Effects of percutaneous needle liver biopsy on dairy cow behaviour

Mølgaard, Lene; Damgaard, Birthe Marie; Bjerre-Harpøth, Vibeke; Herskin, Mette S.

doi:10.1016/j.rvsc.2012.04.001

Cited by 41 publications

(35 citation statements)

References 33 publications

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“…Despite the effort to simulate all manipulations foreseen for the challenge day on the control day, the presence of more people and equipment in the barn might have provoked a higher degree of disturbance to the cows, independently from the treatment group. Moreover, as udder and liver biopsies were taken at the end of the control day, it cannot be excluded that residual discomfort in the affected area might have prevented the cows from lying down [23]. For time spent lying contrasting results have been previously reported in cows affected by LPS-induced mastitis: while in some studies a clear reduction of the time spent lying was detected after the intramammary challenge [4, 6], in others no differences were found [24].…”

Section: Discussionmentioning

confidence: 99%

Experimentally induced subclinical mastitis: are lipopolysaccharide and lipoteichoic acid eliciting similar pain responses?

et al. 2017

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BackgroundPain accompanying mastitis has gained attention recently as a relevant welfare compromising aspect of disease. Adequate pain recognition and therapy are necessary in the context of a modern and ethically acceptable dairy care. For research purposes mastitis is often induced by intramammary infusion of immunogenic bacterial cell wall components. Lipopolysaccharide (LPS) from Escherichia coli and lipoteichoic acid (LTA) from Staphylococcus aureus are commonly administered to this end. While the immune response to specific immunogenic components has been well characterized, not much is known about their role on the expression of pain indicators. The aim of this study was to trial the effects of an intramammary challenge of LTA or LPS on the degree of pain and discomfort as indicated by both physiological and behavioral variables in cows. The hypothesis was that a similar degree of pain can be identified in LTA as well as in LPS induced mastitis.ResultsOn the challenge day, compared to pre-challenge, total pain index increased for all treatment groups (LPS; LTA and control), the LPS group having significantly higher values than the control group (P = 0.01). Similarly, pain visual analogue scale (VAS) increased significantly in all cows following treatment on the challenge day. Furthermore, compared to baseline, higher VAS were found 3, 4 and 5 h after the challenge in cows of the LPS group (P3h, 4h < 0.001 and P5h = 0.001) and 7 h after the challenge in cows of the LTA group (P7h = 0.002). In the control group, VAS was higher 5 h after the challenge (P5h = 0.001). On the challenge day, udder edema was higher in the LPS than in the control group (P = 0.007). Furthermore, 4 h after the challenge, milk cortisol was significantly higher than at baseline in the LPS group (P < 0.001).ConclusionsWhen administered at equipotent doses targeting a standard somatic cell count increase, intramammary LPS seems to be accompanied by a higher degree of pain and discomfort than LTA, as suggested by the modifications of the outcome variables total pain index, VAS, udder edema and milk cortisol.Electronic supplementary materialThe online version of this article (doi:10.1186/s13028-017-0306-z) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Experimentally induced subclinical mastitis: are lipopolysaccharide and lipoteichoic acid eliciting similar pain responses?

et al. 2017

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“…The adverse effects of illness on eating and rumination as well as the ameliorating effects of treatment are of primary clinical interest. It was shown that experimental intramammary challenge with Escherichia coli caused a decrease in rumination activity within 24 hours (Fogsgaard et al, 2012), and collection of a liver biopsy sample also led to reduced rumination, most likely because of pain (Mølgaard et al, 2012). Cows with left displacement of the abomasum (LDA) had significantly lower rumination activity, particularly fewer chewing cycles per regurgitated bolus, for five days after successful surgical correction, whereas eating variables did not differ between operated and healthy cows (Trösch, 2013;Braun et al, 2015c).…”

Section: Introductionmentioning

confidence: 99%

Eating and rumination behaviour in cows with traumatic reticuloperitonitis

Braun

Tschoner

Hässig

et al. 2017

SAT

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Eating and rumination behaviour in 22 cows with traumatic reticuloperitonitis was recorded after the start of treatment. Based on the results of clinical, ultrasonographic and radiographic examinations, the cows were divided into two groups: Group A consisted of 12 cows without an intramural or perforating foreign body and group B had 10 cows with an intramural or perforating foreign body. Cows of group A received amoxicillin, flunixin meglumine and a NaCl-glucose infusion, and cows of group B received the same treatment accompanied by foreign body removal via ruminotomy. All cows were fitted with a horse halter equipped with a pressure sensor integrated into the noseband, and eating and rumination activities were recorded continuously for 7 days. Additional 24-hour-recording periods occurred on days 14 and 30. Results obtained previously from 300 healthy cows were used for comparison. Median daily eating time in group A was 168 minutes (reference interval 211 to 319 min) on day 1 and increased to within the reference interval by day 3. Eating time on day 14 was 290 minutes and significantly longer than on day 1. Eating time was 294 minutes on day 30. Eating time in group B was comparable, and the patterns of normalisation for the number of chewing cycles related to eating, rumination time, the number of regurgitated cuds per day and the number of chewing cycles per cud were similar to that of group A: all variables were significantly reduced on day 1 and normalised within 3 to 5 days. The findings showed that eating and rumination are reduced in cows with traumatic reticuloperitonitis and that successful treatment results in rapid normalisation. Monitoring eating and rumination behaviour using a pressure sensor integrated into a halter allows objective clinical assessment of cows recovering from traumatic reticuloperitonitis. SummaryEating and rumination behaviour in 22 cows with traumatic reticuloperitonitis was recorded after the start of treatment. Based on the results of clinical, ultrasonographic and radiographic examinations, the cows were divided into two groups: Group A consisted of 12 cows without an intramural or perforating foreign body and group B had 10 cows with an intramural or perforating foreign body. Cows of group A received amoxicillin, flunixin meglumine and a NaCl-glucose infusion, and cows of group B received the same treatment accompanied by foreign body removal via ruminotomy. All cows were fitted with a horse halter equipped with a pressure sensor integrated into the noseband, and eating and rumination activities were recorded continuously for 7 days. Additional 24-hour-recording periods occurred on days 14 and 30. Results obtained previously from 300 healthy cows were used for comparison. Median daily eating time in group A was 168 minutes (reference interval 211 to 319 min) on day 1 and increased to within the reference interval by day 3. Eating time on day 14 was 290 minutes and significantly longer than on day 1. Eating time was 294 minutes on day 30. Eating time in group...

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“…For instance, in cattle, following a percutaneous needle liver biopsy (under local anesthesia), they presented increased restlessness, head shaking, and decreased rumination, which possibly indicated pain (Mølgaard et al, 2012). It is important to highlight that in the current study no behavioral changes were identified.…”

Section: Discussionmentioning

confidence: 61%

“…In goats, there are few reports that describe biopsy techniques for soft tissues (Duarte et al, 2009;Kassem et al, 2011). In ruminants, most of the studies have been conducted on variations of the percutaneous biopsy technique (Harvey et al, 1984;Ferreira et al, 1996;Mølgaard et al, 2012). However, US (Lofstedt et al, 1988;Néspoli et al, 2010) or LP biopsies (Phillippo, 1973;Duarte et al, 2009;Kassem et al, 2011) have been quite unexplored, even with their recognized accuracy and safety.…”

Section: Discussionmentioning

confidence: 99%

Comparative analysis of laparoscopic and ultrasound-guided biopsy methods for gene expression analysis in transgenic goats

et al. 2015

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ABSTRACT. The present study aimed to compare laparoscopic (LP) and ultrasound-guided (US) biopsy methods to obtain either liver or splenic tissue samples for ectopic gene expression analysis in transgenic goats. Tissue samples were collected from human granulocyte colony stimulating factor (hG-CSF)-transgenic bucks and submitted to realtime PCR for the endogenous genes (Sp1, Baff, and Gapdh) and the transgene (hG-CSF). Both LP and US biopsy methods were successful in obtaining liver and splenic samples that could be analyzed by PCR (i.e., sufficient sample sizes and RNA yield were obtained). Although the number of attempts made to obtain the tissue samples was similar (P > 0.05), LP procedures took considerably longer than the US method (P = 0.03). Finally, transgene transcripts were not detected in spleen or liver 8673 Biopsy methods for ectopic expression in transgenic bucks ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (3): 8672-8684 (2015) samples. Thus, for the phenotypic characterization of a transgenic goat line, investigation of ectopic gene expression can be made successfully by LP or US biopsy, avoiding the traditional approach of euthanasia.

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Effects of percutaneous needle liver biopsy on dairy cow behaviour

Cited by 41 publications

References 33 publications

Experimentally induced subclinical mastitis: are lipopolysaccharide and lipoteichoic acid eliciting similar pain responses?

Experimentally induced subclinical mastitis: are lipopolysaccharide and lipoteichoic acid eliciting similar pain responses?

Eating and rumination behaviour in cows with traumatic reticuloperitonitis

Comparative analysis of laparoscopic and ultrasound-guided biopsy methods for gene expression analysis in transgenic goats

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