Effects of ozone water irrigation and spraying on physiological characteristics and gene expression of tomato seedlings

Abstract

Tomato seedlings were used as experimental materials and treated with 1.0, 2.0, 3.0, and 4.0 mg/L ozone water irrigation and 0.2, 0.4, 0.6, and 0.8 mg/L ozone water spray treatments. Indexes including the malondialdehyde (MDA) content, superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT), activities, soil and plant analysis development (SPAD) value, and nitrogen content of leaves were measured. Furthermore, the expression of antioxidant enzyme, chlorophyll synthesis and nitrogen absorption genes was analyzed after optimal ozone water treatment. The results showed that the activities of antioxidant enzymes in tomato leaves were significantly increased, and the MDA content in tomato leaves was significantly reduced by ozone water irrigation and spray treatment, which indicated that ozone water treatment can significantly improve the stress tolerance of tomato seedlings. Ozone water irrigation and spraying could also significantly increase the leaf SPAD value and nitrogen content of tomato seedlings, and the optimal concentrations of ozone water irrigation and spraying were 3.0 mg/L and 0.6 mg/L, respectively. The effect of ozone water irrigation on improving the physiological characteristics of tomato seedlings was better than that of spraying. After treatment with the optimal concentration of ozone water, the relative expression of antioxidant enzyme, chlorophyll synthesis, and nitrogen absorption genes was significantly increased, and the maximum expression level was reached at 12 h. In addition, ozone water irrigation could promote the expression of genes more than ozone water spraying, which was consistent with the improvements in the physiological characteristics of the tomato seedlings.

Genome-Wide Transcriptomic and Proteomic Exploration of Molecular Regulations in Quinoa Responses to Ethylene and Salt Stress

“…In this research, 4-week-old quinoa seedlings of 'NL-6 treated with water, 300 mM NaCl, or 300 mM NaCl with 100 µM ACC for 2-3 d were used for examination of physiological indexes. The nitrogen content and relative level of total chlorophyll were measured by PJ-4N plant nutrition analyzer, and the relative permeability of cell membrane, damage rate of leaves, malondialdehyde (MDA) content, soluble sugar level, and SOD activity were analyzed as previously described [35]. Three independent biological replicates and three technical replicates were used in the experiments.…”

Appropriate N application alleviates growth inhibition of okra (Abelmoschus esculentus L. Moench) caused by fluctuating soil moisture

“…Malondialdehyde is a byproduct of cell membrane lipid peroxidation, and its concentration re ects the level of adversity-induced plant stress (Xu et al, 2021). As shown in Fig.…”

Effect of ozone sterilization on controlling leaf mildew and gray mold tomato disease in a glasshouse and its influence on other growth parameters

“…As an ideal green strong oxidant, ozone has no residue, no secondary pollution, does not produce toxic compounds, strong oxidation, fast reaction, can oxidize microbial cells, resulting in microbial death, ozone sterilization provides an effective solution for the prevention and control of tomato disease facilities. [10][11][12] In recent years, domestic and foreign experts and scholars have carried out extensive research on ozone control of tomato diseases. Domestic scholars such as Li et al have carried out tests for the control effect of ozone functional water on gray mold, leaf mildew, early blight, late blight and whitefly of tomato in the glasshouse, and the control effect was more than 80%.…”

“…As an ideal green strong oxidant, ozone has no residue, no secondary pollution, does not produce toxic compounds, strong oxidation, fast reaction, can oxidize microbial cells, resulting in microbial death, ozone sterilization provides an effective solution for the prevention and control of tomato disease facilities 10‐12 . In recent years, domestic and foreign experts and scholars have carried out extensive research on ozone control of tomato diseases.…”