Effects of Phenazepam on the Behavior of C57Bl/6 and BALB/c Mice in the Open Field Test after Naloxone Pretreatment

Середенин, С. Б.; Nadorova, A. V.; Колик, Л. Г.; Yarkova, M. A.

doi:10.1007/s10517-013-2150-0

Cited by 7 publications

(6 citation statements)

References 12 publications

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“…The results of open-field test indicate that GGRE, NS1619, IbTX had no significant effect on the mice's locomotor system. Results of previous studies was similar to our study results (Seredenin et al, 2013). In our study, morphine caused a decrease in mice's motor activity and anxiety, which is similar to the results of a previous study (Wang et al, 2017).…”

Section: Boletín Latinoamericano Y Del Caribe De Plantas Medicinales Y Aromáticas / 480supporting

confidence: 93%

“…In our study, morphine caused a decrease in mice's motor activity and anxiety, which is similar to the results of a previous study (Wang et al, 2017). The present study showed that the effects of the morphine were reversed by naloxone, consistent with the results reported in the literature (Seredenin et al, 2013). Since systemic administration of NS1619 does not readily cross the blood-brain-barrier, NS1619 might not cause anxiety or impair motor activity (Lu et al, 2014).…”

Section: Boletín Latinoamericano Y Del Caribe De Plantas Medicinales Y Aromáticas / 480supporting

confidence: 92%

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Extract of Glycyrrhiza glabraroot attenuates nociception in experimental pain models: The role of BKCa channels

Parlar¹,

Köprülü²,

Arslan³

et al. 2022

BLACPMA

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The aim of this study was to evaluate the functional interaction of Glycyrrhiza glabraroot extract (GGRE) on the large conductance Ca2+-activated K+(BKCa) channels expressed in the peripheral nervous system by using nociception and inflammation models in rodents in vivo. Besides toxicity studies and open field tests, nociception and inflammation tests were performed on rodents. Different doses of GGRE were given orally to rats and mice. Naloxone, indomethacin, morphine, NS1619 and iberiotoxin (IbTX) were administered. GGRE had both anti-nociceptive and anti-inflammatory activity in rats and mice. GGRE exhibited an analgesic effect by decreasing the time-course of the pain threshold or reaction time insome nociceptive tests. Furthermore, GGRE reduced level of pro-inflammatory cytokines, including TNF-α and IL-1β. As a conclusion, GGRE can alleviate the pain sensation of the afferent nerves and can reduce inflammation and associated pain by activating BKCa channels and reducing the levels of TNF-α, IL1β.

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Section: Boletín Latinoamericano Y Del Caribe De Plantas Medicinales Y Aromáticas / 480supporting

confidence: 93%

Section: Boletín Latinoamericano Y Del Caribe De Plantas Medicinales Y Aromáticas / 480supporting

confidence: 92%

Extract of Glycyrrhiza glabraroot attenuates nociception in experimental pain models: The role of BKCa channels

Parlar¹,

Köprülü²,

Arslan³

et al. 2022

BLACPMA

View full text Add to dashboard Cite

show abstract

“…We used a 30-minute interval between drug administration and behavioral testing based on published protocols (Angrini et al, 1998;Birkett et al, 2011;Choleris et al, 2001;Heredia et al, 2013a;Lalonde and Strazielle, 2010;Salomons et al, 2012;Seredenin et al, 1990Seredenin et al, , 2013. Our PK studies determined that peak CDP concentration occurred prior to testing.…”

Section: Discussionmentioning

confidence: 99%

Prototypical anxiolytics do not reduce anxiety-like behavior in the open field in C57BL/6J mice

Thompson

Grabowski-Boase

Tarantino

2015

Pharmacology Biochemistry and Behavior

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Understanding and effectively treating anxiety disorders are a challenge for both scientists and clinicians. Despite a variety of available therapies, the efficacy of current treatments is still not optimal and adverse side effects can result in non-compliance. Animal models have been useful for studying the underlying biology of anxiety and assessing the anxiolytic properties of potential therapeutics. The open field (OF) is a commonly used assay of anxiety-like behavior. The OF was developed and validated in rats and then transferred to use in the mouse with only limited validation. The present study tests the efficacy of prototypical benzodiazepine anxiolytics, chlordiazepoxide (CDP) and diazepam (DZ), for increasing center time in the OF in C57BL/6J (B6) mice. Multiple doses of CDP and DZ did not change time spent in the center of the OF. Increasing illumination in the OF did not alter these results. The non-benzodiazepine anxiolytic, buspirone (BUSP) also failed to increase center time in the OF while the anxiogenic meta-chlorophenylpiperazine (mCPP) increased center time. Additional inbred mouse strains, BALB/cJ (BALB) and DBA/2J (D2) did not show any change in center time in response to CDP. Moreover, evaluation of CDP in B6 mice in the elevated plus maze (EPM), elevated zero maze (EZM) and light dark assay (LD) did not reveal changes in anxiety-like behavior while stress-induced hyperthermia (SIH) was decreased by DZ. Pharmacokinetic (PK) studies suggest that adequate CDP is present to induce anxiolysis. We conclude that the measure of center time in the OF does not show predictive validity for anxiolysis in these inbred mouse strains.

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“…The open field test is a well-known test for behavioral analysis, which has been used for several decades [ 21 , 22 ]. Even though the test is not absolutely specific, it is a good tool to quantify well-being [ 23 , 24 ]. In former analyses, we could already demonstrate the usability of the test to evaluate well-being in PDAC [ 11 , 25 ].…”

Section: Methodsmentioning

confidence: 99%

Genetic Mouse Models to Study Pancreatic Cancer-Induced Pain and Reduction in Well-Being

Hirth

Xie

Höper

et al. 2022

Cells

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In addition to the poor prognosis, excruciating abdominal pain is a major challenge in pancreatic cancer. Neurotropism appears to be the underlying mechanism leading to neuronal invasion. However, there is a lack of animal models suitable for translationally bridging in vitro findings with clinical trials. We characterized KPC (KrasG12D/+; Trp53R172H/+; P48-Cre) and KPPC (KrasG12D/+; Trp53R172H/R172H; P48-Cre) mice with genetically determined pancreatic ductal adenocarcinoma (PDAC) and compared them with an orthotopic pancreatic cancer mouse model, healthy littermates and human tissue. We analyzed behavioral correlates of cancer-associated pain and well-being, and studied neuronal remodeling and cytokine expression. Histologically, we found similarities between KPC and KPPC tissue with human samples. Compared to healthy littermates, we detect nerve fiber hypertrophy, which was not restricted to a certain fiber type. Interestingly, while KPPC mice showed significantly reduced well-being, KPC mice emerged to be better suited for studying long-lasting cancer pain that emerges over a slow course of tumor progression. To address the neuroinflammatory correlate of loss of well-being, we studied cytokine levels in KPPC mice and observed a significant upregulation of CXCL16, TNFRSF5, CCL24, CXCL1, CCL22, CLL20 and CX2CL1. In summary, we demonstrate that the KPC mouse model is best suited to studying cancer pain, whereas the KPPC model can be employed to study cancer-associated reduction in well-being.

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Effects of Phenazepam on the Behavior of C57Bl/6 and BALB/c Mice in the Open Field Test after Naloxone Pretreatment

Cited by 7 publications

References 12 publications

Extract of Glycyrrhiza glabraroot attenuates nociception in experimental pain models: The role of BKCa channels

Extract of Glycyrrhiza glabraroot attenuates nociception in experimental pain models: The role of BKCa channels

Prototypical anxiolytics do not reduce anxiety-like behavior in the open field in C57BL/6J mice

Genetic Mouse Models to Study Pancreatic Cancer-Induced Pain and Reduction in Well-Being

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