Effects of paraquat and glyphosate on steroidogenesis in gonads of the frog Rana esculenta in vitro

Quassinti, Luana; Maccari, Ennio; Murri, Oretta; Bramucci, Massimo

doi:10.1016/j.pestbp.2008.11.006

Cited by 23 publications

(14 citation statements)

References 36 publications

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“…Another study, which tested glyphosate but not glyphosate‐based herbicides, found that gonadal steroidogenesis in Pelophylax kl. esculentus was not affected by glyphosate but was by another herbicide (paraquat) .…”

Section: Resultsmentioning

confidence: 89%

Questions concerning the potential impact of glyphosate‐based herbicides on amphibians

Wagner

Reichenbecher

Teichmann

et al. 2013

Enviro Toxic and Chemistry

142

107

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Use of glyphosate-based herbicides is increasing worldwide. The authors review the available data related to potential impacts of these herbicides on amphibians and conduct a qualitative meta-analysis. Because little is known about environmental concentrations of glyphosate in amphibian habitats and virtually nothing is known about environmental concentrations of the substances added to the herbicide formulations that mainly contribute to adverse effects, glyphosate levels can only be seen as approximations for contamination with glyphosate-based herbicides. The impact on amphibians depends on the herbicide formulation, with different sensitivity of taxa and life stages. Effects on development of larvae apparently are the most sensitive endpoints to study. As with other contaminants, costressors mainly increase adverse effects. If and how glyphosate-based herbicides and other pesticides contribute to amphibian decline is not answerable yet due to missing data on how natural populations are affected. Amphibian risk assessment can only be conducted casespecifically, with consideration of the particular herbicide formulation. The authors recommend better monitoring of both amphibian populations and contamination of habitats with glyphosate-based herbicides, not just glyphosate, and suggest including amphibians in standardized test batteries to study at least dermal administration.

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Section: Resultsmentioning

confidence: 89%

Questions concerning the potential impact of glyphosate‐based herbicides on amphibians

Wagner

Reichenbecher

Teichmann

et al. 2013

Enviro Toxic and Chemistry

142

107

View full text Add to dashboard Cite

show abstract

“…It has been demonstrated that pesticides affect gonadal development and hormone production in amphibians; the herbicide paraquat has been found to affect steroidogenesis in gonads of Rana esculenta (Quassinti et al, 2009), and glyphosate formulations have been shown to induce abnormal gonad development including the induction of testicular ovarian follicles and intersex gonads (Howe et al, 2004). Altered testicular histology and decrease in circulating sex steroid levels were also reported by several studies in amphibians residing in agricultural areas (Reeder et al, '98, 2005; Hecker et al, 2004; Jooste et al, 2005; Mosconi et al, 2005; McDaniel et al, 2008).…”

mentioning

confidence: 99%

Survival, development, and gonadal differentiation in Rana dalmatina chronically exposed to chlorpyrifos

et al. 2011

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Chlorpyrifos is an organophosphate pesticide among the most widely used in the world, which is suspected to be an endocrine-disrupting compound. To determine the capacity of chlorpyrifos to affect gonadal differentiation in Rana dalmatina, tadpoles were exposed to this pesticide during larval development until completion of metamorphosis at ecologically relevant concentrations (0.025 and 0.05 mg/L). No effects of chlorpyrifos exposure on survival, development, or metamorphosis were observed. After a 1 month metamorphosis, the gonadal phenotype was determined by gross morphology and histological examination. Morphological and histological analysis revealed normal ovaries or testes in froglets belonging to control group, whereas testes from several froglets exposed to chlorpyrifos were interspersed with testicular oocytes in histological sections. Chlorpyrifos exposure during the entire larval period did not affect sex ratio, but reduced the percentage of males with histologically normal testes. The findings suggest that chlorpyrifos exposure has significant effects on gonadal differentiation in some animals by inducing an intersex condition and alterations to testicular morphology, and that R. dalmatina is sensitive to endocrine disruption. Thereby, this study provides evidence that the ecologically relevant concentrations of chlorpyrifos, although not adversely affect the survival, development, or metamorphosis, may interfere with sex differentiation and reproductive development of R. dalmatina via endocrine-disrupting mechanisms.

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“…However, other herbicides used in this area also showed potential reproductive effects. Paraquat and glyphosate were found to have impacts on reproductive system of Rana esculenta [18]. Glyphosate alone was also toxic to tadpole of Rhinella arenarum [28] and could increase stress in many frog species [17].…”

Section: Gravimetric Parameters Of the Rice Frogsmentioning

confidence: 99%

“…Most of pesticides used in this area include herbicides namely atrazine, glyphosate and paraquat. Atrazine is a known endocrine-disrupting chemicals that can affect on reproductive system of amphibians [12][13][14][15][16] while glyphosate and paraquat are also found to show similar adverse effect on amphibians [17,18]. Since these herbicides have been intensively used in agricultural areas of Nan Province, the frogs that lived in these areas were unavoidably exposed to the herbicides.…”

Section: Introductionmentioning

confidence: 99%

Morphometric and Gravimetric Parameters of the Rice Frog <i>Fejervarya limnocharis</i> Living in Areas with Different Agricultural Activity

Thammachoti¹,

Khonsue²,

Kitana³

et al. 2012

JEP

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Use of agrochemicals in agricultural activity can lead to environmental contamination and affect non-target organisms including amphibians. In northern part of Thailand, there are many areas where agrochemicals, specifically herbicides, have been used for a long time. In this study, the rice frog Fejervarya limnocharis was used as a sentinel species to monitor potential relationship between agricultural activity, in form of herbicide utilization, and altered morphometric and gravimetric parameters. Frogs were field-collected on monthly basis during July 2010-June 2011 from a paddy field with intensive herbicide usage and a reference organic paddy field with no history of herbicide usage. Frogs were subjected to morphometry and gravimetry of liver, kidney, gonad and body. The results showed that condition factor of frogs in the contaminated site were significantly lower than those in the reference site, indicating potential impact on overall health. The gravimetric results showed that liver weight of frogs from the contaminated site were significantly higher than those in the reference site, indicating potential exposure to xenobiotics. There was no significant difference in kidney and testicular weight between these two sites. However, the frog from the impacted site had significantly higher ovarian weight than those from the reference site, indicating potential exposure to estrogenic substance. Overall, the results of this study could be used as early warnings of environmental health problems for other vertebrates living near the agricultural areas including human.

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Effects of paraquat and glyphosate on steroidogenesis in gonads of the frog Rana esculenta in vitro

Cited by 23 publications

References 36 publications

Questions concerning the potential impact of glyphosate‐based herbicides on amphibians

Questions concerning the potential impact of glyphosate‐based herbicides on amphibians

Survival, development, and gonadal differentiation in Rana dalmatina chronically exposed to chlorpyrifos

Morphometric and Gravimetric Parameters of the Rice Frog <i>Fejervarya limnocharis</i> Living in Areas with Different Agricultural Activity

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Effects of paraquat and glyphosate on steroidogenesis in gonads of the frog Rana esculenta in vitro

Cited by 23 publications

References 36 publications

Questions concerning the potential impact of glyphosate‐based herbicides on amphibians

Questions concerning the potential impact of glyphosate‐based herbicides on amphibians

Survival, development, and gonadal differentiation in Rana dalmatina chronically exposed to chlorpyrifos

Morphometric and Gravimetric Parameters of the Rice Frog &lt;i&gt;Fejervarya limnocharis&lt;/i&gt; Living in Areas with Different Agricultural Activity

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Product

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Morphometric and Gravimetric Parameters of the Rice Frog <i>Fejervarya limnocharis</i> Living in Areas with Different Agricultural Activity