Effects of perioperative hypothermia on healing of anastomosis of the colon in rats

Oliveira, João Carlos Costa de; Oliveira, Camila Helena de; Oliveira, Henrique Eduardo; Colombeli, Gianfranco Luigi; Heck, Nicoli De Bona; Pereira, Aline Neves; d'Acâmpora, Armando José

doi:10.1007/s00384-013-1695-8

Cited by 8 publications

(5 citation statements)

References 23 publications

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“…We could reproduce the results of de Oliveira et al [ 24 ] where increased IGF-1 mRNA levels were observed in the anastomotic wounds compared with the noninjured colon, emphasizing the importance of IGF-1 in anastomotic wound healing. ZP1849 treatment stimulated an early increase in IGF-1 expression in the noninjured colon, and IGF-1 mRNA levels were further increased in the anastomotic wounds on day 5 compared to vehicle.…”

Section: Discussionsupporting

confidence: 85%

Glucagon-Like Peptide-2 Analogue ZP1849 Augments Colonic Anastomotic Wound Healing

Kjær

Russell

Schjerling

et al. 2020

Gastroenterology Research and Practice

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Background. The enteroendocrine hormone glucagon-like peptide- (GLP-) 2 is a potent trophic factor in the gastrointestinal tract. The GLP-2 receptor (GLP-2R) is expressed in the stroma of the large bowel wall, which is the major therapeutic target area to prevent anastomotic leakage. We investigated the efficacy of the long-acting GLP-2 analogue ZP1849 on colonic anastomotic wound healing. Methods. Eighty-seven male Wistar rats were stratified into four groups and received daily treatment with vehicle or ZP1849 starting one day before (day -1) end-to-end anastomosis was constructed in the left colon on day 0, and on days 0 (resected colon segment), 3, and 5, gene expressions of GLP-2R, Ki67, insulin-like growth factor- (IGF-) 1, type I (COL1A1) and type III (COL3A1) procollagens, cyclooxygenase- (COX-) 1, COX-2, and matrix metalloproteinase- (MMP-) 7 were quantified by RT-qPCR. Breaking strength, myeloperoxidase (MPO), transforming growth factor- (TGF-) β1, and soluble collagen proteins were measured on days 3 and 5. Results. ZP1849 treatment increased Ki67 (P<0.0001) and IGF-1 (P<0.05) mRNA levels in noninjured colon day 0, and postoperatively in the anastomotic wounds compared to vehicle-treated rats. ZP1849-treated rats had increased (P=0.042) anastomotic breaking strength at day 5 compared with vehicle. COL1A1 and COL3A1 mRNA levels (P<0.0001) and soluble collagen proteins (P<0.05) increased from day 3 to day 5 in ZP1849-treated rats, but not in vehicle-treated rats. COX-2 mRNA and MPO protein levels decreased from day 3 to day 5 (P<0.001) in both groups. ZP1849 treatment reduced TGF-β1 protein levels on day 5 (P<0.001) but did not impact MMP-7 transcription. Conclusions. The GLP-2 analogue ZP1849 increased breaking strength, IGF-1 expression, and cell proliferation, which may be beneficial for colonic anastomotic wound healing.

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Section: Discussionsupporting

confidence: 85%

Glucagon-Like Peptide-2 Analogue ZP1849 Augments Colonic Anastomotic Wound Healing

Kjær

Russell

Schjerling

et al. 2020

Gastroenterology Research and Practice

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show abstract

“…33 Wound healing is delayed in hypothermia due to decreased inflammatory infiltrates, reduced growth factor production, and less collagen deposition. 34 Hypothermia can even enhance the ability of cancer cells to proliferate. 35 The clotting system can be profoundly affected by hypothermia.…”

Section: Deleterious Effects Of Hypothermiamentioning

confidence: 98%

Inadvertent Perianesthetic Hypothermia in Small Animal Patients

Clark‐Price

2015

Veterinary Clinics of North America: Small Animal Practice

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“…Oliveira et al 30,31 reported a detrimental effect of perioperative hypothermia on the colonic anastomotic healing in animal models with decrease of inflammatory cytokines (IL-1, IL-6, IL-10) and growth factors (IGF-1, VEGF) in the seventh and fourteenth postoperative days.…”

mentioning

confidence: 99%

Ghrelin and gastrointestinal wound healing. A new perspective for colorectal surgery

et al. 2018

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Effects of perioperative hypothermia on healing of anastomosis of the colon in rats

Abstract: Perioperative hypothermia had detrimental effects on the healing of colonic anastomosis in rats.

Cited by 8 publications

References 23 publications

Glucagon-Like Peptide-2 Analogue ZP1849 Augments Colonic Anastomotic Wound Healing

Glucagon-Like Peptide-2 Analogue ZP1849 Augments Colonic Anastomotic Wound Healing

Inadvertent Perianesthetic Hypothermia in Small Animal Patients

Ghrelin and gastrointestinal wound healing. A new perspective for colorectal surgery

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