Effects of pH on the Antibiotic Resistance of Bacteria Recovered from Diabetic Foot Ulcer Fluid

McArdle, Carla; Lagan, Katie; McDowell, David A.

doi:10.7547/16-033

Cited by 14 publications

(20 citation statements)

References 28 publications

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“…The wound environment of DFUs is significantly more alkaline than acute wounds, contributing to the complex host–microbiome interaction. In a test of different bacterial strains, including Pseudomonas , alkaline pH conditions increased biofilm formation [ 83 ], with pH also exhibiting differential effects on bacterial resistance to antibiotics [ 84 ]. Considering antibiotic resistance testing generally occurs near physiological pH, this difference in bacterial sensitivity should be accounted for when prescribing treatment for an infection found in an abnormally alkaline DFU environment.…”

Section: Pathophysiology Associated With Diabetic Wound Healingmentioning

confidence: 99%

Diabetic Wound-Healing Science

et al. 2021

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Diabetes mellitus is an increasingly prevalent chronic metabolic disease characterized by prolonged hyperglycemia that leads to long-term health consequences. It is estimated that impaired healing of diabetic wounds affects approximately 25% of all patients with diabetes mellitus, often resulting in lower limb amputation, with subsequent high economic and psychosocial costs. The hyperglycemic environment promotes the formation of biofilms and makes diabetic wounds difficult to treat. In this review, we present updates regarding recent advances in our understanding of the pathophysiology of diabetic wounds focusing on impaired angiogenesis, neuropathy, sub-optimal chronic inflammatory response, barrier disruption, and subsequent polymicrobial infection, followed by current and future treatment strategies designed to tackle the various pathologies associated with diabetic wounds. Given the alarming increase in the prevalence of diabetes, and subsequently diabetic wounds, it is imperative that future treatment strategies target multiple causes of impaired healing in diabetic wounds.

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Section: Pathophysiology Associated With Diabetic Wound Healingmentioning

confidence: 99%

Diabetic Wound-Healing Science

et al. 2021

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“…In an in vitro study on DFU samples, McArdle et al showed an increased resistance toward ciprofloxacin for both P. aeruginosa and S. aureus . P. aeruginosa was more resistant under acidic conditions under which alkaline ones were more favorable for the increased resistance of S. aureus [ 123 ].…”

Section: Crosstalk Between Species In Chronic Woundsmentioning

confidence: 99%

Bacterial Interactions in the Context of Chronic Wound Biofilm: A Review

et al. 2022

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Chronic wounds, defined by their resistance to care after four weeks, are a major concern, affecting millions of patients every year. They can be divided into three types of lesions: diabetic foot ulcers (DFU), pressure ulcers (PU), and venous/arterial ulcers. Once established, the classical treatment for chronic wounds includes tissue debridement at regular intervals to decrease biofilm mass constituted by microorganisms physiologically colonizing the wound. This particular niche hosts a dynamic bacterial population constituting the bed of interaction between the various microorganisms. The temporal reshuffle of biofilm relies on an organized architecture. Microbial community turnover is mainly associated with debridement (allowing transitioning from one major representant to another), but also with microbial competition and/or collaboration within wounds. This complex network of species and interactions has the potential, through diversity in antagonist and/or synergistic crosstalk, to accelerate, delay, or worsen wound healing. Understanding these interactions between microorganisms encountered in this clinical situation is essential to improve the management of chronic wounds.

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“…Diabetic wound pH is impacted by these pathophysiological particularities. In contrast to the acidic pH in acute wounds of about 5.5, diabetic wound pH reaches values in the neutral and alkaline range of up to pH 8.5 in patients with diabetes and animal models of diabetes. − The high wound pH is likely related to low-grade chronic inflammation and leakage of physiological fluids, which are at slightly alkaline pH, into the chronic wounds. , …”

Section: Recently Emerged Diagnostic Targetsmentioning

confidence: 99%

“…Bacterial infection elicits an inflammatory response and leads to the upregulation of pro-inflammatory cytokines, ROS, and products of glycolysis such as lactate . Wound pH is also associated with wound healing, as the basic and slightly alkaline pH found in diabetic wounds promotes the growth of pathogenic bacteria. − …”

Section: Recently Emerged Diagnostic Targetsmentioning

confidence: 99%

Next-Generation Diagnostic Wound Dressings for Diabetic Wounds

Stupnitskaia

Matoori

2022

ACS Meas. Sci. Au

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Chronic lower extremity wounds (diabetic foot ulcers) are a serious and prevalent complication of diabetes. These wounds exhibit low healing rates and present a high risk of amputation. Current diagnostic options for foot ulcers are limited to macroscopic wound analysis such as wound depth, implicated tissues, and infection. Molecular diagnostics promises to improve foot ulcer diagnosis, staging, and assessment of the treatment response. In this perspective, we report recent progress in understanding the pathophysiology of diabetic wound healing and point to recently emerged novel molecular targets for wound diagnostics. We discuss selected diagnostic wound dressings under preclinical development that detect one or several inflammatory markers, bacterial secretions, hyperglycemia, and mechanical stress. We also highlight key translational challenges of investigational diagnostic bandages for diabetic foot ulcers.

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Effects of pH on the Antibiotic Resistance of Bacteria Recovered from Diabetic Foot Ulcer Fluid

Cited by 14 publications

References 28 publications

Diabetic Wound-Healing Science

Diabetic Wound-Healing Science

Bacterial Interactions in the Context of Chronic Wound Biofilm: A Review

Next-Generation Diagnostic Wound Dressings for Diabetic Wounds

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