Effects of paternal cigarette smoking on testicular function, sperm fertilizing capacity, embryonic development, and blastocyst capacity for implantation in rats

Kapawa, A.; Giannakis, Dimitrios; Tsoukanelis, K.; Kanakas, N.; Baltogiannis, D.; Agapitos, E.; Loutradis, Dimitrios; Miyagawa, Ikuo; Sofikitis, Nikolaos

doi:10.1111/j.1439-0272.2004.00605.x

Cited by 67 publications

(73 citation statements)

References 53 publications

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“…Our results with MS smoke confirm previous findings in rats that showed significant decreases in fertilization and embryonic cleavage (Kapawa et al, 2004). However, the effects observed in the current study with lower exposure levels (cotinine level of 95.4 ± 23.5 ng/mL vs.…”

Section: Discussionsupporting

confidence: 42%

“…Delayed early embryonic development has been associated with the generation of blastocysts with lowered capacity for implantation and reduced numbers of live offspring (Kapawa et al, 2004). Our results suggest that paternal exposure to MS smoke alters sperm genetic or epigenetic factors that affect the developmental capacity of the fertilized embryo.…”

Section: Discussionmentioning

confidence: 68%

“…Rodent studies have corroborated these findings. Exposing male mice to mainstream (MS) tobacco smoke, the main smoke inhaled by active smokers, induced genetic mutations in sperm (Yauk et al, 2007), reduced sperm fertilizing capacity (Kapawa et al, 2004), and decreased embryonic implantation rates (Kapawa et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

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Differential sensitivity of male germ cells to mainstream and sidestream tobacco smoke in the mouse

Polyzos

Schmid

Piña-Guzmán

et al. 2009

Toxicology and Applied Pharmacology

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Cigarette smoking in men has been associated with increased chromosomal abnormalities in sperm and with increased risks for spontaneous abortions, birth defects and neonatal death.Little is known, however, about the reproductive consequences of paternal exposure to secondhand smoke. We used a mouse model to investigate the effects of paternal exposure to sidestream (SS) smoke, the main constituent of second-hand smoke, on the genetic integrity and function of sperm, and to determine whether male germ cells were equally sensitive to mainstream (MS) and SS smoke. A series of sperm DNA quality and reproductive endpoints were investigated after exposing male mice for two weeks to MS or SS smoke. Our results indicated that: (i) only SS smoke significantly affected sperm motility; (ii) only MS smoke induced DNA strand breaks in sperm; (iii) both MS and SS smoke increased sperm chromatin structure abnormalities; and (iv) MS smoke affected both fertilization and the rate of early embryonic development, while SS smoke affected fertilization only. These results show that MS and SS smoke have differential effects on the genetic integrity and function of sperm and provide further evidence that male exposure to second-hand smoke, as well as direct cigarette smoke, may diminish a couple's chance for a successful pregnancy and the birth of a healthy baby.

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Section: Discussionsupporting

confidence: 42%

Section: Discussionmentioning

confidence: 68%

See 1 more Smart Citation

Differential sensitivity of male germ cells to mainstream and sidestream tobacco smoke in the mouse

Polyzos

Schmid

Piña-Guzmán

et al. 2009

Toxicology and Applied Pharmacology

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show abstract

“…It has been demonstrated that oocytes injected with spermatozoa with high motility have a significantly higher chance of reaching the blastocyst stage [30,43]. It has also been reported that paternal cigarette smoke exposure affects the embryonic implantation ability [21]. However, the results of the above-mentioned studies should be interpreted with caution because some of these results were based on the continued culture of embryos that were considered unsuitable for transfer or cryopreservation on day three of development.…”

Section: Discussionmentioning

confidence: 99%

Patient selection criteria for blastocyst transfers in extended embryo culture programs

Braga

Setti

Figueira

et al. 2012

J Assist Reprod Genet

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Purpose To identify the correlation between different cycles, patient factors and blastocyst characteristics. Methods The study included 420 patients undergoing ICSI cycles and 2781 graded blastocysts, which took into account the blastocyst quality. The correlations between the blastocyst parameters and the patient and cycle characteristics were assessed. Results The blastocyst development was negatively correlated with the maternal age, BMI and dose of FSH. The ICM was negatively correlated with the FSH dose, whereas the TE quality was influenced by the FSH dose, the maternal age and the number of retrieved oocytes. The embryo morphology on days two and three may predict the blastocyst developmental competence. Conclusions Older patients and patients with high BMI should not be included in extended embryo culture programmes. The extended culture may not favour embryos with poor morphology on days two and three of development. Additionally, a lower ovarian stimulation and decreased oocyte yields may lead to the development of high-quality blastocysts.

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“…Kapawa et al (22) detected that exposed to tobacco smoke for 10 weeks decreased in the percentage of motile sperm.…”

Section: Discussionmentioning

confidence: 99%

Antioxidant effects of ellagic acid on testicular tissue of rats exposed to tobacco smoke

Kaya¹,

Ozan²,

Dabak³

et al. 2017

J Turgut Ozal Med Cent

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Aim: Tobacco smoke negatively affects the male reproductive system. Ellagic acid (EA) has protective effects against oxidative damage. The aim of the study was to examine the protective effects of EA on testis of rats exposed to tobacco smoke. Material and Methods: Twenty-four male Spraque-Dawley rats were divided into 4 groups (n=6): Control, tobacco smoke (TS), tobacco smoke+corn oil (TS+C) and tobacco smoke+ EA (TS+EA). TS, TS+C and TS+EA groups were exposed to tobacco smoke 1 hour twice a day and EA was applied 12 mg/kg every other day. Testis tissues were removed. eNOS immunohistochemical stain and TUNEL methods were applied. Biochemical analyzes and sperm analyzes were performed. Results: Degeneration in germinative epithelium, cell debris in the seminiferous tubule lumen, seperation in basement membrane, atrophic tubules, vascular congestion and edema in interstitial area were observed in TS and TS+C groups. Increased apoptotic cells and eNOS immunreactivity were observed in TS and TS+C groups. EA administration caused a decrease in histological alterations, eNOS immunreactivity and apoptotic cells. Increased MDA levels, decreased CAT and GSH-Px activities were observed in TS and TS+C groups. MDA levels decreased, CAT and GSH-Px activities increased in TS+EA group. A significant increase in the amount of abnormal sperm was detected in TS and TS+C groups. The reduction in the amount of abnormal sperm was detected in TS+EA group. Conclusions: Exposure to TS led to marked alterations on testes tissue and treatment with EA might prevent these toxic effects.

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Effects of paternal cigarette smoking on testicular function, sperm fertilizing capacity, embryonic development, and blastocyst capacity for implantation in rats

Cited by 67 publications

References 53 publications

Differential sensitivity of male germ cells to mainstream and sidestream tobacco smoke in the mouse

Differential sensitivity of male germ cells to mainstream and sidestream tobacco smoke in the mouse

Patient selection criteria for blastocyst transfers in extended embryo culture programs

Antioxidant effects of ellagic acid on testicular tissue of rats exposed to tobacco smoke

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