Effects of permethrin exposure on antioxidant enzymes and protein status in Mediterranean clams Ruditapes decussatus

Sellami, Badreddine; Louati, H.; Dellali, Mohamed; Aïssa, Patricia; Mahmoudi, Ebrahim; Coelho, Ana Varela; Sheehan, David

doi:10.1007/s11356-013-2404-4

Cited by 19 publications

(9 citation statements)

References 60 publications

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“…decussatus (Mediterranean clams), suggesting oxidative stress induced in response to permethrin, a type I pyrethroid insecticide, exposure. Proteasome subunit alpha type‐2 (id: 843) also decreased in protein abundance which agreed with previous work in which proteasome subunit alpha type 2 was significantly altered in male mussel gills exposed to BDE 47, a brominated flame‐retardant. Superoxide dismutase (SOD; id: 1072 and 1212) showed increased protein abundance for both spots.…”

Section: Discussionmentioning

confidence: 99%

Redox proteomic analysis of mytilus edulis gills: effects of the pharmaceutical diclofenac on a non‐target organism

Jaafar

Coelho

Sheehan

2015

Drug Testing and Analysis

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Veterinary and human pharmaceuticals are an emerging category of chemical pollutants with potential to cause serious toxicity to non-target organisms. Filter-feeding aquatic organisms such as mussels are especially threatened. In this study, the blue mussel, Mytilus edulis, was exposed to two doses (0.2 mg/L and 1 mg/L) of the anti-inflammatory diclofenac. Effects on the gill, the principal feeding organ of mussels, were investigated. It was noted that, while no effect was evident on gill glutathione transferase or catalase activities, there was a tissue-specific increase in glutathione reductase activity and reduction in total protein thiol groups. Two dimensional electrophoresis was performed and some affected proteins identified by in-gel tryptic digestion and peptide mass fingerprinting. Of these, four unique proteins (caspase 3/7-4, heat-shock cognate protein 70, a predicted enolase-like protein, arginine kinase) were found to be oxidized whilst eight unique proteins (β-tubulin, actin, isocitrate dehydrogenase, arginine kinase, heavy metal-binding HIP, cytosolic malate dehydrogenase, proteasome subunit alpha type 2, Mg: bb02e05 (glyceraldehyde-3-phosphate dehydrogenase) and superoxide dismutase) were found to have altered abundance. In addition, bioinformatic analysis suggested putative identities for six hypothetical proteins which either were oxidized or decreased in abundance. These were; 78 kDa glucose-regulated protein precursor, α-enolase, calreticulin, mitochondrial H + -ATPase, palmitoyl protein thioesterase 1 and initiation factor 5a. It is concluded that diclofenac causes significant oxidative stress to gills and that this affects key structural, metabolic and stress-response proteins.

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Section: Discussionmentioning

confidence: 99%

Redox proteomic analysis of mytilus edulis gills: effects of the pharmaceutical diclofenac on a non‐target organism

Jaafar

Coelho

Sheehan

2015

Drug Testing and Analysis

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“…7 Despite this apparently paradoxical result, our results are consistent with those observed in several bivalve species exposed to contaminants. 43,44 Oysters exposed to isoproturon 10X showed higher CAT activity than controls. This result agrees well with the fact that the increase in SOD activity in these animals should enhance H 2 O 2 .…”

Section: Energy Metabolismmentioning

confidence: 93%

Energy and Antioxidant Responses of Pacific Oyster Exposed to Trace Levels of Pesticides

Epelboin

Quéré

Pichereau

et al. 2015

Chem. Res. Toxicol.

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Here, we assess the physiological effects induced by environmental concentrations of pesticides in Pacific oyster Crassostrea gigas. Oysters were exposed for 14 d to trace levels of metconazole (0.2 and 2 μg/L), isoproturon (0.1 and 1 μg/L), or both in a mixture (0.2 and 0.1 μg/L, respectively). Exposure to trace levels of pesticides had no effect on the filtration rate, growth, and energy reserves of oysters. However, oysters exposed to metconazole and isoproturon showed an overactivation of the sensing-kinase AMP-activated protein kinase α (AMPKα), a key enzyme involved in energy metabolism and more particularly glycolysis. In the meantime, these exposed oysters showed a decrease in hexokinase and pyruvate kinase activities, whereas 2-DE proteomic revealed that fructose-1,6-bisphosphatase (F-1,6-BP), a key enzyme of gluconeogenesis, was up-regulated. Activities of antioxidant enzymes were higher in oysters exposed to the highest pesticide concentrations. Both pesticides enhanced the superoxide dismutase activity of oysters. Isoproturon enhanced catalase activity, and metconazole enhanced peroxiredoxin activity. Overall, our results show that environmental concentrations of metconazole or isoproturon induced subtle changes in the energy and antioxidant metabolisms of oysters.

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“…Los biomarcadores anteriormente mencionados se han empleado en el análisis ecotoxicológico en diferentes bivalvos, como en las almejas Tapes philippinarum (11) Anodonta cygnea (21) , Scrobicularia plana (28) , Ruditapes decussatus (46)(47)(48)75,82) ,…”

Section: Antecedentesunclassified

“…También, es importante tomar en cuenta que la relación detectada entre la temperatura del agua y la respuesta antioxidante en las branquias probablemente esté relacionada con el hecho de que este tejido está en contacto directo con el agua, siendo más sensible a fluctuaciones en parámetros ambientales y más propenso a estrés oxidativo que otros tejidos (85) . sterna en La Marina en las dos épocas de muestreo (verano e invierno) (46) .…”

Section: Comportamiento De Actividad Acetilcolinesterasa En éPoca De unclassified

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Determinación del estado de salud de dos poblaciones de Pteria sterna presentes en sitios con diferente grado de contaminación mediante la evaluación de biomarcadores bioquímicos y genéticos

Eslava

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Effects of permethrin exposure on antioxidant enzymes and protein status in Mediterranean clams Ruditapes decussatus

Cited by 19 publications

References 60 publications

Redox proteomic analysis of mytilus edulis gills: effects of the pharmaceutical diclofenac on a non‐target organism

Redox proteomic analysis of mytilus edulis gills: effects of the pharmaceutical diclofenac on a non‐target organism

Energy and Antioxidant Responses of Pacific Oyster Exposed to Trace Levels of Pesticides

Determinación del estado de salud de dos poblaciones de Pteria sterna presentes en sitios con diferente grado de contaminación mediante la evaluación de biomarcadores bioquímicos y genéticos

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