Effects of perfusate leucine concentration on the metabolism of valine by the isolated rat hindquarter

Zapalowski, Carol; Miller, Robert H.; Dixon, John R.; Harper, Alfred E.

doi:10.1016/0026-0495(84)90246-4

Cited by 19 publications

(2 citation statements)

References 29 publications

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“…However, we provided evidence that the leucine loading into the coronary artery did not increase myocardial intracellular free leucine concentration in piglets undergoing ECMO. Consistent with prior studies (40), leucine infusion did decrease concentrations for both valine and isoleucine. Because concentrations for the other amino acids did not decrease, the data conform with previous contention that leucine promotes oxidative degradation of these other branched chain amino acids.…”

Section: Discussionsupporting

confidence: 88%

Myocardial oxidative metabolism and protein synthesis during mechanical circulatory support by extracorporeal membrane oxygenation

Priddy

Kajimoto

Ledee

et al. 2013

American Journal of Physiology-Heart and Circulatory Physiology

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Extracorporeal membrane oxygenation (ECMO) provides essential mechanical circulatory support necessary for survival in infants and children with acute cardiac decompensation. However, ECMO also causes metabolic disturbances, which contribute to total body wasting and protein loss. Cardiac stunning can also occur, which prevents ECMO weaning, and contributes to high mortality. The heart may specifically undergo metabolic impairments, which influence functional recovery. We tested the hypothesis that ECMO alters oxidative metabolism and protein synthesis. We focused on the amino acid leucine and integration with myocardial protein synthesis. We used a translational immature swine model in which we assessed in heart 1) the fractional contribution of leucine (FcLeucine) and pyruvate to mitochondrial acetyl-CoA formation by nuclear magnetic resonance and 2) global protein fractional synthesis (FSR) by gas chromatography-mass spectrometry. Immature mixed breed Yorkshire male piglets (n = 22) were divided into four groups based on loading status (8 h of normal circulation or ECMO) and intracoronary infusion [(13)C(6),(15)N]-L-leucine (3.7 mM) alone or with [2-(13)C]-pyruvate (7.4 mM). ECMO decreased pulse pressure and correspondingly lowered myocardial oxygen consumption (∼40%, n = 5), indicating decreased overall mitochondrial oxidative metabolism. However, FcLeucine was maintained and myocardial protein FSR was marginally increased. Pyruvate addition decreased tissue leucine enrichment, FcLeucine, and Fc for endogenous substrates as well as protein FSR. The heart under ECMO shows reduced oxidative metabolism of substrates, including amino acids, while maintaining 1) metabolic flexibility indicated by ability to respond to pyruvate and 2) a normal or increased capacity for global protein synthesis.

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Section: Discussionsupporting

confidence: 88%

Myocardial oxidative metabolism and protein synthesis during mechanical circulatory support by extracorporeal membrane oxygenation

Priddy

Kajimoto

Ledee

et al. 2013

American Journal of Physiology-Heart and Circulatory Physiology

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show abstract

“…The preparation was then placed in the NMR tube. At time 0 of the experiment, the perfusion medium was replaced by a Krebs-Henseleit buffer containing 3.5% bovine serum albumin, 20% fresh ovine red cells, all amino acids from plasma at physiological levels [21], 0.7 mmol/L papaverine chloride and [ 12 C] glucose (10 mmol/L) for 20 min. The tube was quickly placed in the magnet.…”

Section: Perfusion Experimentsmentioning

confidence: 99%

Insulin-dependent glycogen synthesis is delayed in onset in the skeletal muscle of food-deprived aged rats

Meynial-Denis

Miri

Biélicki³

et al. 2005

The Journal of Nutritional Biochemistry

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The metabolism and metabolic effects of ketoacids

May

1989

Diabetes Metab. Rev.

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Effects of perfusate leucine concentration on the metabolism of valine by the isolated rat hindquarter

Cited by 19 publications

References 29 publications

Myocardial oxidative metabolism and protein synthesis during mechanical circulatory support by extracorporeal membrane oxygenation

Myocardial oxidative metabolism and protein synthesis during mechanical circulatory support by extracorporeal membrane oxygenation

Insulin-dependent glycogen synthesis is delayed in onset in the skeletal muscle of food-deprived aged rats

The metabolism and metabolic effects of ketoacids

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