1986
DOI: 10.1111/j.1365-2133.1986.tb02795.x
|View full text |Cite
|
Sign up to set email alerts
|

Effects of pemphigus antibody on the organization of microtubules and keratin-intermediate filaments in cultured human keratinocytes

Abstract: It has been suggested that pemphigus antibodies (PA) react with the surface molecules on keratinocytes, and induce the production and release of proteases resulting in acantholysis. If this is the case, the immunoreactions on the cell surface may send signals to the interior of the cell across the membrane. The present study was carried out to determine whether or not cytoskeletons [microtubules (MT) and keratin-intermediate filaments (KIF)] respond to PA-immunoreactions in cultured human keratinocytes, by ind… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

1
19
1
1

Year Published

1989
1989
2008
2008

Publication Types

Select...
8

Relationship

0
8

Authors

Journals

citations
Cited by 33 publications
(22 citation statements)
references
References 19 publications
1
19
1
1
Order By: Relevance
“…The first proposes that anti-Dsg3 antibody-dependent steric hindrance interferes with intercellular adhesive function(s) of Dsg3, leading directly to desmosomal dissociation (12)(13)(14). However, we have observed no inhibition of Ca 2ϩ -induced desmosome formation by PV-IgG binding to surface PV-antigens (15), suggesting that PV-IgG does not directly inhibit desmosome formation, even though the antibodies may cause steric hindrance between homophilic Dsg3 interactions.…”
mentioning
confidence: 63%
See 1 more Smart Citation
“…The first proposes that anti-Dsg3 antibody-dependent steric hindrance interferes with intercellular adhesive function(s) of Dsg3, leading directly to desmosomal dissociation (12)(13)(14). However, we have observed no inhibition of Ca 2ϩ -induced desmosome formation by PV-IgG binding to surface PV-antigens (15), suggesting that PV-IgG does not directly inhibit desmosome formation, even though the antibodies may cause steric hindrance between homophilic Dsg3 interactions.…”
mentioning
confidence: 63%
“…Cell Culture-DJM-1 cells, a cell line isolated from human skin squamous cell carcinoma (15) were seeded either in 35-mm dishes or on glass coverslips. Cells were cultured in Eagle's minimum essential medium (MEM) (Nissui Phama, Tokyo, Japan) containing 10% fetal calf serum, 0.4 g/ml hydrocortisone (Sigma), 20 ng/ml epidermal growth factor (TOYOBO, Osaka, Japan), 84 ng/ml cholera toxin (List Biological Laboratories, Campbell, CA), and 1.8 mM Ca 2ϩ .…”
Section: Methodsmentioning
confidence: 99%
“…The primary events after binding of pemphigus antibodies to keratinocytes are changes in the cytoskeleton of keratinocytes with retraction of tonofilaments [17][18][19] and breaking of the bond between tonofilaments and desmosomes culminating in shrinkage of the basal cells. 8,[20][21][22] The detachment of tonofilaments occurs before the desmosomes show any visible alteration 8,23 or loss in adhesion.…”
Section: Nondesmosomal Mechanisms Appear To Be Involved In Pv Acanthomentioning
confidence: 99%
“…DJM-1 cells, a human squamous cell carcinoma cell line (Kitajima et al, 1986(Kitajima et al, , 1987, were cultured in s-MEM (Life Technologies, Inc.) supplemented with 10% (v/v) Ca 2+ -chelated fetal bovine serum (IS-Japan, Tokyo, Japan), 84 ng/ml choleratoxin (Life Technologies, Inc.) and 0.4 mg/ ml hydrocortisone (Sigma) and CaCl2 (the final Ca 2+ concentration of the medium was adjusted to 0.09 mM). Cells were cultured on 24´24 mm glass coverslips (Matsunami Glass Co. Tokyo, Japan) for immunofluorescent microscopy in 35 mm cell culture dishes (Becton-Dickinson and Co., Franklin Lakes, NJ, USA) and 100 mm cell culture dishes (Corning Costar Corp., Cambridge, MA, USA) for biochemical study in 37°C CO2-incubator.…”
Section: Cellsmentioning
confidence: 99%