Effects of P deficiency on assimilation and transport of nitrate and phosphate in intact plants of castor bean (<i>Ricinus communis</i>L.)

Jeschke, W. Dieter; Kirkby, Ernest A.; Peuke, Andreas D.; Pate, John S.; Hartung, Wolfram

doi:10.1093/jxb/48.1.75

Cited by 139 publications

(100 citation statements)

References 35 publications

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“…For instance, field experiments have shown that cotton plants with adequate K fertilization had increased efficiency of N fertilizer use and N utilization (Pettigrew and Meredith, 1997). Furthermore, it has been reported that P deficiency led to severe inhibition of nitrate uptake, nitrate transport into the xylem, and nitrate reduction in castor bean roots (Jeschke et al, 1997), and decreases in plant N and K accumulation (Soraya et al, 1996). Finally, when N-starved barley plants were transferred to solutions containing 0.2 mm KNO 3 , K ϩ uptake was increased immediately; in contrast, uptake of nitrate increased more slowly, matching the rate of K ϩ uptake after 4 h (Tischner, 1990).…”

Section: Nitrate-induced Genes In Tomato Rootsmentioning

confidence: 99%

Nitrate-Induced Genes in Tomato Roots. Array Analysis Reveals Novel Genes That May Play a Role in Nitrogen Nutrition,

2001

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A subtractive tomato (Lycopersicon esculentum) root cDNA library enriched in genes up-regulated by changes in plant mineral status was screened with labeled mRNA from roots of both nitrate-induced and mineral nutrient-deficient (Ϫnitrogen [N], Ϫphosphorus, Ϫpotassium [K], Ϫsulfur, Ϫmagnesium, Ϫcalcium, Ϫiron, Ϫzinc, and Ϫcopper) tomato plants. A subset of cDNAs was selected from this library based on mineral nutrient-related changes in expression. Additional cDNAs were selected from a second mineral-deficient tomato root library based on sequence homology to known genes. These selection processes yielded a set of 1,280 mineral nutrition-related cDNAs that were arrayed on nylon membranes for further analysis. These high-density arrays were hybridized with mRNA from tomato plants exposed to nitrate at different time points after N was withheld for 48 h, for plants that were grown on nitrate/ammonium for 5 weeks prior to the withholding of N. One hundred-fifteen genes were found to be up-regulated by nitrate resupply. Among these genes were several previously identified as nitrate responsive, including nitrate transporters, nitrate and nitrite reductase, and metabolic enzymes such as transaldolase, transketolase, malate dehydrogenase, asparagine synthetase, and histidine decarboxylase. We also identified 14 novel nitrate-inducible genes, including: (a) water channels, (b) root phosphate and K ϩ transporters, (c) genes potentially involved in transcriptional regulation, (d) stress response genes, and (e) ribosomal protein genes. In addition, both families of nitrate transporters were also found to be inducible by phosphate, K, and iron deficiencies. The identification of these novel nitrate-inducible genes is providing avenues of research that will yield new insights into the molecular basis of plant N nutrition, as well as possible networking between the regulation of N, phosphorus, and K nutrition.Nitrogen (N) is the essential mineral element required in the greatest amount in plants, comprising 1.5% to 2% of plant dry matter and approximately 16% of total plant protein (Frink et al., 1999). Thus, N availability is a major limiting factor for plant growth and crop production. Plant can utilize a wide range of N species including volatile ammonia (NH 3 ), nitrogen oxides (NO x ), mineral N (NO 3 Ϫ and NH 4 ϩ ), and organic N (amino acids, peptides, etc.; von Wirén et al., 1997). However, in most agricultural soils, nitrate (NO 3 Ϫ ) is the most important source of N (Crawford and Glass, 1998;Hirsch and Sussman, 1999). Because of the high N requirements for crop plants, N fertilization is a major worldwide agricultural investment, with 80 million metric tons of N fertilizers (as nitrate and/or ammonium) applied annually (Frink et al., 1999). There are also negative environmental consequences for the extensive use of N fertilizers in crop production because agricultural crops only retain about two-thirds of the applied N, and the unabsorbed N can subsequently leach into and contaminate water supplies (Frink et al., ...

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Section: Nitrate-induced Genes In Tomato Rootsmentioning

confidence: 99%

Nitrate-Induced Genes in Tomato Roots. Array Analysis Reveals Novel Genes That May Play a Role in Nitrogen Nutrition,

2001

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“…PAE is considered to be a major component of overall P efficiency (Ozturk et al, 2005;Ismail et al, 2007;Ramaekers et al, 2010), but PUE can influence PAE. P supply to plants is often fluctuating; therefore, P remobilization within the plant is critical for plant survival (Drew and Saker, 1984;Marschner and Cakmak, 1986;Jeschke et al, 1997;Vance et al, 2003;Huang et al, 2008). Remobilization and translocation of P from shoots to roots can lead to a larger root system, and hence greater exploitation of limited soil P resources for more P. However, the effect of PUE and simultaneous carbohydrate partitioning on PAE (Hermans et al, 2006;Liao et al, 2008) is not clear (Manske et al, 2001;Wang et al, 2010).…”

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confidence: 99%

Phosphate Utilization Efficiency Correlates with Expression of Low-Affinity Phosphate Transporters and Noncoding RNA, IPS1, in Barley

et al. 2011

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Genetic variation in phosphorus (P) efficiency exists among wheat (Triticum aestivum) and barley (Hordeum vulgare) genotypes, but the underlying mechanisms for the variation remain elusive. High-and low-affinity phosphate (Pi) PHT1 transporters play an indispensable role in P acquisition and remobilization. However, little is known about genetic variation in PHT1 gene expression and association with P acquisition efficiency (PAE) and P utilization efficiency (PUE). Here, we present quantitative analyses of transcript levels of high-and low-affinity PHT1 Pi transporters in four barley genotypes differing in PAE. The results showed that there was no clear pattern in the expression of four paralogs of the high-affinity Pi transporter HvPHT1;1 among the four barley genotypes, but the expression of a low-affinity Pi transporter, HvPHT1;6, and its close homolog HvHPT1;3 was correlated with the genotypes differing in PUE. Interestingly, the expression of HvPHT1;6 and HvPHT1;3 was correlated with the expression of HvIPS1 (for P starvation inducible; noncoding RNA) but not with HvIPS2, suggesting that HvIPS1 plays a distinct role in the regulation of the low-affinity Pi transporters. In addition, high PUE was found to be associated with high root-shoot ratios in low-P conditions, indicating that high carbohydrate partitioning into roots occurs simultaneously with high PUE. However, high PUE accompanying high carbon partitioning into roots could result in low PAE. Therefore, the optimization of PUE through the modification of low-affinity Pi transporter expression may assist further improvement of PAE for low-input agriculture systems.

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“…Mature leaves export nitrogen and phosphorous to other organs of plants (e.g., Aerts, 1996;Killingbeck, 1996;Jeschke et al, 1997;Hörtensteiner and Feller, 2002;Masclaux-Daubresse et al, 2010;Brant and Chen, 2015). In particular, plants resorb and remobilize essential nutrients to storage tissues in stems and roots during leaf senescence.…”

Section: Potential Mechanisms For the Observed Intra-plant δ 15 N-n Amentioning

confidence: 99%

The interaction between nitrogen and phosphorous is a strong predictor of intra-plant variation in nitrogen isotope composition in a desert species

Zhang

et al. 2017

Biogeosciences

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Abstract. Understanding intra-plant variations in δ 15 N is essential for fully utilizing the potential of δ 15 N as an integrator of the terrestrial nitrogen (N) cycle and as an indicator of the relative limitation of N and phosphorous (P) on plant growth. Studying such variations can also yield insights into N metabolism by plant as a whole or by specific organs. However, few researchers have systematically evaluated intra-plant variations in δ 15 N and their relationships with organ nutrient contents. We excavated whole plant architectures of Nitraria tangutorum Bobrov, a C 3 species of vital regional ecological importance, in two deserts in northwestern China. We systematically and simultaneously measured N isotope ratios and N and P contents of different parts of the excavated plants. We found that intra-plant variations in δ 15 N of N. tangutorum were positively correlated with corresponding organ N and P contents. However, it was the N × P interaction, not N and P individually or their linear combination, that was the strongest predictor of intra-plant δ 15 N. Additionally, we showed that root δ 15 N increased with depth into soil, a pattern similar to profiles of soil δ 15 N reported by previous studies in different ecosystems. We hypothesized that the strong positive intra-plant δ 15 N-N and P relationships are caused by three processes acting in conjunction: (1) N and P content-driven fractionating exchanges of ammonia between leaves and the atmosphere (volatilization) during photorespiration, (2) resorption and remobilization of N and P from senescing leaves, and (3) mixture of the retranslocated foliar N and P with existing pools in stems and roots. To test our hypothesis, future studies should investigate plant N volatilization and associated isotope fractionation and intra-plant variations in δ 15 N in different species across ecosystems and climates.

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Effects of P deficiency on assimilation and transport of nitrate and phosphate in intact plants of castor bean (Ricinus communisL.)

Cited by 139 publications

References 35 publications

Nitrate-Induced Genes in Tomato Roots. Array Analysis Reveals Novel Genes That May Play a Role in Nitrogen Nutrition,

Nitrate-Induced Genes in Tomato Roots. Array Analysis Reveals Novel Genes That May Play a Role in Nitrogen Nutrition,

Phosphate Utilization Efficiency Correlates with Expression of Low-Affinity Phosphate Transporters and Noncoding RNA, IPS1, in Barley

The interaction between nitrogen and phosphorous is a strong predictor of intra-plant variation in nitrogen isotope composition in a desert species

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