Effects of Phosphoethanolamine Supplementation on Mitochondrial Activity and Lipogenesis in a Caffeine Ingestion Caenorhabditis elegans Model

Min, Hyemin; Youn, Esther; Kim, Jae-Hoon; Son, Su Young; Lee, Choong Hwan; Shim, Yhong–Hee

doi:10.3390/nu12113348

Cited by 16 publications

(15 citation statements)

References 77 publications

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“…Western blot analysis was performed as described previously [ 28 ]. Whole animal protein extracts were obtained from 300 gravid adult hermaphrodites of each condition per gel well.…”

Section: Methodsmentioning

confidence: 99%

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Nicotinamide Supplementation Improves Oocyte Quality and Offspring Development by Modulating Mitochondrial Function in an Aged Caenorhabditis elegans Model

et al. 2021

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Aging is associated with a decline in the quality of biological functions. Among the aging processes, reproductive aging is a critical process because of its intergenerational effects. However, the mechanisms underlying reproductive aging remain largely unknown. Female reproductive aging is the primary reason for limited fertility in mammals. Therefore, we attempted to investigate a modulator that can control female reproductive aging using a Caenorhabditis elegans model. In the present study, we examined the role of nicotinamide (NAM) in oocyte quality and offspring development. The levels of reactive oxygen species (ROS) and oxidative stress responses in aged oocytes, embryonic lethality, and developmental growth of the offspring were examined with maternal NAM supplementation. Supplementation with NAM improved oocyte quality, decreased embryonic lethality, and promoted germ cell apoptosis. Furthermore, NAM supplementation in aged mothers reduced ROS accumulation and improved mitochondrial function in oocytes. Consequently, the developmental growth and motility of offspring were improved. These findings suggest that NAM supplementation improves the health of the offspring produced by aged mothers through improved mitochondrial function. Taken together, our results imply that NAM supplementation in the aged mother improves oocyte quality and protects offspring by modulating mitochondrial function.

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“…Western blot analysis was performed as described previously [ 28 ]. Whole animal protein extracts were obtained from 300 gravid adult hermaphrodites of each condition per gel well.…”

Section: Methodsmentioning

confidence: 99%

“…To examine the effect of maternal NAM supplementation on mitochondrial ROS, CellROX ® Green (Invitrogen, Carlsbad, CA, USA) staining was performed as previously described [ 28 ]. CellROX ® Green is a fluorogenic probe for measuring oxidative stress in live cells.…”

Section: Methodsmentioning

confidence: 99%

Nicotinamide Supplementation Improves Oocyte Quality and Offspring Development by Modulating Mitochondrial Function in an Aged Caenorhabditis elegans Model

et al. 2021

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“…Mitotracker assay is an advantageous method to access mitophagy as well as mitochondrial content [32]. We employed mitotracker based mitochondrial staining to check the mitochondrial content in knock down condition of nhx-2.…”

Section: Measurement Of Mitochondrial Content With the Help Of Mitotrackermentioning

confidence: 99%

“…Standard protocol with modi cations, have been utilized for ATP detection in C. elegans. Brie y, around 300 worms were collected from each group and proceeded for protein isolation as described above [32]. The Bradford reagent is utilized for the measurement of the concentration of protein.…”

Section: Atp Measurementmentioning

confidence: 99%

Targeted Downregulation of Estradiol Binding Na+/H+ Exchanger Nhx-2, Mimics Calorie Restriction, Extends Reproductive Longevity and Ameliorates Effects Associated With Alpha Synuclein Aggregation In C. Elegans

Shukla

Kumar²,

Sarkar³

et al. 2021

Preprint

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Setting in of reproductive senescence (RS) gives rise to several changes, making aged individuals susceptible to multiple disorders including neurodegenerative diseases, cardiovascular ailments and bone disorders amongst others. The present study, employing transgenic C. elegans that expresses ‘human’ alpha synuclein, endeavors to decipher the association of reproductive senescence with age-associated neurodegenerative diseases and behavioral ageing, under normal conditions and after being probed with estradiol. We carried out RNAi induced silencing of a subset of 22 genes that are known to delay RS, followed by studies on alpha-Synuclein aggregation and associated effects. These studies led us to functional characterization of the Na+/H+ exchanger; nhx-2, expressed exclusively in gut. We found that RNAi of nhx-2 not only ameliorates the effects associated with alpha-Synuclein aggregation, but it also attunes effects related to behavioral aging including that of reproductive health-span and neuroprotection via mimicking dietary restriction, as it alters food absorption from the gut. We further elucidated that these effects are Sir-2.1 driven as nhx-2 knock out did not delay reproductive senescence in knock down condition of sir-2.1. To substantiate our findings, we performed whole transcriptome analysis in nhx-2 mutant strain. Our data revealed differential expression of 61 out of 62 hallmark genes of CR described by GenDR, in knock out condition of nhx-2. As estradiol plays a central role in both reproductive health as well as neuronal health, we subjected worms to exogenous estradiol treatment and observed that it led to elevated levels of nhx-2. Studies on structural binding analysis demonstrated significant binding potential of estradiol receptor NHR-14 with nhx-2 gene and ChIP analysis revealed that estradiol treatment gives rise to enhanced NHX-2 levels through inducing the promoter specific histone H3 acetylation (H3K9) and lysine methylation (H3K4me3). These studies identify nhx-2 as an important modulator that extends reproductive longevity and ameliorates effects associated with alpha synuclein aggregation in C elegans.

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“…elegans. Briefly, around 300 worms were collected from each group and proceeded for protein isolation as described above [32]. The Bradford reagent is utilized for the measurement of the concentration of protein.…”

Section: Atp Measurementmentioning

confidence: 99%

Targeted downregulation of estradiol binding Na+/H+ exchanger nhx-2, mimics calorie restriction, extends reproductive longevity and ameliorates effects associated with alpha synuclein aggregation in C. elegans

Shukla

Kumar

Sarkar

et al. 2020

Preprint

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Setting in of reproductive senescence (RS) gives rise to several changes, making aged individuals susceptible to neurodegenerative diseases, cardiovascular and bone disorders amongst others. The present study deciphers the association of reproductive senescence, presence/absence of the sex hormone estradiol with age-associated neurodegenerative diseases. We employed RNAi induced silencing of a subset of 22 genes that are known to delay RS, followed by studies on alpha-Synuclein aggregation and associated effects in the transgenic C. elegans. These studies led us to fuctional characterisation of the Na+/H+ exchanger, expressed exclusively in gut. We found that RNAi of nhx-2 ameliorates the affects associated with alpha-Synuclein aggregation via mimicking dietary restriction as it alters food absorption from the gut. Our studies further elucidated that such effects are Sir-2.1 driven as nhx-2 RNAi did not delay reproductive senescence when sir-2.1 was silenced concurrently. As estradiol plays a central role in both reproductive health as well as neuronal health, we performed structural binding analysis that demonstrated the binding potential of the estradiol receptor NHR-14 with nhx-2 gene. Hence, we treated the worms with estradiol and observed that the transcription levels of nhx-2 were elevated above the endogenous level. To unravel the underlying molecular mechanism of induction we performed ChIP analysis and it revealed that estradiol treatment gives rise to enhanced NHX-2 levels through inducing the promoter specific histone H3 acetylation (H3K9) and lysine methylation (H3K4me3).Graphical abstractHIGHLIGHTSSilencing of a sodium proton antiporter nhx-2 of gut ameliorates effects associated with alpha-Synuclein aggregation via mimicking dietary restriction in C. elegans.We have established a genetic cross to construct a strain that expresses mCherry SIR-2.1 ubiquitously and α-Synuclein in muscles, that assists in corroborating the link between NHX-2 and SIR-2.1.Human 17-β-Estradiol treatment induces the expression of nhx-2, through inducing the promoter specific histone H3 acetylation (H3K9) and lysine methylation (H3K4me3).Effects associated with nhx-2, including prolonged reproductive span and neuroprotective effects, are SIR-2.1 driven.nhx-2 silencing decreases alpha-Synuclein aggregation however estradiol mediated overexpression above the endogenus level, does not amend the aggregation any further.

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Effects of Phosphoethanolamine Supplementation on Mitochondrial Activity and Lipogenesis in a Caffeine Ingestion Caenorhabditis elegans Model

Cited by 16 publications

References 77 publications

Nicotinamide Supplementation Improves Oocyte Quality and Offspring Development by Modulating Mitochondrial Function in an Aged Caenorhabditis elegans Model

Nicotinamide Supplementation Improves Oocyte Quality and Offspring Development by Modulating Mitochondrial Function in an Aged Caenorhabditis elegans Model

Targeted Downregulation of Estradiol Binding Na+/H+ Exchanger Nhx-2, Mimics Calorie Restriction, Extends Reproductive Longevity and Ameliorates Effects Associated With Alpha Synuclein Aggregation In C. Elegans

Targeted downregulation of estradiol binding Na+/H+ exchanger nhx-2, mimics calorie restriction, extends reproductive longevity and ameliorates effects associated with alpha synuclein aggregation in C. elegans

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