Effects of paternal diet and antioxidant addition to the semen extender on bovine semen characteristics and on the phenotype of the resulting embryo

Bastos, Natália Marins; Rossi, Guilherme Fazan; Leão, Beatriz Caetano da Silva; Negrão, Fernanda; Ferreira, Christina R.; Vrisman, Dayane Priscila; Rodrigues, Naiara Nantes; Zorzetto, Mariana Furtado; Freitas‐Dell’Aqua, Camila de Paula; Vantini, Roberta; Monteiro, Fábio Morato; Mercadante, Maria Eugênia Zerlotti; Eberlin, Marcos N.; Lima, Vera Fernanda Martins Hossepian de; Mingoti, Gisele Zoccal

doi:10.1016/j.theriogenology.2021.08.031

Cited by 4 publications

(4 citation statements)

References 58 publications

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“…In agreement with this, lack of omega-3 in the diet of fatty acid desaturase-2 knockout mice affected the spermatid maturation and acrosome formation [68]. On the other hand, feeding young bulls with PUFA increased the proportion of frozen-thawed spermatozoa with damaged acrosomal membrane [19]. Therefore, the effect of omega-3 supplementation on acrosome membranes remains an open question.…”

Section: Plos Onementioning

confidence: 65%

“…Supporting this, a previous study on dairy cows indicated the beneficial impact of a flaxseed oil-enriched diet on the developing blastocyst, expressed by a high number of blastomeres in the developing embryos [93], and reduced incidence of pregnancy loss [94]. In contrast, Bastos et al [19] did not find any effect on cleavage or the blastocyst-formation rate following PUFA supplementation to young bulls. The difference between findings might be related to differences in bull age or genotype, the source of supplement, or the duration of the feeding trial.…”

Section: Plos Onementioning

confidence: 80%

“…Feeding a flaxseed oil-enriched diet to Holstein bulls increased the spermatozoa progressive motility and velocity in fresh semen [8]. In contrast, in a recent study, PUFA enrichment of diets fed to young Nellore bulls for 10 months had a negative impact on their spermatozoa quality characteristics, including motility, membrane integrity, and lipid proportions [19].…”

Section: Introductionmentioning

confidence: 79%

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Carryover effects of feeding bulls with an omega-3-enriched-diet—From spermatozoa to developed embryos

et al. 2022

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The impact of omega-3 nutritional manipulation on semen cryosurvival and quality post thawing is controversial. Our aim was to examine how feeding bulls with omega-3 supplementation from different sources affects the spermatozoa quality parameters. Fifteen Israeli Holstein bulls were fed for 13 weeks with a standard ration top-dressed with encapsulated-fat supplementation: fish or flaxseed oil or saturated fatty acids (control). Ejaculates were collected before, during, and after the feeding trial. Frozen–thawed samples were evaluated by a flow cytometer for spermatozoa viability, mitochondrial membrane potential, the level of reactive oxygen species (ROS), acrosome membrane integrity, DNA fragmentation, phosphatidylserine translocation, and membrane fluidity. Both fish and flaxseed oil treatment resulted in lower ROS levels vs. control groups, during and after the feeding trial. Fewer spermatozoa with damaged acrosomes were observed in the fish oil group after the feeding trial. The spermatozoa membrane fluidity was altered in both the fish and flaxseed oil groups throughout the feeding trial, but only in the flaxseed oil group after the feeding trial. The proportion of spermatozoa with fragmented DNA was lower in the flaxseed oil group after the feeding trial. The spermatozoa fertilization competence did not differ between groups however, blastocyst formation rate was higher in the fish and flaxseed oil groups relative to the control. This was associated with differential gene expression in the blastocysts. Overall, the omega-3-enriched food improved the spermatozoa characteristics; this was further expressed in the developing blastocysts, suggesting a carryover effect from the spermatozoa to the embryos.

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Section: Plos Onementioning

confidence: 65%

Section: Plos Onementioning

confidence: 80%

Section: Introductionmentioning

confidence: 79%

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Carryover effects of feeding bulls with an omega-3-enriched-diet—From spermatozoa to developed embryos

et al. 2022

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“…Indeed, the damage caused by ROS could be irreversible, regardless of the amount of antioxidants secreted, making it a tedious task to accurately assess sperm quality and fertility capacities. For this reason several studies have shown the role of supplementation of different antioxidants, such as melatonin, resveratrol, and catalase, on the freezing and/or thawing media [ 52 , 53 , 54 ].…”

Section: Discussionmentioning

confidence: 99%

Relationships between Biomarkers of Oxidative Stress in Seminal Plasma and Sperm Motility in Bulls before and after Cryopreservation

Vigolo

Giaretta

Dalt

et al. 2022

Animals

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This study aimed at evaluating the relationship between biomarkers of oxidative stress (OS) in seminal plasma and sperm motility in bulls before and after cryopreservation. Three ejaculates per bull were collected from 20 young bulls. Each ejaculate was analyzed for motility before and after cryopreservation (by CASA), and the SP concentration of Advanced Oxidation Protein Products (AOPP), thiols, and carbonyl groups (CT) were examined. Then, based on their motility, the ejaculates were grouped into: high motility fresh (HMF), low motility fresh (LMF), high motility thawed (HMT), and low motility thawed (LMT) groups. Higher AOPP and thiol concentrations on SP were related (p < 0.05) to the higher LIN and BCF and lower ALH of fresh semen. In addition, AOPP and thiols were significantly higher in HMF than LMF. As a confirmation of this, the Receiver Operating Characteristic (ROC) curve analysis showed that AOPP and thiol concentrations in SP were able to discriminate between HMF and LMF ejaculates (Area Under the Curve of 71.67% and 72.04%, respectively). These observations give an alternative perspective on the relationship between sperm motility and the OS parameters of SP, which need further investigations.

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Lipids in the reproduction of sires

Beltrão,

de Castro Ferraz Junior

2023

Trop Anim Health Prod

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Effects of paternal diet and antioxidant addition to the semen extender on bovine semen characteristics and on the phenotype of the resulting embryo

Cited by 4 publications

References 58 publications

Carryover effects of feeding bulls with an omega-3-enriched-diet—From spermatozoa to developed embryos

Carryover effects of feeding bulls with an omega-3-enriched-diet—From spermatozoa to developed embryos

Relationships between Biomarkers of Oxidative Stress in Seminal Plasma and Sperm Motility in Bulls before and after Cryopreservation

Lipids in the reproduction of sires

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