Effects of oxygen plasma treatment on adipose-derived human mesenchymal stem cell adherence to poly(L-lactic acid) scaffolds

Abstract

Plasma treatment of substrate surfaces can be utilized to improve adhesion of cells to tissue-engineered scaffolds. The purpose of this study was to enhance cell adhesion to non-woven poly(L-lactic acid) (PLLA) scaffolds using oxygen plasma treatment to increase surface hydroxyl groups and thereby enhance substrate hydrophilicity. It was hypothesized that oxygen plasma treatment would increase the number of adipose-derived human mesenchymal stem cells (hMSCs) that adhered to melt-blown, non-woven PLLA scaffolds without affecting cell viability. The number of cells that adhered to the oxygen plasma-treated (10 min at 100 W) or untreated PLLA scaffolds was assessed at 2, 4, 8, 12, 24 and 48 h post-seeding via DNA analysis. Cell viability and morphology were also assessed at 2, 4, 8, 12 and 24 h post-seeding via a live/dead assay and hematoxylin staining, respectively. Oxygen plasma treatment decreased the contact angle of water from 75.6 degrees to 58.2 degrees , indicating an increase in the surface hydrophilicity of PLLA. The results of the DNA analysis indicated that there was an increased number of hMSCs on oxygen plasma treated scaffolds for two of the three donors. In addition, oxygen plasma treatment promoted a more even distribution of hMSCs throughout the scaffold and enhanced cell spreading at earlier time points without altering cell viability. This early induction of cell spreading and the uniform distribution of cells, in turn, may increase future proliferation and differentiation of hMSCs under conditions that simulate the microenvironment in vivo.

Ibuprofen loaded PLA nanofibrous scaffolds increase proliferation of human skin cells in vitro and promote healing of full thickness incision wounds in vivo

“…31 We used this facile approach to overcome the challenge of hydropobicity via atmospheric plasma treatment of the surfaces of the ibuprofen loaded PLA nanofibers. Evaluation of the hydrophilic properties of scaffolds using contact angle measurements, a commonly used method for surface hydrophilicity analyses 29,31,32 confirmed an increase in hydrophilicity of the scaffolds after plasma treatment with greatest hydrophilicity occurring on plasma treated 20% ibuprofen scaffolds. This increase in hydrophilicity likely improved adhesion of both HEK and HDF to the ibuprofen loaded PLA nanofibrous scaffolds.…”

Osteogenic Effects of Rest Inserted and Continuous Cyclic Tensile Strain on hASC Lines with Disparate Osteodifferentiation Capabilities

“…Human ASCs were isolated from excess adipose tissue remaining from either abdominoplasty or liposuction procedures performed at UNC Hospitals in accordance with an approved IRB protocol (IRB: 04-1622) as previously described. 5,18,31,63,64 Tissue samples were obtained from two female donors, ages 36 and 44. Cells were isolated and characterized as described in our previous studies.…”

“…1,20,32,59 Differentiation potential has been reported to vary substantially from donor to donor, 36,47,48,59 a phenomenon also experienced with cells isolated in our lab. 18 ASCs and MSCs may also require different culture medium formulations to differentiate down specific pathways. 3,14,15 These findings, along with differences in surface markers 9,68 and upregulation of different genes during differentiation, 27 all suggest that ASCs behave differently than MSCs from different source tissues.…”

The promising role for adipose-derived mesenchymal stem cells in tissue regeneration

“…One demonstrated technique to improve adhesion of ASCs to non-woven poly(L-lactic acid) scaffolds is oxygen plasma treatment prior to seeding [153]. Culture conditions, specifically the media / serum, also can have an effect on initial density of ASC seeding of scaffolds [154].…”