therapeutic efficacy of mesenchymal stem cells in regenerative medicine

Hearing loss, stem cells, and photobiomodulation

Effects of green light-emitting diode irradiation on hepatic differentiation of hepatocyte-like cells generated from human adipose-derived mesenchymal cells

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