Effects of Oxygen Supply on Electrical and Secretory Responses of Humorally Stimulated Acinar Cells in Isolated Rat Pancreas

Kanno, Tomio; Suga, Toshihiro; Yamamoto, Masao

doi:10.2170/jjphysiol.26.101

Cited by 40 publications

(24 citation statements)

References 20 publications

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“…produced a hyperpolarization of the acinar cells and increased the amylase output from the isolated rat pancreas (Kanno, 1972(Kanno, , 1974(Kanno, , 1975. These effects of Pz were enhanced when the pancreas was perfused with an oxygenated Krebs-Henseleit solution containing dog erythrocytes (Kanno et al 1976). The present experiments confirmed these effects during perfusion with the solution containing 0-5 m-u.…”

Section: Resultsmentioning

confidence: 99%

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The potentiating influences of insulin on pancreozymin‐induced hyperpolarization and amylase release in the pancreatic acinar cell.

Kanno¹,

Saito²

1976

The Journal of Physiology

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SUMARY1. Insulin (1 u./ml.) potentiated the release of amylase from isolated pancreas of rats perfused with erythrocyte-containing medium and stimulated by 0-5 m-u. pancreozymin (Pz)/ml., whereas the same concentration of insulin failed to potentiate the response evoked by 5 or 200 m-u. Pz/ml.2. Intracellular measurement of membrane potentials from the acinar cells of the same preparations showed that insulin (1 u./ml.) simultaneously potentiates the hyperpolarization and amylase release in response to 0-5 rn-u. Pz/ml.3. These effects of insulin on the Pz-induced responses were inhibited by ouabain (3 x 105 M).4. The results suggest that insulin, in the concentration studied, has a potentiating action on the activity of an electrogenic Na pump, which maintains the hyperpolarization and amylase release during continuous stimulation with Pz.5. Insulin also potentiated the Pz-induced amylase release in the rat pancreata in situ after vagotomy and ligature of the pyloric region.

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Section: Resultsmentioning

confidence: 99%

“…modified Krebs-Henseleit solution. Both preparations utilize the whole pancreas and a full description of the procedures has been given in a previous paper (Kanno et al 1976). The temperature of the preparation and perfusing solution was maintained at 37 0C by immersing the apparatus in a water-bath.…”

Section: Methodsmentioning

confidence: 99%

The potentiating influences of insulin on pancreozymin‐induced hyperpolarization and amylase release in the pancreatic acinar cell.

Kanno¹,

Saito²

1976

The Journal of Physiology

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“…The isolated perfused pancreas was prepared by the method of KANNO et al (1976). Under ether anesthesia, the superior mesenteric and coeliac arteries as inlets and the portal vein as an outlet were cannulated, and then perfused at about 2 ml/(min .…”

Section: Methodsmentioning

confidence: 99%

Secretory responses of hypertrophied rat pancreas induced by repeated oral administrations of a synthetic protease inhibitor.

Yonezawa

1983

Jpn.J.Physiol

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A dose of -(4-guanidinobenzoyloxy)phenylacetate methanesulfonate; 100 mg/kg body weight] was administered orally once a day for 30 days to a group of rats (FOY group). To the control group of rats, 10 ml/kg body weight of distilled water (a solvent of FOY-305) was administered in the same way. The FOY-305 administration induced hypertrophy of the pancreas : the increase in wet weight of the pancreas was significantly higher than the increase in DNA content. The administration also induced hyperpyknia in the FOY group as shown by the increased concentration of constituents (amylase, trypsinogen, and chymotrypsinogen) in the pancreas.The secretory responses of the exocrine pancreas to stimulation with cholecystokinin (CCK) were examined in vivo. The secretory responses induced by lowered doses of CCK in the FOY group were slightly greater than those in the control group, but the differences were not statistically significant. On the other hand, the responses induced by higher doses of CCK were significantly greater than those in the control group. The secretory responses to stimulation with CCK or acetylcholine (ACh) were examined further in vitro using an isolated perfused pancreas. Protein output induced by lower doses of the secretagogues (146-730 pM CCK or 10 nM ACh) was similar in the control and the FOY groups, but the output induced by higher doses of the secretagogues (1,460 pM CCK or 30-100 nM ACh) in the FOY group was significantly larger than that in the control group.The present results provided evidence that the FOY-305-induced hypertrophy and hyperpyknia of the rat pancreas coincided with potentiation of secretory responses to higher doses of the secretagogues.

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“…Two alternative explanations were given (Dockray, 1972): the two hormones were acting on different cells, or the differences might be a reflexion of different rates of metabolism of CCK-PZ and secretin. To decide which of these explanations is acceptable, the isolated and perfused preparation of rat pancreas may provide a promising clue: the perfusion technique has been used successfully in simultaneous measurements of the pancreatic enzyme release and juice flow (Kanno, Suga & Yamamoto, 1976;Kanno & Nishimura, 1976;Kanno & Saito, 1976). The results of the present study show that pure natural CCK-PZ produces a marked increase both in amylase output and in juice flow which is mainly dependent upon the presence of Ca2+ in the perfusing medium, while synthetic secretin produces an increase in juice flow along with a slight increase in amylase output which is largely dependent on HC03-and only partly on Ca2+ in the perfusing medium.…”

Section: Introductionmentioning

confidence: 99%

Differentiation between the calcium‐dependent effects of cholecystokinin‐pancreaozymin and the bicarbonate‐dependent effects of secretin in exocrine secretion of the rat pancreas.

Kanno¹,

Yamamoto²

1977

The Journal of Physiology

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SUMMARY1. Differentiation between the secretary effects of pure natural cholecystokinin-pancreozymin (COK-PZ) and those of synthetic secretin was studied in the isolated pancreas of the rat perfused with a standard solution containing both Ca2+ and HCO3-, a Oa2+-deficient solution, or a HC-G-deficient solution.2. Secretin induced a dose-dependent increase in the flow of pancreatic juice and a slight but definite increase in amylase output which was also dependent upon the dose of secretin.3. The increase in the flow of pancreatic juice, induced by continuous stimulation with secretin (5 Ivy dog m-u./ml.), was completely abolished during perfusion with a HCO3--deficient solution, but was only slightly suppressed during perfusion with a Ca2+-deficient solution.4. The increase in flow, induced by continuous stimulation with CCK-PZ (5 Ivy dog m-u./ml.), was partly affected by the deprivation of HCO3-, but was strongly inhibited by the deprivation of Ca2+. The CCK-PZ-induced amylase output was not affected by the deprivation of HOG-, but was significantly inhibited by the deprivation of Ca2+.5. The present results favour the view that CCK-PZ acts on the acinar cells to increase both amylase output and juice flow, whereas secretin acts on centro-acinar and terminal duct cells to increase mainly juice flow.

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Effects of Oxygen Supply on Electrical and Secretory Responses of Humorally Stimulated Acinar Cells in Isolated Rat Pancreas

Cited by 40 publications

References 20 publications

The potentiating influences of insulin on pancreozymin‐induced hyperpolarization and amylase release in the pancreatic acinar cell.

The potentiating influences of insulin on pancreozymin‐induced hyperpolarization and amylase release in the pancreatic acinar cell.

Secretory responses of hypertrophied rat pancreas induced by repeated oral administrations of a synthetic protease inhibitor.

Differentiation between the calcium‐dependent effects of cholecystokinin‐pancreaozymin and the bicarbonate‐dependent effects of secretin in exocrine secretion of the rat pancreas.

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