Effects of phosphatidylserine-containing liposomes on odontogenic differentiation of human dental pulp cells

Park, Hee Chul; Quan, Hongxuan; Yang, Hyeong‐Cheol

doi:10.4012/dmj.2016-127

Cited by 6 publications

(5 citation statements)

References 23 publications

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“…DDM-loaded liposomes showed to be more effective than control liposomes, which can be related to DDM and phosphatidylserine synergism, demonstrating the potential of this formulation in dental restorative processes [ 68 ]. This enhancement of mineralization by phosphatidylserine was also demonstrated by Park and coworkers (2017) when the author analyzed the potential of liposomes composed by phosphatidylserine to stimulate dentin formation in human dental pulp cells [ 96 ].…”

Section: Drug Delivery Systems Used In the Prevention And Treatment O...supporting

confidence: 56%

“…Thus, the advantages of these delivery systems have made them attractive for the treatment of dentistry diseases. Their use have been described for prevention of caries and periodontitis through the disruption of oral biofilms [ 66 , 67 , 92 , 93 , 94 ], reduction in acid erosion [ 95 ], and enhancement of the biomineralization for dental tissue regeneration [ 68 , 96 ].…”

Section: Drug Delivery Systems Used In the Prevention And Treatment O...mentioning

confidence: 99%

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New Technological Approaches for Dental Caries Treatment: From Liquid Crystalline Systems to Nanocarriers

et al. 2023

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Dental caries is the most common oral disease, with high prevalence rates in adolescents and low-income and lower-middle-income countries. This disease originates from acid production by bacteria, leading to demineralization of the dental enamel and the formation of cavities. The treatment of caries remains a global challenge and the development of effective drug delivery systems is a potential strategy. In this context, different drug delivery systems have been investigated to remove oral biofilms and remineralize dental enamel. For a successful application of these systems, it is necessary that they remain adhered to the surfaces of the teeth to allow enough time for the removal of biofilms and enamel remineralization, thus, the use of mucoadhesive systems is highly encouraged. Among the systems used for this purpose, liquid crystalline systems, polymer-based nanoparticles, lipid-based nanoparticles, and inorganic nanoparticles have demonstrated great potential for preventing and treating dental caries through their own antimicrobial and remineralization properties or through delivering drugs. Therefore, the present review addresses the main drug delivery systems investigated in the treatment and prevention of dental caries.

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Section: Drug Delivery Systems Used In the Prevention And Treatment O...supporting

confidence: 56%

Section: Drug Delivery Systems Used In the Prevention And Treatment O...mentioning

confidence: 99%

New Technological Approaches for Dental Caries Treatment: From Liquid Crystalline Systems to Nanocarriers

et al. 2023

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“…The lipid DPPS plays a very important role both for improving AnxA5 incorporation into liposomes and for favoring binding of proteoliposomes to collagens [ 27 ]. Besides that, negative charges can influence the Ca 2+ transport by AnxA5 into DPPS:DPPC:Chol (2:1:1) liposomes, raising the levels of mineral propagation in odontoblast pre-differentiated cells, leading to the odontogenic differentiation [ 48 ]. Therefore, AnxA5 and PS work synergistically both in the Ca 2+ transport to the inside of vesicles and targeting them to collagen fibers in the extracellular matrix.…”

Section: Discussionmentioning

confidence: 99%

Ultrasensitive Diamond Microelectrode Application in the Detection of Ca2+ Transport by AnnexinA5-Containing Nanostructured Liposomes

et al. 2022

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This report describes the innovative application of high sensitivity Boron-doped nanocrystalline diamond microelectrodes for tracking small changes in Ca2+ concentration due to binding to Annexin-A5 inserted into the lipid bilayer of liposomes (proteoliposomes), which could not be assessed using common Ca2+ selective electrodes. Dispensing proteoliposomes to an electrolyte containing 1 mM Ca2+ resulted in a potential jump that decreased with time, reaching the baseline level after ~300 s, suggesting that Ca2+ ions were incorporated into the vesicle compartment and were no longer detected by the microelectrode. This behavior was not observed when liposomes (vesicles without AnxA5) were dispensed in the presence of Ca2+. The ion transport appears Ca2+-selective, since dispensing proteoliposomes in the presence of Mg2+ did not result in potential drop. The experimental conditions were adjusted to ensure an excess of Ca2+, thus confirming that the potential reduction was not only due to the binding of Ca2+ to AnxA5 but to the transfer of ions to the lumen of the proteoliposomes. Ca2+ uptake stopped immediately after the addition of EDTA. Therefore, our data provide evidence of selective Ca2+ transport into the proteoliposomes and support the possible function of AnxA5 as a hydrophilic pore once incorporated into lipid membrane, mediating the mineralization initiation process occurring in matrix vesicles.

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“…After 2 days, cell viability was determined according to a previously established protocol (Park et al . ). Briefly, the cells were washed and replenished with culture media containing 10% 2‐(2‐methoxy‐4‐nitrophenyl)‐3‐(4‐nitrophenyl)5‐(2,4‐disulfophenyl)‐2H‐tetrazolium (Dojindo Laboratories, Kumamoto, Japan) for 1 h at 37°C.…”

Section: Methodsmentioning

confidence: 97%

“…Thereafter, the cells were washed with phosphate-buffered saline (PBS, pH 7.4) and resuspended in fresh culture media in a 96-well plate containing TEGDMA and HEMA at concentrations of 0.5-8.0 mmol L À1 . After 2 days, cell viability was determined according to a previously established protocol (Park et al 2017a). Briefly, the cells were washed and replenished with culture media containing 10% 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)5-(2,4-disulfophenyl)-2H-tetrazolium (Dojindo Laboratories, Kumamoto, Japan) for 1 h at 37°C.…”

Section: Cytotoxicity Assessmentmentioning

confidence: 99%

Effects of triethylene glycol dimethacrylate and hydroxyethyl methacrylate on macrophage polarization

et al. 2019

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Aim To evaluate the effects of hydrophilic dental resin monomers, triethylene glycol dimethacrylate (TEGDMA) and hydroxyethyl methacrylate (HEMA), on the polarization of a human monocyte cell line (THP‐1). Methodology THP‐1 cells were treated with resin monomers at noncytotoxic concentrations for 48 h and were analysed for CD86 and CD206 expressions using flow cytometry. The cells were stimulated for polarization in the presence of resin monomers (co‐treatment) or after treatment with monomers (pre‐treatment). CD86 and CD206 mRNA in co‐treated cells was evaluated using quantitative real‐time polymerase chain reaction. The release of TNF‐α and TGF‐β by pre‐treated and co‐treated cells was assessed using enzyme‐linked immunosorbent assay. Morphological changes of macrophages during polarization were observed using bright‐field microscopy. One‐way analysis of variance was used for statistical analysis. Results TEGDMA (1 mmol L−1) and HEMA (2 mmol L−1) did not induce CD86 and CD206 expressions in THP‐1 cells but rather inhibited their expressions in the co‐treated cells. The inhibitory effects also appeared at the transcription level. However, the expression of surface markers was not affected by pre‐treatment with resin monomers. The release of TNF‐α and TGF‐β by M1‐ and M2‐stimulated cells, respectively, was suppressed by co‐treatment (P < 0.05). Microscopic studies revealed that co‐treatment with resin monomers suppressed polarization‐associated morphological changes such as cell volume increase. Conclusions TEGDMA and HEMA inhibited macrophage polarization to both M1 and M2 at the transcription level, and the inhibitory effects disappeared upon the removal of resin monomers from the cell culture.

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Effects of phosphatidylserine-containing liposomes on odontogenic differentiation of human dental pulp cells

Cited by 6 publications

References 23 publications

New Technological Approaches for Dental Caries Treatment: From Liquid Crystalline Systems to Nanocarriers

New Technological Approaches for Dental Caries Treatment: From Liquid Crystalline Systems to Nanocarriers

Ultrasensitive Diamond Microelectrode Application in the Detection of Ca2+ Transport by AnnexinA5-Containing Nanostructured Liposomes

Effects of triethylene glycol dimethacrylate and hydroxyethyl methacrylate on macrophage polarization

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