Effects of phosphodiesterase inhibitors and cyclic nucleotides on sperm respiration and motility

Lardy, Henry A.; Garbers, David L.; Lust, W. David; First, N. L.

doi:10.1021/bi00786a015

Cited by 232 publications

(7 citation statements)

References 29 publications

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“…Caffeine (a component of BO sperm wash), lignocaine and procaine (both local anaesthetic agents) have been used to induce hyperactivation in numerous mammalian species by employing various mechanisms of action. Caffeine enhances sperm motility by increasing intracellular calcium by translocation from internal calcium stores (Hong et al, 1985;Ho & Suarez, 2001) or by inhibiting phosphodiesterase, which results in increased cyclic adenosine monophosphate and protein kinase A levels (Garbers et al, 1971;Bender & Beavo, 2006). Lignocaine has been shown to cause an increase in the concentration of intracellular Ca 2+ in sensory neurons from influx through the plasma membrane and release from Ca 2+ internal stores (Gould et al, 1998) and to cause sperm hyperactivation in human sperm (Bennett et al, 1992).…”

Section: Figurementioning

confidence: 99%

Quantitative sperm characteristics of Tankwa goats with special reference to hyperactivated motility

Ngcauzele¹,

Horst²,

Kotzé³

et al. 2021

SA J. An. Sci.

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Computer-assisted semen analysis (CASA) is an automated and objective method of evaluating structural (e.g. morphology) and functional sperm parameters (e.g. motility and hyperactivation). Sperm hyperactivation is essential for successful fertilization and is thus an important aspect in determining the fertility potential of a male. In the current study, CASA was used for standard semen analysis and for comparison of the ability of phosphate buffered saline (PBS), BO sperm wash (10 mM caffeine), 4% lignocaine, and 5 mM procaine hydrochloride to induce hyperactivation in Tankwa goat spermatozoa. Twenty-nine ejaculates were collected from randomly selected male goats by electroejaculation. Although none of the four media affected percentage total sperm motility, lignocaine caused a significant decrease (P >0.05) in percentage progressive motility. Exposure to procaine resulted in an increase in swimming speed (P ≤0.05) and star-spin motility tracks, which are typical of sperm hyperactivation. Using PBS and procaine motility data from individually selected spermatozoa, receiver operator characteristic curves were constructed to distinguish the kinematic parameters employed as cut-off values for sperm hyperactivation. PBS and BO sperm wash did not induce hyperactivation (0.1 + 0.2% and 0.04  0.2% respectively), while lignocaine induced little hyperactivation (3.4 + 3.0%) and procaine hydrochloride had the highest percentage hyperactivation (25.3 + 13.6%). The large variation in hyperactivation (0–54.5%) may reflect inter-individual differences in sperm quality among these males. This study indicated procaine hydrochloride was the most promising hyperactivation-inducing medium for Tankwa goat spermatozoa and should be considered for similar assessments in other animal species Keywords: computer-aided sperm analysis, procaine hydrochloride, sperm kinematics, sperm morphometry, sperm motility

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Section: Figurementioning

confidence: 99%

Quantitative sperm characteristics of Tankwa goats with special reference to hyperactivated motility

Ngcauzele¹,

Horst²,

Kotzé³

et al. 2021

SA J. An. Sci.

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“…In frozen-thawed boar semen, the use of caffeine in the thawing solution improved the progressive motility, straightness, and linearity of sperm movement (Yamaguchi e Funahashi, 2012;Yamaguchi et al, 2013). It is known that caffeine positively affects sperm function by upregulating glycolysis via a buildup of cyclic adenosine monophosphate (cAMP), which generates adenosine triphosphate (ATP), used to power sperm movement (Lardy et al, 1971). Rhemrev et al (2001) suggested that the motility of the spermatozoa was related to their fertilizing capacity and the sperm motility was dependent on mitochondrial function.…”

Section: Introductionmentioning

confidence: 99%

Addition of chlorogenic acid and caffeine during the processing of cooled boar semen

Pereira

Rocha

Teles

et al. 2019

Arq. Bras. Med. Vet. Zootec.

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A study was conducted to evaluate the effect of chlorogenic acid (ChA) added pre-cooling and its combination with caffeine added during warming on cooled-stored boar semen parameters. Ten ejaculates were diluted in commercial extender with or without 4.5mg/ml ChA and stored at 15°C. After 0, 24 and 72 hours of storage, aliquots of these doses were taken and incubated at 37°C in the presence or absence of 8.0mM caffeine. Semen quality was evaluated after 10 and 120 minutes of incubation. The ChA increased (P <0.01) the sperm motility, viability, acrosomal integrity and the percentage of spermatozoa with high mitochondrial activity (PMHA), however, decreased (P <0.01) the malondialdehyde (MDA) concentration. Caffeine increased (P<0.05) the sperm motility, viability, PMHA and the MDA concentration and reduced (P <0.05) the acrosome integrity. When associated (ChA+caffeine), there was an increase (P <0.05) in sperm motility and viability, PMHA and acrosome integrity. The addition of ChA to the dilution medium improves the quality of the swine inseminating doses. The addition of caffeine during re-warming is only recommended when the semen is stored for prolonged periods (72h), and the inseminating dose should be used immediately after its addition.

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“…cAMP plays a role in the control of motile spermatozoa. Nevertheless, its numerous protective mechanisms are less clear (20,21). …”

Section: Introductionmentioning

confidence: 99%

Evaluation of the Effect of Pentoxifylline on Cisplatin-Induced Testicular Toxicity in Rats

et al. 2017

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Chemotherapy is associated with male infertility. Cisplatin (cis-diamminedichloro-platinum (II) (CDDP) as a chemotherapy medication used to treat a number of cancers has been reported to most likely induce testicular toxicity. Administration of antioxidants, such as pentoxifylline (PTX) may reduce some Adverse Drug Reactions (ADRs) of CDDP. Therefore, this study investigated the potentially protective effects of PTX on CDDP-induced testicular toxicity in adult male rats. For this purpose, 42 male rats were randomly divided into 7 groups. The rats were orally pretreated with PTX at the 3 doses of 75, 150, and 300 mg/kg once a day for 14 successive days. On the 14th day of the study, they were intraperitoneally (IP) administered with a single dose of CDDP (7 mg/kg). Finally, the sperm/testis parameters, serum levels of reproductive hormones, including testosterone, Luteinizing Hormone (LH), and Follicle Stimulating Hormone (FSH) as the pivotal endocrine factors controlling testicular functions, and histopathological changes of testis tissue were examined. Pretreatment with the two doses of 75 and 150 mg/kg PTX indicated significant increases in the sperm count and motility induced by CDDP administration. The right and significantly left testis weights were decreased following the treatment with 300 mg/kg of PTX plus CDDP. However, 75 mg/kg of PTX plus CDDP showed the best near-to-normal histopathological features. The results demonstrated that PTX alone enhanced some parameters, such as the sperm count, while reducing other parameters, including sperm fast motility and germ layer thickness. Furthermore, despite testosterone or LH levels, the mean serum FSH level was significantly augmented by the doses of 75 and 150 mg/kg. It was concluded that PTX administration cannot reduce CDDP-induced testicular toxicity even at high doses (e.g., 300 mg/kg), while it seemed to partially intensify CDDP toxicity effects at a dose of 75 mg/kg. Thus, further research is required in this regard.

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Effects of phosphodiesterase inhibitors and cyclic nucleotides on sperm respiration and motility

Cited by 232 publications

References 29 publications

Quantitative sperm characteristics of Tankwa goats with special reference to hyperactivated motility

Quantitative sperm characteristics of Tankwa goats with special reference to hyperactivated motility

Addition of chlorogenic acid and caffeine during the processing of cooled boar semen

Evaluation of the Effect of Pentoxifylline on Cisplatin-Induced Testicular Toxicity in Rats

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