Effects of peptide Pro-Gly-Pro-Leu under conditions of normal hemostasis and thrombus formation in rats

Abstract

Tetrapeptide Pro-Gly-Pro-Leu in vitro effectively inhibited platelet aggregation over the entire range of studied concentrations (10(-12)-10(-3) M). In concentrations of 10(-9)-10(-3) M it exhibits fibrinolytic activity and in concentrations of 10(-5)-10(-3) M has anticoagulant properties. Under in vivo conditions the analyzed tetrapeptide in a dose of 1 mg/kg increased anticoagulant, total and fibrin depolymerizating activities and increased activity of plasminogen activator. Intravenous administration produced more pronounced anticoagulant effect and leads to a greater increase in activity of plasminogen activator than intranasal administration. Tetrapeptide Pro-Gly-Pro-Leu also exerts antithrombotic effect. Preliminary repeated intranasal administration of the peptide before blood clot formation reduces the weight of fresh fibrin clots.

Synthetic corticotropins and the GABA‐receptor system: Direct and delayed effects

“…ACTH(6‐9)PGP enhanced cell viability in the H 2 O 2 , tBH, and KCN cytotoxicity models (Akimov et al, 2021), significantly increased the number of survived cultured cortical neurons in the model of glutamate excitotoxicity (Bakaeva et al, 2020), showed an anxiolytic effect in chronic immobilization stress model (Vorvul et al, 2022). Tetrapeptide PGPL affected blood hemodynamics (Myasoedov et al, 2016; Obergan et al, 2011), reduced the activity of apoptosis (caused by “social” stress) and decreased the levels of caspase‐3 and caspase‐8 in the blood serum of white rats (Yasenyavskaya et al, 2021).…”