Effects of pentoxifylline treatment before freezing on motility, viability and acrosome status of poor quality human spermatozoa cryopreserved by the liquid nitrogen vapor method

Esteves, Sandro C.; Spaine, D.M.; Cedenho, A.P.

doi:10.1590/s0100-879x2006005000118

Cited by 45 publications

(48 citation statements)

References 26 publications

(47 reference statements)

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“…Moreover, mean pre-freeze sperm motility was 48.6% in our group when compared to 73.4% in theirs. Given that sperm cryosurvival is partially dependent on the semen quality before freezing (13,25,26), we speculate that sperm produced by men with severely defective spermatogenesis is more sensitive to cryoinjury than their normal counterpart is.…”

Section: Normozoospermicmentioning

confidence: 96%

Resistance of human spermatozoa to cryoinjury in repeated cycles of thaw-refreezing

Verza¹,

Feijó²,

Esteves³

2009

Int. braz j urol.

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Objective: To study the resistance of human spermatozoa to cryoinjury in repeated cycles of thaw-refreezing by using the fast liquid nitrogen vapor method. Materials and Methods: Semen specimens were obtained from sixteen normal and oligozoospermic individuals who required disposal at the sperm bank. Five of them had testicular cancer. Specimens were thawed and an aliquot was removed for analysis. The remaining specimens were refrozen without removing the cryomedia. Repeated freeze-thaw cycles were performed until no motile sperm were observed. Sperm motility, number of motile spermatozoa and viability were determined after thawing. Resistance to cryoinjury was compared between groups and also after each refreezing cycle within groups. Results: Motile spermatozoa were recovered after five and two refreeze-thawing cycles in normozoospermic and oligozoospermic specimens, respectively. There were no significant differences in the recovery of motile spermatozoa between thaws within each group of normal and oligozoospermic specimens, but percentage motility and total number of motile spermatozoa were significantly lower in the oligozoospermic one. Specimens from men with cancer were exposed to six refreeze-thawing cycles. Although recovery of motile spermatozoa was significantly impaired after each thawing, there were no significant differences in the recovery of motile sperm between thaws in cancer and non-cancer groups. Conclusions: Human spermatozoa resist repeated cryopreservation using the fast liquid nitrogen vapor method. Normozoospermic specimens withstand refreezing for an average two cycles longer than oligozoospermic ones. Specimens from cancer patients seem to resist repeated cryoinjury similarly to non-cancer counterparts. Resistance to repeated cryoinjury was related to the initial semen quality.

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Section: Normozoospermicmentioning

confidence: 96%

Resistance of human spermatozoa to cryoinjury in repeated cycles of thaw-refreezing

Verza¹,

Feijó²,

Esteves³

2009

Int. braz j urol.

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“…The goals of this novel strategy were to alleviate stress-induced anejaculation on the day of oocyte retrieval [2,3] and to maximize quality and numbers of motile sperm available by eliminating potential iatrogenic cryopreservation-induced damage, further loss of alreadydiminished numbers of motile sperm following cryopreservation, and eliminating unpredictability of between-and withinpatient post-thaw sperm viability [4][5][6][7]. And, while we stipulate that in most, if not all cases, there would likely have been sufficient sperm in a single ejaculate from these men for ICSI, Capsule Pooling consecutive ejaculates produced within 24 h significantly increased the numbers of available motile sperm for IVF in asthenozoospermic and oligoasthenozoospermic men by 8-fold over a single ejaculate alone.…”

Section: Introductionmentioning

confidence: 99%

Increased count, motility, and total motile sperm cells collected across three consecutive ejaculations within 24 h of oocyte retrieval: implications for management of men presenting with low numbers of motile sperm for assisted reproduction

Said

Reed

2015

J Assist Reprod Genet

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Purpose The purpose of this study was to quantitate changes in seminal volume, sperm count, motility, qualitative forward progression, and total motile sperm cells per ejaculate, across three consecutive ejaculates collected from individuals within 24 h preceding an IVF cycle. Methods Men presenting with oligoasthenozoospermia or asthenozoospemia attempted three ejaculates within 24 h preceding IVF. Ejaculate 1 was produced the afternoon prior to oocyte retrieval, and ejaculates 2 and 3 were produced the morning of oocyte retrieval with 2-3 h between collections. Ejaculates 1 and 2 were extended 1:1 v/v with room temperature rTYBS. Test tubes were placed into a beaker of room temperature water, then placed at 4°C for gradual cooling. Ejaculate 3 was not extended, but pooled with ejaculates 1 and 2 and processed for intracytoplasmic sperm injection (ICSI). Results Out of 109 oocyte retrievals, 28 men were asked to attempt multiple consecutive ejaculations. Among this population, 25/28 (89.3 %) were successful, and 3/28 men (10.7 %) could only produce two ejaculates. Mean volumes for ejaculates 1, 2, and 3 were significantly different from each other (p<0.01); the volume decreased for each ejaculate. Mean sperm counts, motility, qualitative forward progression, and total motile cells per ejaculate for the ejaculates1, 2, and 3 demonstrated the following: ejaculates 2 and 3 were not significantly different, but counts, motility, and total motile sperm were improved over ejaculate 1 (p<0.01). Conclusions Pooling three consecutive ejaculates within 24 h increased the numbers of available motile sperm in this population by 8-fold compared to the first ejaculate alone, facilitating avoidance of sperm cryopreservation and additional centrifugation steps that could affect sperm viability and/or function.

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“…However, the results of the effect of pentoxifylline change according to the moment of addition, before (ESTEVES et al, 1998;SPAINE;CEDENHO, 2007) Therefore, Calogero et al (1998) examined the intracellular cAMP content. In some individuals pentoxifylline induced changes in sperm kinetic characteristics and hyperactivation were not always accompanied by an equivalent change in the intracellular cAMP content.…”

Section: Effect Of Equine Thawed Sperm Dilution Using Skim Milk Extenmentioning

confidence: 99%

“…Pre-freeze treatment of poor quality human sperm with pentoxifylline improved the ability of thawed spermatozoa to undergo the acrosome reaction in response to calcium ionophore (ESTEVES; SPAINE; CEDENHO, 2007). …”

Section: Effect Of Pentoxifyline Addition To Skim Milk Extender To Frmentioning

confidence: 99%

“…Besides pentoxifylline is a phosphodiesterase inhibitor and increases sperm kinetic characteristics and the number of spermatozoa exhibiting hyperactivated motility (CALOGERO et al, 1998;SPAINE;CEDENHO, 2007) appears to participate from two concurrent methods of action: it acts as a rheological agent, improving blood flow to compromised tissues, and it acts as an immunomodulatory agent (BAUMGARTNER, 2007).…”

Section: The Effect Of Semen Extender Supplemented With Pentoxifyllinmentioning

confidence: 99%

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Efeitos da pentoxifilina sobre a qualidade espermática e hemodinâmica uterina em equinos

Tsunoda¹

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AGRADECIMENTOSÀ Deus, por fazer com que tudo sempre ocorra de forma melhor do que eu tinha planejado, por ter permitido que esse experimento ocorresse, o qual me trouxe tantas aprendizagens tanto para a vida profissional como para a pessoal;À minha família, obrigada por estarem sempre ao meu lado, me incentivarem e apoiarem na concretização de um sonho, que souberam compreender meus períodos de ausência;Ao Cássio, obrigada pelo companheirismo, incentivo diário, por torcer por mim, por me acalmar e me ajudar a enfrentar meus medos e minha ansiedade;À professora Eneiva Carla, obrigada pela oportunidade, pela amizade, por ter confiado em mim. Professora, você é um exemplo de ser humano para mim, honesta e humilde, com quem tive muito prazer em trabalhar;À Bruna Marcele Oliveira, obrigada pela amizade, pelo apoio desde que entrei para a equipe, obrigada por ter aceitado a aventura, pelo companheirismo em momentos difíceis, e pelas risadas que ficaram de tudo que passamos juntas;À minha ex-equipe, Andressa, Diego João, Rodrigo obrigada pelos ensinamentos, pela amizade, pelas risadas, pelas viagens de projetos, foi um prazer conhecê-los e conviver com cada um de vocês;Ao meu amigo querido Marcilio, quem não mediu esforços para me ajudar nesta Dissertação, obrigada pelos ensinamentos, pela paciência, pelos conselhos, por ter estado ao meu lado desde que cheguei à USP; À Mariana Rodrigues, obrigada pela amizade, pelo companheirismo, por me ouvir tantas vezes que precisei e por me ajudar a enxergar uma saída em dias tensos; À Carolina Rocha, obrigada por tudo que já fez por mim, pela amizade que construímos no decorrer deste mestrado, você faz muita falta no meu dia-dia. Pentoxifylline addition did not exert effect on progressive motility, however, pentoxifylline increased path velocity, progressive velocity and curvilinear velocity when compared to controls. There were no significant differences on the percentage of cells presenting intact plasma membrane, intact acrosome and high mitochondrial membrane potential (IPIAH). No statistical difference was found on uterine blood flow of mares after treatments. However, endometrial cytology showed a major inflammatory response on groups containingPentoxifylline when compared to controls. In conclusion, pentoxifylline exerted a positive effect on velocity parameters, but do not influence cellular membranes and chromatin.Furthermore, when mares were inseminated with semen containing pentoxifylline, an intense inflammatory response was observed.

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Effects of pentoxifylline treatment before freezing on motility, viability and acrosome status of poor quality human spermatozoa cryopreserved by the liquid nitrogen vapor method

Cited by 45 publications

References 26 publications

Resistance of human spermatozoa to cryoinjury in repeated cycles of thaw-refreezing

Resistance of human spermatozoa to cryoinjury in repeated cycles of thaw-refreezing

Increased count, motility, and total motile sperm cells collected across three consecutive ejaculations within 24 h of oocyte retrieval: implications for management of men presenting with low numbers of motile sperm for assisted reproduction

Efeitos da pentoxifilina sobre a qualidade espermática e hemodinâmica uterina em equinos

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