Effects of phenolic and protein extracts from Melipona beecheii honey on pathogenic strains of Escherichia coli and Staphylococcus aureus

Ramón-Sierra, Jesús; Martinez-Guevara, Jose; Pool-Yam, Luis; Magaña-Ortíz, Denis; Yam-Puc, Alejandro; Ortiz-Vázquez, Elizabeth

doi:10.1007/s10068-020-00744-4

Cited by 15 publications

(15 citation statements)

References 31 publications

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“…The antimicrobial activity shown by the TPE is similar to that reported by Ramón‐Sierra et al . (2020), where they demonstrated that a protein extract from Melipona beecheii honey inhibited the growth of the pathogens E. coli ATCC 25922 and S. aureus ATCC 25923 with a bactericidal effect at concentrations of 54 and 60 µ g ml −1 , respectively. It is important to take into account that the F1 fraction had 48·7 times less activity than that required with the TPE.…”

Section: Discussionmentioning

confidence: 99%

“…2012; Ramón‐Sierra et al . 2020). The antimicrobial activity of honey has been attributed to different factors, including osmolarity, acidity, compounds of phenolic origin (Kato et al 2012…”

Section: Introductionmentioning

confidence: 99%

“…The studies of the honey proteins mentioned above have been carried out using A. mellifera honey. Since it has been reported that the antimicrobial properties of honey from stingless bees are largely provided by phenolic compounds and proteins (Ramón‐Sierra et al 2020…”

Section: Introductionmentioning

confidence: 99%

“…Apis mellifera honey has proved to be effective against E. coli, Salmonella, Listeria monocytogenes and S. aureus, having been used in the treatment of various illnesses such as diabetic foot ulcers and bacterial conjunctivitis, among others (Boorn et al2010;Queiroz Pimentel et al2013;Morroni et al2018). Specifically, M. beecheii honey has shown an antimicrobial effect against different Gram-positive and Gram-negative pathogenic strains, including E. coli (Chan-Rodriguez et al 2012;Ramón-Sierra et al 2020). The antimicrobial activity of honey has been attributed to different factors, including osmolarity, acidity, compounds of phenolic origin (Kato et al2012), hydrogen peroxide content (Brudzynski 2006) and multi-component polymers as melanoidins (Brudzynski and Miotto 2011).…”

Section: Introductionmentioning

confidence: 99%

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Characterization of a non‐glycosylated fraction from honey proteins of Melipona beecheii with antimicrobial activity against Escherichia coli O157:H7

et al. 2020

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Aims To analyse the non‐glycosylated protein fraction from Melipona beecheii honey for antimicrobial activity against Escherichia coli O157:H7. Methods and Results The proteins from M. beecheii honey were separated according to their degree of glycosylation using Concanavalin A‐affinity chromatography. The total protein extract and its fractions were analysed by 1D and 2D electrophoresis. We also determined the antimicrobial and antihaemolytic activities of the total protein extract and the non‐glycosylated fraction. Furthermore, we evaluated the effect of this non‐glycosylated fraction for the expression of the Stx1, Stx2, EAE and HlyA pathogen genes. Melipona beecheii honey contained at least 24 proteins with molecular weights ranging between 7·6 and 95 kDa and isoelectric points between 3 and 10, three proteins from the 24 are non‐glycosylated. The non‐glycosylated fraction had an MIC90 of 1·128 µg ml−1, and this fraction inhibited the haemolytic activity of the pathogen, as well as reduced the expression of Stx1, Stx2 and HlyA. The MbF1‐2 protein from the non‐glycosylated fraction was sequenced and identified as a homologue of the royal jelly‐like protein of Melipona quadrifasciata. Conclusions The non‐glycosylated protein fraction from M. beecheii honey greatly contributes to antibacterial activity and it is composed of at least three proteins, of which MbF1‐2 provided over 50% of the antimicrobial activity. Significance and Impact of the Study The study showed significant antimicrobial activity from several proteins present in the honey of M. beecheii. Interestingly, the non‐glycosylated protein fraction demonstrated antihaemolytic activity and adversely affected the expression of virulence genes in Escherichia coli O157:H7; these proteins have the potential to be used in developing therapeutic agents against this bacterium.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Characterization of a non‐glycosylated fraction from honey proteins of Melipona beecheii with antimicrobial activity against Escherichia coli O157:H7

et al. 2020

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“…As such, taken orally, in an undiluted form, it can shorten the duration of diarrhea. Honey has also been shown to inhibit the growth of strains such as MRSA and multidrug-resistant P. aeruginosa and Acinetobacter spp., as well as those from the Enterobacteriaceae family that cause burn wound infections and hemolytic uremic syndrome [ 30 , 31 ]. In addition, manuka and capilano honeys have been shown to inhibit the growth of Helicobacter pylori [ 9 ].…”

Section: Antimicrobial Properties Of Bee Productsmentioning

confidence: 99%

Promising Antimicrobial Properties of Bioactive Compounds from Different Honeybee Products

et al. 2021

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Bee products have been known for centuries for their versatile healing properties. In recent decades they have become the subject of documented scientific research. This review aims to present and compare the impact of bee products and their components as antimicrobial agents. Honey, propolis, royal jelly and bee venom are bee products that have antibacterial properties. Sensitivity of bacteria to these products varies considerably between products and varieties of the same product depending on their origin. According to the type of bee product, different degrees of activity were observed against Gram-positive and Gram-negative bacteria, yeasts, molds and dermatophytes, as well as biofilm-forming microorganisms. Pseudomonas aeruginosa turned out to be the most resistant to bee products. An analysis of average minimum inhibitory concentration values for bee products showed that bee venom has the strongest bacterial effectiveness, while royal jelly showed the weakest antibacterial activity. The most challenging problems associated with using bee products for medical purposes are dosage and safety. The complexity and variability in composition of these products raise the need for their standardization before safe and predictable clinical uses can be achieved.

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Effect of conA-unbound proteins from Melipona beecheii honey on the formation of Pseudomonas aeruginosa ATCC 27853 biofilm

Pool-Yam,

Ramón-Sierra,

Oliva

et al. 2024

Arch Microbiol

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Effects of phenolic and protein extracts from Melipona beecheii honey on pathogenic strains of Escherichia coli and Staphylococcus aureus

Cited by 15 publications

References 31 publications

Characterization of a non‐glycosylated fraction from honey proteins of Melipona beecheii with antimicrobial activity against Escherichia coli O157:H7

Characterization of a non‐glycosylated fraction from honey proteins of Melipona beecheii with antimicrobial activity against Escherichia coli O157:H7

Promising Antimicrobial Properties of Bioactive Compounds from Different Honeybee Products

Effect of conA-unbound proteins from Melipona beecheii honey on the formation of Pseudomonas aeruginosa ATCC 27853 biofilm

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