Effects of paroxetine on spatial memory function and protein kinase C expression in a rat model of depression

Han, Jianbo; Wang, L U; Bian, Hongyan; Zhou, Xiaoyan; Ruan, Cailian

doi:10.3892/etm.2015.2663

Cited by 16 publications

(12 citation statements)

References 11 publications

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“…S2). We found that thalidomide, paroxetine, and methoxamine increased the percentage of counterclockwise (CCW) cells, suggesting a potential role of PKC in cardiac cell chirality (31–34). Moreover, our work from the 2D micropatterning system with clockwise cells such as human umbilical vein endothelial cell (HUVEC) lines (27) demonstrated that the activation of PKC signaling reverses chirality of endothelial cells from clockwise (CW) to CCW (35).…”

Section: Resultsmentioning

confidence: 88%

Intrinsic cellular chirality regulates left–right symmetry breaking during cardiac looping

Ray

Chin

Worley

et al. 2018

Proc. Natl. Acad. Sci. U.S.A.

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The vertebrate body plan is overall symmetrical but left–right (LR) asymmetric in the shape and positioning of internal organs. Although several theories have been proposed, the biophysical mechanisms underlying LR asymmetry are still unclear, especially the role of cell chirality, the LR asymmetry at the cellular level, on organ asymmetry. Here with developing chicken embryos, we examine whether intrinsic cell chirality or handedness regulates cardiac C looping. Using a recently established biomaterial-based 3D culture platform, we demonstrate that chick cardiac cells before and during C looping are intrinsically chiral and exhibit dominant clockwise rotation in vitro. We further show that cells in the developing myocardium are chiral as evident by a rightward bias of cell alignment and a rightward polarization of the Golgi complex, correlating with the direction of cardiac tube rotation. In addition, there is an LR polarized distribution of N-cadherin and myosin II in the myocardium before the onset of cardiac looping. More interestingly, the reversal of cell chirality via activation of the protein kinase C signaling pathway reverses the directionality of cardiac looping, accompanied by a reversal in cellular biases on the cardiac tube. Our results suggest that myocardial cell chirality regulates cellular LR symmetry breaking in the heart tube and the resultant directionality of cardiac looping. Our study provides evidence of an intrinsic cellular chiral bias leading to LR symmetry breaking during directional tissue rotation in vertebrate development.

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Section: Resultsmentioning

confidence: 88%

Intrinsic cellular chirality regulates left–right symmetry breaking during cardiac looping

Ray

Chin

Worley

et al. 2018

Proc. Natl. Acad. Sci. U.S.A.

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“…While a certain extent of standardization of the procedure may be desirable, there are no standard factors that distinguish the laboratories in which the procedure operates reliably from those where it does not (39). Anhedonia is one of the primary symptoms of depression, which can be inferred from a reduction in the intake of sweet liquid food (1,40). As expected, the SPP of rats decreased significantly following the CUMS procedure in the present study, which was consistent with results of a previous study (7).…”

Section: Discussionmentioning

confidence: 99%

“…Depression is a prevalent and recurrent psychological illness that results in a significant burden for individuals and the society (1). Previous studies indicated that electroconvulsive therapy (ECT) is an efficient and rapid treatment for major depression, especially for patients with drug-resistant and refractory depression (2,3).…”

Section: Introductionmentioning

confidence: 99%

Effect of different charges of modified electroconvulsive seizure on the cognitive behavior in stressed rats: Effects of GluR1 phosphorylation and CaMKIIα activity

2018

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Electroconvulsive therapy (ECT) is an efficient therapy for major depression and modern ECT requires anesthesia to enhance safety. However, the commonly used anesthetic, propofol, may weaken the treatment efficacy. A recent study confirmed that ketamine rapidly reduced the symptoms of depression in affected patients. A previous study found that electroconvulsive seizure (ECS), the animal model for ECT, under anesthesia of low-dose ketamine combined with propofol could enhance the antidepressant efficacy and improve the cognitive performance. The present study aimed to investigate the responses to different charges (0, 60, 120, 180 or 240 mC) of ECS under compound anesthetics, ketamine combined with propofol, in stressed rats and the underlying mechanisms to aid in optimization of treatment regimens. The results indicated that ECS exhibited an improved antidepressant effects at 120 mC compared with 60 mC, however, no significant differences in antidepressant effects were identified among the 120, 180 and 240 mC groups. Furthermore, rats subjected to ECS at 120 mC exhibited the best cognitive performance. The phosphorylation levels of calcium/calmodulin-dependent protein kinase IIα (CaMKIIα) at Thr286, glutamate receptor 1 (GluR1) at Ser831 and cAMP-response element-binding protein (CREB) at the Ser133 were higher in the 120-mC group compared with all other groups. These results indicated that the ECS at medium intensity (120 mC) with administration of compound anesthetics may exert an improved therapeutic effect on depression compared with other intensities (0, 60, 180 and 240 mC). The results also suggested that the improvement in cognitive function in stressed rats may be attributed to the phosphorylation of CaMKIIα (Thr286), GluR1 (Ser831) and CREB (Ser133).

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“…A total of 40 healthy male albino Wistar rats (6-8 weeks old; 230-300 g) were purchased from the Animal Experiment Center of Dalian Medical University (Dalian, China) and maintained under standard conditions (22±2˚C with a 12-h dark-light cycle) and experiments were conducted in accordance with the Guide for the Care and Use of Laboratory Animals adopted by the Institutional Animal Care and Use Committee (11). All rats were fed with regular chow and given free access to water.…”

Section: Animalsmentioning

confidence: 99%

Protective effect of thymoquinone improves cardiovascular function, and attenuates oxidative stress, inflammation and apoptosis by mediating the PI3K/Akt pathway in diabetic rats

Liu

2016

Molecular Medicine Reports

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Thymoquinone is the main active monomer extracted from black cumin and has anti‑inflammatory, antioxidant and anti‑apoptotic functions. However, the protective effects of thymoquinone on cardiovascular function in diabetes remain to be fully elucidated. The present study aimed to investigate the molecular mechanisms underling the beneficial effects of thymoquinone on the cardiovascular function in streptozotocin‑induced diabetes mellitus (DM) rats. Supplement thymoquinone may recover the insulin levels and body weight, inhibit blood glucose levels and reduce the heart rate in DM‑induced rats. The results indicated that the heart, liver and lung to body weight ratios, in addition to the blood pressure levels, were similar for each experimental group. Treatment with thymoquinone significantly reduced oxidative stress damage, inhibited the increased endothelial nitric oxide synthase protein expression and suppressed the elevation of cyclooxygenase‑2 levels in DM‑induced rats. In addition, thymoquinone significantly suppressed the promotion of tumor necrosis factor‑α and interleukin‑6 levels in the DM‑induced rats. Furthermore, administration of thymoquinone significantly reduced caspase‑3 activity and the promotion of phosphorylated‑protein kinase B (Akt) protein expression levels in DM‑induced rats. These results suggest that the protective effect of thymoquinone improves cardiovascular function and attenuates oxidative stress, inflammation and apoptosis by mediating the phosphatidylinositol 3‑kinase/Akt pathway in DM‑induced rats.

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Effects of paroxetine on spatial memory function and protein kinase C expression in a rat model of depression

Cited by 16 publications

References 11 publications

Intrinsic cellular chirality regulates left–right symmetry breaking during cardiac looping

Intrinsic cellular chirality regulates left–right symmetry breaking during cardiac looping

Effect of different charges of modified electroconvulsive seizure on the cognitive behavior in stressed rats: Effects of GluR1 phosphorylation and CaMKIIα activity

Protective effect of thymoquinone improves cardiovascular function, and attenuates oxidative stress, inflammation and apoptosis by mediating the PI3K/Akt pathway in diabetic rats

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