Effects of pair-housing pubertal and adult male and female mice on LPS-induced age-dependent immune responses: A potential role for the gut microbiota

Murray, E. Selkirk; Butcher, James; Kearns, Madeleine May; Lamba, Sanjeevani; Liang, Jacky; Stintzi, Alain; Ismail, Nafissa

doi:10.1016/j.bbi.2023.03.009

Cited by 6 publications

(3 citation statements)

References 42 publications

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“…Metagenomic DNA was extracted from feces and cecal scrapings using the MP FastDNA SPIN kit and FastPrep-24 instrument (MP Biomedicals, Santa Ana, CA, USA), as previously described [ 34 ]. Briefly, samples were subjected to two rounds of mechanical lysis at 6 m/s for 40 s, with cooling on ice for at least 5 min between lysis rounds, followed by the standard manufacturer’s protocol.…”

Section: Methodsmentioning

confidence: 99%

“…V6-16S rRNA amplicon libraries were constructed and sequenced as previously described, with samples randomly distributed between sequencing libraries and selected samples sequenced multiple times to control for potential batch effects [ 34 ]. Briefly, the V6 hypervariable region of the extracted metagenomic DNA was amplified in the PCR reaction and each sample was pooled at equal masses.…”

Section: Methodsmentioning

confidence: 99%

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Glycomacropeptide as an Efficient Agent to Fight Pathophysiological Mechanisms of Metabolic Syndrome

Sauvé,

Feldman,

Sané

et al. 2024

Nutrients

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There is currently a growing interest in the use of nutraceuticals as a means of preventing the development of complex diseases. Given the considerable health potential of milk-derived peptides, the aim of this study was to investigate the protective effects of glycomacropeptide (GMP) on metabolic syndrome. Particular emphasis was placed on the potential mechanisms mitigating cardiometabolic disorders in high-fat, high-fructose diet-fed mice in the presence of GMP or Bipro, an isocaloric control. The administration of GMP for 12 weeks reduced obesity, hyperglycemia and hyperinsulinemia caused by a high-fat, high-fructose diet, resulting in a decline in insulin resistance. GMP also lessened systemic inflammation, as indicated by decreased circulating inflammatory cytokines. In the intestinal and hepatic tissues, GMP improved homeostasis by increasing insulin sensitivity and attenuating high-fat, high-fructose-induced inflammation, oxidative stress and endoplasmic reticulum stress. Biochemical and histological analyses revealed improved hepatic steatosis and fatty acid composition in the livers of high-fat, high-fructose diet-fed mice treated with GMP compared to Bipro. A trend toward a decrease in bile acids without any marked changes in intestinal microbiota composition characterized GMP-treated animals compared to those administered Bipro. GMP offers considerable potential for fighting metabolic syndrome-related components and complications given its beneficial effects on risk factors such as inflammation, oxidative stress and endoplasmic reticulum stress without involving the intestinal microbiota.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Glycomacropeptide as an Efficient Agent to Fight Pathophysiological Mechanisms of Metabolic Syndrome

Sauvé,

Feldman,

Sané

et al. 2024

Nutrients

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“…Pubertal development in mice is similar to that of humans ( Blaustein et al, 2016 ). In female mice, puberty begins with vaginal opening as the first sign of ovarian activity at approximately postnatal (PND) day 30 ( Kane and Ismail, 2017 ; Murray et al, 2023 ). In males, puberty typically begins with preputial separation around PND 42 ( Kane and Ismail, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Enduring sex-dependent implications of pubertal stress on the gut-brain axis and mental health

Dworsky-Fried,

Tchida,

Krnel

et al. 2024

Front. Behav. Neurosci.

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The gut-brain axis (GBA) is a network responsible for the bidirectional communication between the central nervous system and the gastrointestinal tract. This multifaceted system is comprised of a complex microbiota, which may be altered by both intrinsic and extrinsic factors. During critical periods of development, these intrinsic and extrinsic factors can cause long-lasting sex-dependent changes in the GBA, which can affect brain structure and function. However, there is limited understanding of how the GBA is altered by stress and how it may be linked to the onset of mental illness during puberty. This article reviews current literature on the relationships between the GBA, the effects of stress during puberty, and the implications for mental health.

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The enduring effects of antimicrobials and lipopolysaccharide on the cellular mechanisms and behaviours associated with neurodegeneration in pubertal male and female CD1 mice

Esposito,

Dubé-Zinatelli,

Gandelman

et al. 2024

Neuroscience

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Effects of pair-housing pubertal and adult male and female mice on LPS-induced age-dependent immune responses: A potential role for the gut microbiota

Cited by 6 publications

References 42 publications

Glycomacropeptide as an Efficient Agent to Fight Pathophysiological Mechanisms of Metabolic Syndrome

Glycomacropeptide as an Efficient Agent to Fight Pathophysiological Mechanisms of Metabolic Syndrome

Enduring sex-dependent implications of pubertal stress on the gut-brain axis and mental health

The enduring effects of antimicrobials and lipopolysaccharide on the cellular mechanisms and behaviours associated with neurodegeneration in pubertal male and female CD1 mice

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