Effects of Oxytocin, Prostaglandin F&lt;sub&gt;2α&lt;/sub&gt; and Prostaglandin E&lt;sub&gt;2&lt;/sub&gt; on Intracellular Free Calcium Concentrations of Longitudinal Muscle Cells Isolated from Term Pregnant Rat Myometrium

Kawarabayashi, Tatsuhiko; Shojo, Hideki; Tsukamoto, Takuji

doi:10.1159/000291842

Cited by 5 publications

(4 citation statements)

References 5 publications

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“…The L-type Ca 2+ channel is well known to be a major pathway for Ca 2+ influx during uterine contractions. 10 –13 Its expression was increased by 2.5-fold in the odontogenic infection model mice in the present study, suggesting enhanced electrical excitability of the myometrial cells. These findings support that odontogenic infection in mice is useful as a model of preterm delivery induced by inflammation.…”

Section: Discussionsupporting

confidence: 63%

“…The main source of Ca 2þ is thought to be Ca 2þ influx through L-type Ca 2þ channels and receptor-operated nonselective cation channels (NSCCs). [10][11][12][13] In addition, myometrial NSCCs play an important role in changing the membrane potential to induce spontaneous uterine contractions. We previously identified that the purinergic receptor P2X7 is one of the myometrial NSCCs in the rat uterus.…”

Section: Introductionmentioning

confidence: 99%

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Enhanced Expression of Contractile-Associated Proteins and Ion Channels in Preterm Delivery Model Mice With Chronic Odontogenic Porphyromonas Gingivalis Infection

et al. 2016

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Inflammation and infection have been reported to induce preterm delivery. We have studied the relationship between inflammation and various ion channels, including the L-type Ca(2+) channel and P2X7 receptor, during acute inflammation of the pregnant rat uterus induced by lipopolysaccharides. Recently, we found that mice with odontogenic Porphyromonas gingivalis (P.g, an important odontogenic pathogen) infection delivered at day 18.3 of gestation (vs. day 20.5 in normal mice). The purpose of this study was to investigate the expression of myometrial contractile-associated proteins inducing contractions and confirm that these mice are useful as a model for preterm delivery induced by chronic inflammation. We examined the expression of the oxytocin receptor, connexin 43, prostaglandin F receptors, L-type Ca(2+) channel, and P2X7 receptor in the myometrium at day 18 of gestation by real-time PCR and western blot analyses. We also measured TNF-α and IL-1β levels in the blood serum, placenta, fetal membrane and myometrium on the same day. mRNA expression of the oxytocin receptor, connexin 43, prostaglandin F receptors, L-type Ca(2+) channel, and P2X7 receptor was elevated by 5.4, 3.2, 2.4, 2.5, and 1.7 fold, respectively, in the P.g-infected mice. Protein levels of the oxytocin receptor and connexin 43 also increased. Serum levels of TNF-α and IL-1β were elevated, showing that systemic inflammation continued during pregnancy. IL-1β levels in the placenta and fetal membrane also increased, suggesting inflammatory reactions were induced. Thus, mice with odontogenic infection may be useful as a model of chronic inflammation-induced preterm delivery.

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Section: Discussionsupporting

confidence: 63%

Section: Introductionmentioning

confidence: 99%

Enhanced Expression of Contractile-Associated Proteins and Ion Channels in Preterm Delivery Model Mice With Chronic Odontogenic Porphyromonas Gingivalis Infection

et al. 2016

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“…The best known uterine contracting agents, e.g. oxytocin and prostaglandins, lose their effects after removal of the extracellular Ca 2ϩ [19]. The smooth muscle contraction therefore depends greatly on the amount of extracellular Ca 2ϩ ions entering the cell.…”

Section: Discussionmentioning

confidence: 99%

“…The aim of the present study was to characterize the ability of such uterine tissues to contract in response to EFS in late pregnancy (days [15][16][17][18][19][20][21][22] in the rat. For this purpose, we set out to describe the contractility by choosing stimulation parameters necessary to elicit rhythmic contractions.…”

Section: Introductionmentioning

confidence: 99%

Characterization of late-pregnant rat uterine contraction via the contractility ratio in vitro Significance of α1-adrenoceptors

et al. 2001

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The aim of this study was to characterize the ability of late-pregnant (days 15-22) rat uterine tissue rings to contract in response to electric field stimulation in vitro. For this purpose, maximum rhythmic contractions were elicited by optimum choice of the period time and the pulse width, the two main parameters of electric field stimulation. In parallel, the plasma 17beta-estradiol and progesterone levels were determined. It was found that the contractility ratio, i.e. the quotient of the optimum pulse width and the period time, is a good parameter with which to express the contractility. The larger the contractility ratio, the better the ability to contract. Evaluation of the area under the curve did not furnish information relating to the contractility in this method. A very close correlation was observed between the contractility ratio and the quotient of the 17beta-estradiol and progesterone levels on different days, demonstrating that the in vitro ability characterized by the contractility ratio is in keeping with the physiological regularity. There was also a very close correlation between the contractility ratio and the quotient of the alpha1- and beta-adrenergic receptors, suggesting the main role of the numbers of alpha1-receptor in pregnant uterine contractility. It is believed that this is the first in vitro model to give a numerical measure concerning the ontogeny of uterine contractility in late pregnancy.

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The mechanism of honokiol-induced and magnolol-induced inhibition on muscle contraction and Ca 2+ mobilization in rat uterus

Chen

et al. 2003

Naunyn-Schmiedeberg's Archives of Pharmacology

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The effects of honokiol and magnolol extracted from the Magnolia officinalis on muscular contractile responses and intracellular Ca(2+) mobilization were investigated in the non-pregnant rat uterus. Honokiol and magnolol (1-100 micromol/l) were observed to inhibit spontaneous and uterotonic agonists (carbachol, PGF(2alpha), and oxytocin)-, high K(+)-, and Ca(2+) channel activator (Bay K 8644)-induced uterine contractions in a concentration-dependent manner. The inhibition rate of honokiol on spontaneous contractions appeared to be slower than that of magnolol-induced response. The time periods that were required for honokiol and magnolol, at 100 micromol/l, to abolish 50% spontaneous contractions were approximately 6 min. Furthermore, honokiol and magnolol at 10 micromol/l also blocked the Ca(2+)-dependent oscillatory contractions. Consistently, the increases in intracellular Ca(2+) concentrations ([Ca(2+)](i)) induced by PGF(2alpha) and high K(+) were suppressed by both honokiol and magnolol at 10 micromol/l. After washout of these treatments, the rise in [Ca(2+)](i) induced by PGF(2alpha) and high K(+) was still partially abolished. In conclusion, the inhibitory effects of honokiol and magnolol on uterine contraction may be mediated by blockade of external Ca(2+) influx, leading to a decrease in [Ca(2+)](i). Honokiol and magnolol may be considered as putative Ca(2+) channel blockers and be of potential value in the treatment of gynecological dysfunctions associated with uterine muscular spasm and dysmenorrhea.

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Effects of Oxytocin, Prostaglandin F<sub>2α</sub> and Prostaglandin E<sub>2</sub> on Intracellular Free Calcium Concentrations of Longitudinal Muscle Cells Isolated from Term Pregnant Rat Myometrium

Cited by 5 publications

References 5 publications

Enhanced Expression of Contractile-Associated Proteins and Ion Channels in Preterm Delivery Model Mice With Chronic Odontogenic Porphyromonas Gingivalis Infection

Enhanced Expression of Contractile-Associated Proteins and Ion Channels in Preterm Delivery Model Mice With Chronic Odontogenic Porphyromonas Gingivalis Infection

Characterization of late-pregnant rat uterine contraction via the contractility ratio in vitro Significance of α1-adrenoceptors

The mechanism of honokiol-induced and magnolol-induced inhibition on muscle contraction and Ca 2+ mobilization in rat uterus

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