Effects of Oxygen Plasma Treatment on Parylene C and Parylene N Membrane Biocompatibility for Tissue Barrier Models

Abstract

Porous membranes are integral components of in vitro tissue barrier and co-culture models and their interaction with cells and tissues directly affects the performance and credibility of these models. Plasma-treated Parylene C and Parylene N are two biocompatible Parylene variants with perceived potential for use in these models but their biocompatibility and biological interactions at their interface with cells are not well understood. Here, we use a simple approach for benchtop oxygen plasma treatment and investigate the changes in cell spreading and extracellular matrix deposition as well as the changes in material surface properties. Our results support the previous findings on the persistent effects of plasma treatment on Parylene biocompatibility while showing a more pronounced improvement for Parylene C over Parylene N. It is observed that although both increased surface roughness and persistent increases in oxygen species govern the plasma-driven improvement, the changes in oxygen concentration at the interface are the dominant factor. Overall, the results of this study provide a clear picture of potential mechanisms of plasma-induced changes in synthetic polymers which have implications for their use in in vitro model systems and other BioMEMS applications.

Multifunctional Medical Grade Resin with Enhanced Mechanical and Antibacterial Properties: The Effect of Copper Nano-Inclusions in Vat Polymerization (VPP) Additive Manufacturing

“…Their biocompatibility, how to certify it, and the processing requirements have been presented in the literature [ 45 ]. Medical grade resins have been employed in cadavers for pelvic tumor resection [ 46 ], for bone fixation in femoral fractures in orthopedics [ 47 ], in surgical instruments [ 48 ], in face anatomy for the fabrication of face masks [ 49 ], in health monitoring devices [ 50 ], in tracheal grafts [ 51 ], in implantable vaccines [ 52 ], in tissues [ 53 ], in bio-microfluidic devices [ 54 ], in oncological orthopedics [ 55 ], in medical inhalers [ 56 ], and in eye treatments [ 57 ]. Nanocomposites have been developed to enhance the mechanical performance of medical-grade resins [ 58 ] and to induce antibacterial properties [ 59 ].…”

Parylene-C coating protects resin 3D printed devices from materials erosion and cytotoxicity

“…Recently, a few reports have shown that coating resin 3D printed materials with parylene-C increased their compatibility with cell lines in direct or indirect contact with the materials. [13][14][15][16] Parylene derivatives are a trusted material used for coating neural or cardiovascular implants and stents, 17 as well as a substrate for microscale cultures. 18,19 Parylene-C is the most affordable and commonly used derivative of the para-xylylene polymers and is conformally applied via vaporization.…”