Effects of ozone treatment on cell growth and ultrastructural changes in bacteria.

Thanomsub, Benjamas; Anupunpisit, Vipavee; Chanphetch, Silchai; Watcharachaipong, Thanomrat; Poonkhum, Raksawan; Srisukonth, Chuda

doi:10.2323/jgam.48.193

Cited by 154 publications

(122 citation statements)

References 28 publications

(24 reference statements)

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“…Ozone was detected spectrophotometrically in the discharge associated with this plasma discharge. Thanomsub et al [45] reported that levels of ozone less than 1 ppm can have bactericidal properties. While the results from that study indicate ozone at low concentrations can have antimicrobial properties, we believe that it does not contribute significantly to the overall germicidal capability of the nonthermal plasma discharge independently.…”

Section: Discussionmentioning

confidence: 99%

Characterization, Properties and Applications of Nonthermal Plasma: A Novel Pulsed-Based Option

Ferrell¹,

Aleks²,

Galov³

et al. 2013

J Biotechnol Biomaterial

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The glow discharge from a novel spark-based nonthermal plasma generator is described and characterized using spectrophotometry, radiometry and gas detectors. Spectral information identified ultraviolet radiation within the plasma discharge. High levels of reactive oxygen and nitrogenous species were also detected within the analyzed plasma. The plasma discharge is found to have a significant germicidal effect on Gram-positive bacteria, independent of temperature increase. The ultraviolet radiation and ionized gaseous species are found to contribute to this observed effect, synergistically. Plasma filtration reduced germicidal survivability significantly. The liquid environment in which bacterial cells were exposed to the nonthermal plasma also was important, when determining the efficacy of nonthermal plasma, as a germicidal agent. Staphylococcus aureus cultures exposed to nonthermal plasma for two minutes within low nutrient sources, such as water or phosphate-buffered saline, had very low survival. Cultures exposed to the same plasma interval in nutrient rich liquids tolerated the plasma exposure better, and survived at higher numbers. This plasma discharge is highly bactericidal, due to the high concentrations of known antimicrobial agents, but its effectiveness is subject to a synergistic effect, that depends upon the surrounding environment.

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Section: Discussionmentioning

confidence: 99%

Characterization, Properties and Applications of Nonthermal Plasma: A Novel Pulsed-Based Option

Ferrell¹,

Aleks²,

Galov³

et al. 2013

J Biotechnol Biomaterial

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“…Either the outer lipid membrane in Gram-negative bacteria or the peptidoglycan cell wall in Gram-positive bacteria is the first structure to be oxidized by the oxygen atom. After the membrane alterations, the successive bacterial inactivation is caused by a lysis reaction (Thanomsub et al 2002). The aim of this study was to evaluate first the direct effect of ozone as a gas in saline on bacterial survival either when bacteria are suspended in both pure physiological solutions or after addition of human plasma at various concentrations or in blood.…”

Section: Introductionmentioning

confidence: 99%

How much ozone bactericidal activity is compromised by plasma components?

Burgassi

Zanardi

Travagli

et al. 2009

Journal of Applied Microbiology

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Aims: Evaluation of bactericidal effect of different concentrations of ozone\ud when used (a) as a gas, or (b) dissolved in saline. The addition of hydrogen\ud peroxide or 4-hydroxynonenal dissolved in saline was also tested, as well as the\ud effect of human plasma.\ud Methods and Results: Staphylococcus aureus, methicillin-resistant Staph. aureus\ud (MRSA), and Pseudomonas aeruginosa, suspended in their culture media were\ud tested. While all bacteria suspended in protein-free saline were killed at high\ud ozone concentrations, they survived when as little as 5% human plasma was\ud present. Hydrogen peroxide was 100-fold less active than ozone and needed to\ud remain in contact with bacteria for at least 60 min. 4-hydroxynonenal\ud (2 lmol l)1) was inhibitory for proliferation of both Staph. aureus and MRSA,\ud but not for Ps. aeruginosa.\ud Conclusions: Ozone and the cascade of its derivative products are potent bactericidal\ud agents, but even small amounts of human plasma, hence of hydro- and\ud liposoluble antioxidants, in bacterial suspensions inhibit oxidation and protect\ud bacteria.\ud Significance and Impact of the Study: Any substantial in vivo cytocidal effect\ud of ozone and its derivatives can be excluded. On the other hand, topical and\ud continuous action of various ozone preparations remains valuable in a variety\ud of skin and mucosal infections

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“…The oxidation causes cell lysis and prevents bacterial growth (Santos et al, 2007;Silva et al, 2009). Thanomsub et al (2002) found that the ozone treatment was able to modify and destroy the structure of Gram-positive and Gram-negative bacteria. After 30 minutes of ozone exposure, the number of bacteria in cultures of 10 3 , 10 4 , and 10 5 CFU/ml decreased.…”

Section: Discussionmentioning

confidence: 99%

“…However, at higher bacterial loads (concentrations of 10 6 and 10 7 CFU/ml), there was a gradual decline in the survival of the cells, but the ozone was not effective against all bacterial cells even after a longer treatment period (150 minutes). Adequate concentrations of ozone can effectively result in antimicrobial activity by destroying the bacterial cell membrane and resulting in intracellular leakage and cell lysis (Thanomsub et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

Effectiveness of ozonated water in the reprocessing of blood dialyzers

Canada

Abelan

Zângaro

et al. 2014

Rev. Bras. Eng. Bioméd.

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Introduction: Ozone is a potent antibacterial agent. Because ozone oxidizes organic material, it directly attacks microorganisms resulting in safe, fast and economical sterilization at low temperatures. This study evaluated the effi cacy of ozonated water in the reprocessing of dialyzers obtained from a hemodialysis service in São José do Rio Preto. Methods: A total of 23 blood dialyzers were collected at the conclusion of the morning hemodialysis shift. The equipment was collected after the maximum number of reuses, with the last disinfection performed with purifi ed water (obtained by reverse osmosis and subsequent reverse ultrafi ltration). The number and species of microorganisms in the dialyzers were evaluated before and after treatment with ozonated water. The ozonation of sterile deionized water was achieved by direct contact between the water and the ozone generating equipment. Results: The mean number of microorganisms before sterilization was 1,47.10 9 colony forming units per ml (CFU/ml). After treatment with ozonated water, the number of microorganisms was 4,80.10 1 CFU/ml. Conclusion: Ozonated water is an effective decontaminant for most blood dialyzers.

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Effects of ozone treatment on cell growth and ultrastructural changes in bacteria.

Cited by 154 publications

References 28 publications

Characterization, Properties and Applications of Nonthermal Plasma: A Novel Pulsed-Based Option

Characterization, Properties and Applications of Nonthermal Plasma: A Novel Pulsed-Based Option

How much ozone bactericidal activity is compromised by plasma components?

Effectiveness of ozonated water in the reprocessing of blood dialyzers

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