Effects of particulate matter exposure on semen quality: A retrospective cohort study

Guan, Quanquan; Chen, Shiyao; Wang, Bingqian; Dou, Xiao-Yu; Lu, Yue; Liang, Jian; Ni, Rong; Yang, Chao; Wang, Hengbin; Baktash, Mohammad Basir; Wu, Wei; Wang, Xinru; Fu, Guangbo; Xia, Yankai

doi:10.1016/j.ecoenv.2020.110319

Cited by 34 publications

(17 citation statements)

References 37 publications

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“…Numerous epidemiology studies have shown PM 2.5 could affect male reproduction and lead to a decrease in sperm quality (7)(8)(9). Our previous study also indicated PM 10-2.5 exposure was inversely associated with sperm concentration in a longitudinal study of 796 Chinese college students (10).…”

Section: Introductionmentioning

confidence: 54%

Analysis by Metabolomics and Transcriptomics for the Energy Metabolism Disorder and the Aryl Hydrocarbon Receptor Activation in Male Reproduction of Mice and GC-2spd Cells Exposed to PM2.5

et al. 2022

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Fine particulate matter (PM2.5)-induced male reproductive toxicity arouses global public health concerns. However, the mechanisms of toxicity remain unclear. This study aimed to further investigate toxicity pathways by exposure to PM2.5in vitro and in vivo through the application of metabolomics and transcriptomics. In vitro, spermatocyte-derived GC-2spd cells were treated with 0, 25, 50, 100 μg/mL PM2.5 for 48 h. In vivo, the real-world exposure of PM2.5 for mouse was established. Forty-five male C57BL/6 mice were exposed to filtered air, unfiltered air, and concentrated ambient PM2.5 in Tangshan of China for 8 weeks, respectively. The results in vitro and in vivo showed that PM2.5 exposure inhibited GC-2spd cell proliferation and reduced sperm motility. Mitochondrial damage was observed after PM2.5 treatment. Increased Humanin and MOTS-c levels and decreased mitochondrial respiratory indicated that mitochondrial function was disturbed. Furthermore, nontargeted metabolomics analysis revealed that PM2.5 exposure could disturb the citrate cycle (TCA cycle) and reduce amino acids and nucleotide synthesis. Mechanically, the aryl hydrocarbon receptor (AhR) pathway was activated after exposure to PM2.5, with a significant increase in CYP1A1 expression. Further studies showed that PM2.5 exposure significantly increased both intracellular and mitochondrial reactive oxygen species (ROS) and activated NRF2 antioxidative pathway. With the RNA-sequencing technique, the differentially expressed genes induced by PM2.5 exposure were mainly enriched in the metabolism of xenobiotics by the cytochrome P450 pathway, of which Cyp1a1 was the most significantly changed gene. Our findings demonstrated that PM2.5 exposure could induce spermatocyte damage and energy metabolism disorder. The activation of the aryl hydrocarbon receptor might be involved in the mechanism of male reproductive toxicity.

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Section: Introductionmentioning

confidence: 54%

Analysis by Metabolomics and Transcriptomics for the Energy Metabolism Disorder and the Aryl Hydrocarbon Receptor Activation in Male Reproduction of Mice and GC-2spd Cells Exposed to PM2.5

et al. 2022

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“…They are actively released by many types of cells and are widely distributed in saliva, plasma, milk, amniotic fluid, and other body fluids [ 9 , 60 ]. EVs participate in intercellular information transmission and regulate the recipient cells by binding to specific receptors on the cell surface, directly fusing with the cell membrane of target cells, or been taken up by recipient cells through endocytosis and releasing their contents to the recipient cells [ 61 – 63 ]. The functions of EVs from different cell sources are closely associated with their respective microenvironments.…”

Section: Discussionmentioning

confidence: 99%

Mesenchymal stem cell-derived extracellular vesicles prevent the development of osteoarthritis via the circHIPK3/miR-124-3p/MYH9 axis

Liu

et al. 2021

J Nanobiotechnol

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Background Extracellular vesicles (EVs) secreted by mesenchymal stem cells (MSCs) may play a vital role in a variety of biological processes, including cartilage regeneration. However, few studies reported their potential in the development of osteoarthritis (OA) previously. In this study, we explored the biological roles and underlying mechanism of MSCs-EVs in OA. Results Co-culture experiments revealed that MSCs-EVs could promote the expression of collagen type II alpha 1 chain (COL2A1), SRY-box transcription factor 9 (SOX9) and Aggrecan while negatively regulate the expression of chondrocyte hypertrophy markers matrix metallopeptidase 13 (MMP-13) and RUNX family transcription factor 2 (Runx2) in mouse chondrocytes in the OA model. Besides, the results of cell experiments indicated that MSCs-EVs could notably weaken the suppression of chondrocyte proliferation, migration and the promotion of chondrocyte apoptosis via interleukin1β (IL-1β) induction. In addition, MSCs-circHIPK3-EVs (EVs derived from MSCs overexpressing circHIPK3) considerably improved IL-1β-induced chondrocyte injury. Mechanistically, we elucidated that circHIPK3 could directly bind to miR-124-3p and subsequently elevate the expression of the target gene MYH9. Conclusion The findings in our study demonstrated that EVs-circHIPK3 participated in MSCs-EVs-mediated chondrocyte proliferation and migration induction and in chondrocyte apoptosis inhibition via the miR-124-3p/MYH9 axis. This offers a promising novel cell-free therapy for treating OA. Graphic abstract

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“…Previous studies showed inconsistent results with regard to the association between particular matter exposure and semen quality. While a couple of studies showed a negative association between air pollution and progressive rate ( Guan et al, 2020 ; Huang et al, 2020 ), studies such as the one conducted by Lao et al in Taiwan on 6475 male participants between 2001 and 2014 showed exposure to ambient PM 2.5 air pollution was associated with higher level of sperm concentration (2018). Similarly, Zhou et al found PM 10 exposure has shown positive associations with progressive rate (2018).…”

Section: Discussionmentioning

confidence: 99%

Semen quality and windows of susceptibility: A case study during COVID-19 outbreak in China

Yang

Deng

Sun

et al. 2021

Environmental Research

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Background To evaluate the impact of air pollution exposure on semen quality parameters during COVID-19 outbreak in China, and to identify potential windows of susceptibility for semen quality. Methods A retrospective observational study was carried out on 1991 semen samples collected between November 23, 2019 and July 23, 2020 (a period covering COVID-19 lock-down in China) from 781 sperm donor candidates at University-affiliated Sichuan Provincial Human Sperm Bank. Multivariate mixed-effects regression models were constructed to investigate the relationship between pollution exposure, windows of susceptibility, and semen quality, while controlling for biographic and meteorologic confounders. Result(s) The results indicated multiple windows of susceptibility for semen quality, especially sperm motility, due to ambient pollution exposure. Exposure to particulate matters (PM 2.5 and PM 10 ), O 3 and NO 2 during late stages of spermatogenesis appeared to have weak but positive association with semen quality. Exposure to CO late in sperm development appeared to have inverse relationship with sperm movement parameters. Exposure to SO 2 appeared to influence semen quality throughout spermatogenesis. Conclusion(s) Potential windows of susceptibility for semen quality varied depending on air pollutants. Sperm motility was sensitive to pollution exposure. Findings from current study further elucidate the importance of sensitive periods during spermatogenesis and provide new evidence for the determinants of male fertility.

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Effects of particulate matter exposure on semen quality: A retrospective cohort study

Cited by 34 publications

References 37 publications

Analysis by Metabolomics and Transcriptomics for the Energy Metabolism Disorder and the Aryl Hydrocarbon Receptor Activation in Male Reproduction of Mice and GC-2spd Cells Exposed to PM2.5

Analysis by Metabolomics and Transcriptomics for the Energy Metabolism Disorder and the Aryl Hydrocarbon Receptor Activation in Male Reproduction of Mice and GC-2spd Cells Exposed to PM2.5

Mesenchymal stem cell-derived extracellular vesicles prevent the development of osteoarthritis via the circHIPK3/miR-124-3p/MYH9 axis

Semen quality and windows of susceptibility: A case study during COVID-19 outbreak in China

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