Effects of perinatal protein deprivation and recovery on esophageal myenteric plexus

Greggio, Flávio Marino; Fontes, Ricardo Bragança de Vasconcellos; Maifrino, Laura Beatriz Mesiano; Castelucci, Patrícia; Souza, Romeu Rodrigues de; Liberti, Édson Aparecido

doi:10.3748/wjg.v16.i5.563

Cited by 20 publications

(23 citation statements)

References 26 publications

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“…Several authors have worked with similar experimental protocols to the one used in the present study; however, they used 42 day-old animals (CASTELUCCI et al, 2002;GOMES et al, 2006;GREGGIO et al, 2010;LIBERTI et al, 2007;OLIVEIRA et al, 2010). Since there are no indications in these studies of animals being monitored in the period evaluated (60 days), nor of any metabolic monitoring.…”

Section: Discussionmentioning

confidence: 99%

“…These data were obtained through the morphoquantitative characterization of the myenteric plexus of undernourished rats (CASTELUCCI et al, 2002;GOMES et al, 2006;GREGGIO et al, 2010). Based on these studies and since the animals in the U group suffered protein deprivation, it is possible that the digestive system of these animals did not exhibit severe changes.…”

Section: Discussionmentioning

confidence: 99%

“…The experimental protocol was performed with adaptations from previously published work by our group (GREGGIO et al, 2010;LIBERTI et al, 2007;OLIVEIRA et al, 2010). Wistar rats (Rattus norvegicus) were used (23 males and 23 females, between 90 and 120 d of age with body masses between 280 and 320 g), obtained from the animal colony of the Biomedical Science Institute, USP.…”

Section: Animals and Experimental Groupsmentioning

confidence: 99%

“…Several studies have been published on the effects of protein undernourishment and the role the renourishment plays in various organs and tissues (BAPTISTA JS et al, 2013;GREGGIO et al, 2010;LIBERTI et al, 2007;OLIVEIRA et al, 2010). However, the morphological and structural changes caused by this state of protein deprivation did not reveal or bring understanding to the metabolic aspects of the adopted experimental model.…”

Section: Introductionmentioning

confidence: 99%

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Metabolic effects of early protein undernourishment and renourishment in wistar rats

Baptista¹,

Cavalli²,

Luiz³

et al. 2015

Biosci. J.

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ABSTRACT:Malnutrition remains an important public health condition by affecting mostly children. The present study aimed to examine the effects of prenatal protein undernourishment and postnatal renourishment on the metabolism of pre-adult animals. Forty-five male Wistar rats originating from different litters were used. The Nourished (N, n=15) and Undernourished (U, n=30) groups were maintained on proteic and hypoproteic diets (20% and 5% casein, respectively) from the intrauterine phase until the end of the experiment. The Renourished group (R) contained 15 animals, randomly chosen from U group, that consumed the N diet beginning on day 21 (weaning). All groups were maintained until day 60. The experimental data was acquired daily for body weight, feed and water intake, feces and urine excretion. Data analysis was made with the total results and the ratio between them. The U group animals developed uniformly sparse hair late and experienced intense sweating, features that were not observed during the experiment in the R and N animals groups. The R group had the largest weight gain and feed and water intake ratio/weight at experimental period, followed by the N group animals. The final body weight and food intake decreased gradually in the order N-R-U, but none of the other parameters showed a statistical difference between the N and R animals. Compared to groups N and R, the U group animals consumed 90% and 81% more food and water, respectively. Protein undernourishment induced metabolic dysfunction in the U group animals. The similarity in the experimental parameters of the N and R groups suggests that an early feeding correction was effective in reestablishing the organic functions of the R group animals.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Animals and Experimental Groupsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Metabolic effects of early protein undernourishment and renourishment in wistar rats

Baptista¹,

Cavalli²,

Luiz³

et al. 2015

Biosci. J.

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show abstract

“…The myenteric plexus as an incompact and irregular plexo-ganglionic network with different expression of AChE activity was observed in the wild mouse (Calomys callosus), hamster, rat and guinea pig. In these species the wall of the oesophagus contained striated muscles [16][17][18][19]. A similar level of development of the Auerbach plexus was found in chinchilla.…”

Section: Discussionmentioning

confidence: 49%

Organization of the innervation of the oesophagus and stomach in chinchilla (Chinchilla laniger, Molina)

Nowak

2013

Folia Histochem Cytobiol.

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Using histochemical and histological techniques the morphology and topography of the autonomic plexuses of the oesophagus and stomach in chinchilla (Chinchilla laniger) were investigated. Myenteric and submucosal plexuses were stained for cholinergic and adrenergic structures. Our results showed that the cholinergic structures were formed by networks of various shape and of different size of the neuron agglomerates and nerve fibres. Adrenergic fibres were also found. Density of these structures varied in different parts of the investigated organs. This study is the first attempt to localize the intramural autonomic plexuses in chinchilla.

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Differential effects of undernourishment on the differentiation and maturation of rat enteric neurons

et al. 2013

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The colocalization, number, and size of various classes of enteric neurons immunoreactive (IR) for the purinergic P2X2 and P2X7 receptors (P2X2R, P2X7R) were analyzed in the myenteric and submucosal plexuses of control, undernourished, and re-fed rats. Pregnant rats were exposed to undernourishment (protein-deprivation) or fed a control diet, and their offspring comprised the following experimental groups: rats exposed to a normal diet throughout gestation until postnatal day (P)42, rats protein-deprived throughout gestation and until P42, and rats protein-deprived throughout gestation until P21 and then given a normal diet until P42. Immunohistochemistry was performed on the myenteric and submucosal plexuses to evaluate immunoreactivity for P2X2R, P2X7R, nitric oxide synthase (NOS), choline acetyltransferase (ChAT), calbindin, and calretinin. Double-immunohistochemistry of the myenteric and submucosal plexuses demonstrated that 100% of NOS-IR, calbindin-IR, calretinin-IR, and ChAT-IR neurons in all groups also expressed P2X2R and P2X7R. Neuronal density increased in the myenteric and submucosal plexuses of undernourished rats compared with controls. The average size (profile area) of some types of neurons in the myenteric and submucosal plexuses was smaller in the undernourished than in the control animals. These changes appeared to be reversible, as animals initially undernourished but then fed a normal diet at P21 (re-feeding) were similar to controls. Thus, P2X2R and P2X7R are present in NOS-positive inhibitory neurons, calbindin- and calretinin-positive intrinsic primary afferent neurons, cholinergic secretomotor neurons, and vasomotor neurons in rats. Alterations in these neurons during undernourishment are reversible following re-feeding.

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Effects of perinatal protein deprivation and recovery on esophageal myenteric plexus

Abstract: Protein deprivation causes a delay in neuronal maturation but postnatal recovery can almost completely restore the normal morphology of myenteric neurons.

Cited by 20 publications

References 26 publications

Metabolic effects of early protein undernourishment and renourishment in wistar rats

Metabolic effects of early protein undernourishment and renourishment in wistar rats

Organization of the innervation of the oesophagus and stomach in chinchilla (Chinchilla laniger, Molina)

Differential effects of undernourishment on the differentiation and maturation of rat enteric neurons

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