Effects of pentoxifylline on immediate and late-phase cutaneous reactions in response to anti–immunoglobulin E antibodies in clinically normal dogs

Abstract

In clinically normal dogs, pentoxifylline effectively impaired late-phase reactions but not immediate reactions at sites of intradermal injection of IgE-specific antibodies by inhibiting mast cell degranulation and recruitment of cutaneous inflammatory cells, especially eosinophils.

Characterization of the Pro-Inflammatory and Pruritogenic Transcriptome in Skin Lesions of the Experimental Canine Atopic Acute IgE-Mediated Late Phase Reactions Model and Correlation to Acute Skin Lesions of Human Atopic Dermatitis

“…Intradermal injection of anti-canine-IgE in healthy dogs results in immediate and late-phase reactions (LPR); LPRs follow 3 to 48 h after allergen challenge and are accompanied by inflammatory cell infiltration that histologically resembles changes seen in naturally occurring canine atopic dermatitis [ 4 , 5 ]. Therefore, cutaneous IgE-mediated LPRs have been used as a screening atopic dermatitis model for studying the anti-inflammatory effect of anti-allergic drugs before entering clinical trials [ 6 , 7 , 8 , 9 ]. Interestingly, a pilot quantitative reverse-transcription PCR analysis of canine IgE-mediated LPRs with only a few genes investigated revealed increased expression of proallergic cytokine interleukin-13 ( IL-13 ) and CC chemokine ligand 5 ( CCL5 ) and CCL17 [ 5 ].…”

Insights into immunoglobulin E‐mediated late‐phase reactions in dogs

“…This was confirmed later on by Hillier et al . ; The authors emphasize the similarity of the observed phenomena to the morphological changes seen in the infiltrates of atopic dermatitis in dogs and conclude that the cutaneous late‐phase reaction induced in these animals could be an excellent pharmacological model in which to investigate anti‐allergic or anti‐atopic pharmacological agents …”