Effects of PFOS, F-53B and OBS on locomotor behaviour, the dopaminergic system and mitochondrial function in developing zebrafish (Danio rerio)

Kalyn, Michael; Lee, Hyojin; Curry, Jory; Tu, Wenqing; Ekker, Marc; Mennigen, Jan A.

doi:10.1016/j.envpol.2023.121479

Cited by 12 publications

(4 citation statements)

References 69 publications

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“…To study PFOS effects on zebrafish larvae, we chose to immerse larvae in media containing PFOS, facilitating quick uptake. Nevertheless, as in similar studies of larval zebrafish behavior (Gaballah et al, 2020;Han et al, 2021;Kalyn et al, 2023;Rericha et al, 2021;Tal & Vogs, 2021;Ulhaq et al, 2013), this approach also necessitated short-term exposures in solutions with PFOS concentrations (2-20 µM) that exceeded typical environmental levels of 0.1 to 600 ng/L (2.0 pM-1.2 nM; Hansen et al, 2002) to achieve significant bioaccumulation. Although environmental and laboratory exposures have inherent differences that must be considered, the concentrations of the same magnitude used in our experiments can exist in the environment because levels as high as 17,000 µg/L (34 µM) have been observed in contaminated water due to spills (Moody et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

“…As such, they provide an excellent experimental framework to study the impacts of PFAS on nervous system function and on complex behaviors. Zebrafish embryos and larvae have been used to assess the impacts of PFAS, including PFOS, on development, immune response, and locomotor behaviors (Gaballah et al, 2020;Han et al, 2021;Kalyn et al, 2023;Paquette et al, 2022;Rericha et al, 2021;Tal & Vogs, 2021;Ulhaq et al, 2013). At early stages, zebrafish skin is highly permeable, and PFAS are readily absorbed (Tal & Vogs, 2021).…”

Section: Introductionmentioning

confidence: 99%

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Perfluorooctane Sulfonate (PFOS) Negatively Impacts Prey Capture Capabilities in Larval Zebrafish

Zoodsma,

Boonkanon,

Running

et al. 2024

Enviro Toxic and Chemistry

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Per‐ and polyfluoroalkyl substances (PFAS) are widely used in many industrial and domestic applications. The wide range use of PFAS has resulted in unintentional human exposures and bioaccumulation in blood and other organs. Perfluorooctanesulfonate (PFOS) is among the most prevalent PFAS in the environment and has been postulated to affect brain functions in exposed organisms. However, the impacts of PFOS in early neural development have not been well‐described. Here, we used zebrafish larvae to assess the effects of PFOS on two fundamental complex behaviors, prey capture and learning. Zebrafish exposed to PFOS concentrations ranging from 2 – 20 µM for differing 48‐hour periods were viable through early larval stages. In addition, PFOS uptake was unaffected by the presence of a chorion. We employed two different experimental paradigms; we first assessed the impacts of increasing organismal PFOS bioaccumulation on prey capture and learning, and second, we probed stage‐specific sensitivity to PFOS by exposing zebrafish at different developmental stages (0‐2 vs 3‐5 days post fertilization). Following both assays we measured the amount of PFOS present in each larva. PFOS levels varied in larvae from different groups within each experimental paradigm. Significant negative correlations were observed between larval PFOS accumulation and percentage of captured prey, while non‐significant negative correlations were observed between PFOS accumulation and experienced‐induced prey capture learning. These findings suggest that PFOS accumulation negatively affects larval zebrafish's ability to perform complicated multisensory behaviors and highlight potential risks of PFOS exposure to animals in the wild, with implications to human health.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Perfluorooctane Sulfonate (PFOS) Negatively Impacts Prey Capture Capabilities in Larval Zebrafish

Zoodsma,

Boonkanon,

Running

et al. 2024

Enviro Toxic and Chemistry

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“…Wang et al [ 50 ] observed that the flame retardant DE-71 impaired the cholinergic system and locomotor activity of zebrafish larvae by dysregulating calcium homeostasis. Moreover, zebrafish locomotion is regulated by the dopaminergic system [ 51 , 52 ], which is known to be disturbed by BDE-47 [ 15 , 41 ]. Indeed, harmed locomotor behavior, given by limited moving capability, can affect the survival of larvae and is a potential ecological threat [ 46 ].…”

Section: Discussionmentioning

confidence: 99%

Comparative Assessment of the Toxicity of Brominated and Halogen-Free Flame Retardants to Zebrafish in Terms of Tail Coiling Activity, Biomarkers, and Locomotor Activity

Rialto,

Marino,

Abe

et al. 2023

Toxics

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BDE-47, a flame retardant that is frequently detected in environmental compartments and human tissues, has been associated with various toxic effects. In turn, information about the effects of aluminum diethyl-phosphinate (ALPI), a halogen-free flame retardant from a newer generation, is limited. This study aims to assess and compare the toxicity of BDE-47 and ALPI to zebrafish by analyzing the tail coiling, locomotor, acetylcholinesterase activities, and oxidative stress biomarkers. At 3000 µg/L BDE-47, the coiling frequency increased at 26–27 h post-fertilization (hpf), but the burst activity (%) and mean burst duration (s) did not change significantly. Here, we considered that the increased coiling frequency is a slight neurotoxic effect because locomotor activity was impaired at 144 hpf and 300 µg/L BDE-47. Moreover, we hypothesized that oxidative stress could be involved in the BDE-47 toxicity mechanisms. In contrast, only at 30,000 µg/L did ALPI increase the catalase activity, while the motor behavior during different developmental stages remained unaffected. On the basis of these findings, BDE-47 is more toxic than ALPI.

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“…Similarly, neurotoxicity was observed for certain PFOS alternatives. For example, perfluorobutane sulfonate (PFBS), a short-chain PFOS alternative, has been shown to cause impairment of visual function in marine medaka ( Oryzias melastigma ) and developmental neurotoxicity in zebrafish ( D. rerio ). , Another PFOS alternative, 6:2 chlorinated polyfluorinated ether sulfonate (Cl-PFESA), can interfere with amino acid neurotransmitter metabolism, disrupt blood-brain barrier formation in the brain of zebrafish ( D. rerio ), and influence their locomotion behavior. , These studies provide evidence that PFOS alternatives, similar to PFOS, have the potential to induce neurotoxicity. However, despite the widespread occurrence of PFECHS in environmental and biological samples in recent years, the potential neurotoxic effects of this PFOS alternative have not been investigated.…”

Section: Introductionmentioning

confidence: 99%

Stereoselective Bioconcentration and Neurotoxicity of Perfluoroethylcyclohexane Sulfonate in Marine Medaka

Wang,

Ruan,

Shao

et al. 2024

Environ. Sci. Technol.

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Perfluoroethylcyclohexane sulfonate (PFECHS) is an emerging per-and polyfluoroalkyl substance used to replace perfluorooctane sulfonate (PFOS), mainly in aircraft hydraulic fluids. However, previous research indicates the potential neurotoxicity of this replacement chemical. In this study, marine medaka (Oryzias melastigma) was exposed to environmentally relevant concentrations of PFECHS (concentrations: 0, 0.08, 0.26, and 0.91 μg/L) from the embryonic stage for 90 days. After exposure, the brain and eyes of the medaka were collected to investigate the bioconcentration potential of PFECHS stereoisomers and their effects on the nervous systems. The determined bioconcentration factors (BCFs) of PFECHS ranged from 324 ± 97 to 435 ± 89 L/ kg and from 454 ± 60 to 576 ± 86 L/kg in the brain and eyes of medaka, respectively. The BCFs of trans-PFECHS were higher than those of cis-PFECHS. PFECHS exposure significantly altered γaminobutyric acid (GABA) levels in the medaka brain and disrupted the GABAergic system, as revealed by proteomics, implying that PFECHS can disturb neural signal transduction like PFOS. PFECHS exposure resulted in significant alterations in multiple proteins associated with eye function in medaka. Abnormal locomotion was observed in PFECHS-exposed medaka larvae, which was rescued by adding exogenous GABA, suggesting the involvement of disrupted GABA signaling pathways in PFECHS neurotoxicity.

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Effects of PFOS, F-53B and OBS on locomotor behaviour, the dopaminergic system and mitochondrial function in developing zebrafish (Danio rerio)

Cited by 12 publications

References 69 publications

Perfluorooctane Sulfonate (PFOS) Negatively Impacts Prey Capture Capabilities in Larval Zebrafish

Perfluorooctane Sulfonate (PFOS) Negatively Impacts Prey Capture Capabilities in Larval Zebrafish

Comparative Assessment of the Toxicity of Brominated and Halogen-Free Flame Retardants to Zebrafish in Terms of Tail Coiling Activity, Biomarkers, and Locomotor Activity

Stereoselective Bioconcentration and Neurotoxicity of Perfluoroethylcyclohexane Sulfonate in Marine Medaka

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