Effects of Phosphoinositide Cycle Modifiers on Apoptosis of Peritoneal Macrophages

Aksenova, O N; Trofimov, V. A.; Lychkina, T N

doi:10.1023/b:bebm.0000042716.14439.5a

Cited by 1 publication

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“…001% isopropanol (IPA)), w45 min before induction of apoptosis with H 2 O 2 during different periods of times. H 2 O 2 was diluted in culture medium without serum at a final concentration of 1 mM in each assay (Aksenova et al 2004, Pronsato et al 2010. After treatments, cells were lysed using a buffer composed of 50 mM Tris-HCl pH 7 .…”

Section: Methodsmentioning

confidence: 99%

Testosterone exerts antiapoptotic effects against H2O2 in C2C12 skeletal muscle cells through the apoptotic intrinsic pathway

Pronsato¹,

Boland²,

Milanesi³

2012

Journal of Endocrinology

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Experimental data indicate that apoptosis is activated in the aged skeletal muscle, contributing to sarcopenia. We have previously demonstrated that testosterone protects against hydrogen peroxide (H 2 O 2 )-induced apoptosis in C2C12 muscle cells. Here we identified molecular events involved in the antiapoptotic effect of testosterone. At short times of exposure to H 2 O 2 cells exhibit a defense response but at longer treatment times cells undergo apoptosis. Incubation with testosterone prior to H 2 O 2 induces BAD inactivation, inhibition of poly(ADP-ribose) polymerase cleavage, and a decrease in BAX levels, and impedes the loss of mitochondrial membrane potential, suggesting that the hormone participates in the regulation of the apoptotic intrinsic pathway. Simultaneous treatment with testosterone, H 2 O 2 , and the androgen receptor (AR) antagonist, flutamide, reduces the effects of the hormone, pointing to a possible participation of the AR in the antiapoptotic effect. The data presented allow us to begin to elucidate the mechanism by which the hormone prevents apoptosis in skeletal muscle.

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Section: Methodsmentioning

confidence: 99%