Effects of oxytocin on the bovine corpus luteum of early pregnancy

Tan, G. J. S.; Tweedale, Rowan; Biggs, J. S. G.

doi:10.1530/jrf.0.0660075

Cited by 66 publications

(25 citation statements)

References 10 publications

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“…There must be some sort of communication between the cell types. One possible candidate for mediating this communication is oxytocin, since: (1) oxytocin has been shown to be present in large luteal cells (Rodgers, O'Shea, Findlay, Flint & Sheldrick, 1983b;Guldenaar, Wathes & Pickering, 1984;Sawyer & Moeller, 1985); (2) cloprostenol, an analogue of PGF-2a, has been shown to stimulate the release of oxytocin by the corpus luteum (Flint & Sheldrick, 1982); and (3) oxytocin has been reported to inhibit the ability of hCG to stimulate progesterone secretion by bovine (Tan, Tweedale & Biggs, 1982a) and human (Tan et al, 1982b) luteal tissue. However, some investigators have found no effect of oxytocin on progesterone secretion by human luteal tissue (Richardson & Masson, 1985).…”

Section: Introductionmentioning

confidence: 99%

Differential regulation by LH and prostaglandins of steroidogenesis in small and large luteal cells of the ewe

Schwall¹,

Sawyer²,

Niswender³

1986

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Section: Introductionmentioning

confidence: 99%

Differential regulation by LH and prostaglandins of steroidogenesis in small and large luteal cells of the ewe

Schwall¹,

Sawyer²,

Niswender³

1986

Reproduction

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“…Although these data suggest that luteal OT plays an important physiological role as an autocrine or paracrine regulator or both, a satisfactory explanation of the physiological role of OT in the CL has not been provided. In vitro models, which are mainly based on the incubation of dispersed luteal cells, have shown a variety of CL responses to OT in terms of progesterone production: a luteotropic effect [11], …”

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confidence: 99%

Progesterone Release of Bovine Corpus Luteum in Response to Oxytocin in Different Culture Systems.

Sakumoto

Ando

Okuda

1996

Journal of Reproduction and Development

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Abstract. The aim of the present study was to investigate the effect of oxytocin (OT) on progesterone secretion by bovine corpus luteum (CL) in different culture systems. When luteal cells were cultured for 1, 2, 4 and 15 h at a density of 6 × 10 4 cells/ml, OT (10 -7 M) did not affect progesterone secretion. On the other hand, bovine LH strongly stimulated progesterone secretion by the cells 2-15 h after incubation (P<0.01). In another experiment, a novel luteal slice culture system was used, in which luteal cells maintained cell-to-cell contact. Three slices (15-20 mg/a slice) from a CL were pre-incubated for 1 h, and then each slice was incubated for 1 h in a culture tube with a medium (3 ml) supplemented with and without bovine LH (100 ng/ml) or OT (10 -7 M). Slices were replaced every 1 h in a fresh medium during the 5 h incubation experiment. A 1-h stimulation with bovine LH and OT induced significant but different effects on the release of progesterone. OT acutely stimulated progesterone release at doses 10 -7 M (155%; P<0.05), and bovine LH significantly increased progesterone release during and after stimulation (223%; P<0.01). These data support the hypothesis that OT plays a role in modulating the mechanisms of progesterone secretion as an intraluteal regulator. Differences observed between the two systems might be due to the presence or absence of cell-to-cell contact. Key words: Oxytocin, Cell-to-cell contact, Corpus luteum, Bovine.(J. Reprod. Dev. 42 : 199-204, 1996) Accepted for publication: June 3, 1996 Correspondence: K. Okuda t is well known that oxytocin (OT) is synthesized and secreted by the corpus luteum (CL) a luteolytic effect [12], or no effect [13,14]. These different results obtained from in vitro studies seem to depend on the experimental model, e.g., shortor long-term culture of luteal cells, cell density and duration of stimulation with reagents. Recently, it has been reported that the communication between large and small luteal cells is very important for the function of the CL [15]. In addition, Miyamoto et al. [16] reported that OT stimulated progesterone production from bovine CL using an in vitro microdialysis system (MDS). In contrast to classical cell culture techniques, MDS maintains cell-to-cell contact as is the case in vivo. Thus, it is strongly suggested that cell-to-cell contact may be a requisite for the CL response to OT.The present study was conducted to investigate I in many species [1][2][3][4][5][6][7]. Moreover, the presence of OT receptors in the CL has been demonstrated in the cow [8], pig [9] and sheep [10]. Although these data suggest that luteal OT plays an important physiological role as an autocrine or paracrine regulator or both, a satisfactory explanation of the physiological role of OT in the CL has not been provided. In vitro models, which are mainly based on the incubation of dispersed luteal cells, have shown a variety of CL responses to OT in terms of progesterone production: a luteotropic effect [11],

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“…Firstly, oxytocin concentrations > 100 ng/ml have generally been used to show inhibition in vitro, while low doses have occasionally given stimu¬ lation rather than inhibition (Tan et al, 1982a, b). The failure of Gilbert et al (1989) to show an effect in cattle may be explained by the 70-500 pg oxytocin/ml plasma achieved in their experiments compared with~1 µg/ml of incubation medium used by Tan et al (1982a). Schams (1989) observed effects at much lower doses than Tan et ai (1982a), but, curiously, the magnitude of the inhibition was independent of oxytocin concentration.…”

Section: Discussionmentioning

confidence: 91%

Effect of intra-ovarian infusion of oxytocin on plasma progesterone concentrations in pregnant ewes

Sernia

Gemmell²,

Thomas³

1991

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Summary. The function of oxytocin receptors in the corpus luteum of pregnant ewes was investigated by infusing saline or oxytocin (100 ng/min) into the utero\p=n-\ovarian artery of pregnant ewes (62 \m=+-\5 days, n = 12). During a 4-h infusion, plasma oxytocin (OT) concentration increased to 268 \ m=+-\ 80 pg OT/ml in the OT-infused group and remained unchanged at 2\m=.\5\m=+-\1\m=.\5 pg OT/ml in the saline-infused group. Progesterone concentration in jugular venous plasma (17 \m=+-\9 ng/ml) rapidly decreased during oxytocin infusion to 59 \m=+-\10% and 26 \ m=+-\ 9% of control at 1\m=.\5and 2 h, respectively; the utero\p=n-\ovarianvenous concentration of 64 \m=+-\38 ng/ml decreased by a similar magnitude during oxytocin infusion. Electron microscopy of corpora lutea, removed at the end of the experiments, showed no indication of luteolytic changes following oxytocin infusion. It was concluded that oxytocin markedly and rapidly reduces progesterone secretion in pregnant ewes.

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Effects of oxytocin on the bovine corpus luteum of early pregnancy

Cited by 66 publications

References 10 publications

Differential regulation by LH and prostaglandins of steroidogenesis in small and large luteal cells of the ewe

Differential regulation by LH and prostaglandins of steroidogenesis in small and large luteal cells of the ewe

Progesterone Release of Bovine Corpus Luteum in Response to Oxytocin in Different Culture Systems.

Effect of intra-ovarian infusion of oxytocin on plasma progesterone concentrations in pregnant ewes

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