Effects of photo-stimulation with laser or LED on the composition of Xanthan gum produced in media containing distilled water or dialyzed or not produced water by means of Raman spectroscopy

Production and viscosity of Xanthan Gum are increased by LED irradiation of X. campestris cultivated in medium containing produced water of the oil industry

“…A previous report using Raman spectroscopy from our group has shown positive photo-induced effects on X. campestris [19]. The study of Xanthan gum productions either using distilled water or DPW and irradiated or not by either Laser (λ = 660nm) or LED (λ = 630nm ± 2nm) were indicative of increased amounts of pyruvyl-mannoses in Xanthan gum produced with DPW [18,19].…”

“…A previous report using Raman spectroscopy from our group has shown positive photo-induced effects on X. campestris [19]. The study of Xanthan gum productions either using distilled water or DPW and irradiated or not by either Laser (λ = 660nm) or LED (λ = 630nm ± 2nm) were indicative of increased amounts of pyruvyl-mannoses in Xanthan gum produced with DPW [18,19]. It was also found that photo-stimulation caused an additional increase in the pyruvylation of mannose residues, thus, the increase in viscosity obtained in this study in the groups irradiated by LED may be a result of such modifications (Fig.…”

“…Enhancement of XG production was observed when using distilled water in the culture medium. In contrast, both pyruvate acetyl mannose content increased in the dialyzed-produced water culture [19].…”

“…The Xanthomonas campestris IBSBF 2103 strain (Culture Collection of the Biological Institute -Campinas, SP, Brazil) was used. To ensure genomic integrity and reduce the risk of fluctuations due to genetic alterations of the strain [20], a stock conservation was made in cryogenic tubes of 2 mL as previously described by our team [19].…”

“…Two hundred and twenty-five microliters of homogenized X. campestris culture were inoculated in 22.5 mL of YM medium and incubated in a stirring table at 28°C at 150 rpm [18,19]. After 24-h of growth, 10 mL of the culture were inoculated in 90 mL of modified MSM medium as previously described [18,21].…”