Effects of oxygen concentration on embryonic development in rats: a light and electron microscopic study using whole-embryo culture techniques

Miki, Akinori; Fujimoto, Etsuko; Ohsaki, T.; Mizoguti, Humio

doi:10.1007/bf00698664

Cited by 42 publications

(13 citation statements)

References 16 publications

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“…Cultures were gassed 3 times, the first gas phase lasting 18 h (5% O 2 , 5% CO 2 and 90% N 2 ), the second phase until hour 26 (20% O 2 , 5% CO 2 and 75% N 2 ), and the third phase for the remainder of the 48-hour incubation period (40% O 2 , 5% CO 2 and 55% N 2 ). The progressive gas phase changes are necessary to avoid initial oxygen toxicity and allow optimal growth in later stages [New et al, 1976;Miki et al, 1988].…”

Section: Methodsmentioning

confidence: 99%

Effect of Matrix Metalloproteinase Inhibitors on Rat Embryo Development in vitro

Bigio

Seyoum²

1999

Cells Tissues Organs

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Matrix metalloproteinases appear to play a role in cell migration and tissue remodeling and are postulated to be important in the development of embryos. We hypothesized that inhibition of these proteinases with the synthetic inhibitor batimastat or with endogenous tissue inhibitor of metalloproteinases (TIMP) would alter in vitro development of day 9 rat embryos. Batimastat had a dose-related effect on embryo growth and viability following 48 h in culture, with significant inhibition at 1.0 μM concentration. Dying cells were observed in many organs of living embryos. TIMP-2 at 3.0 μg/ml had a similar effect on embryo growth. TIMP-1 alone or used in combination with TIMP-2 had no obvious effect. No focal malformations were observed. The effective concentrations are comparable to those which inhibit enzyme activity in cell culture. We conclude that matrix metalloproteinases are probably important in mammalian embryo development.

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Section: Methodsmentioning

confidence: 99%

Effect of Matrix Metalloproteinase Inhibitors on Rat Embryo Development in vitro

Bigio

Seyoum²

1999

Cells Tissues Organs

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“…Other factors may be related to the handling of the blastocysts before grafting. It is well known, for example, that successful cultivation of mammalian embryos depends on the correct oxygen concentration [Morriss and New, 1979;Miki et al, 1988]. Optimal implantation sites and oxygen concentrations will be explored in future studies.…”

Section: Discussionmentioning

confidence: 99%

Use of the Coelomic Grafting Technique for Prolonged ex utero Cultivation of Late Preprimitive Streak-Stage Rabbit Embryos

Püschel¹,

Männer²

2016

Cells Tissues Organs

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Due to its morphological similarity with the early human embryo, the pregastrulation-stage rabbit may represent an appropriate mammalian model for studying processes involved in early human development. The usability of mammalian embryos for experimental studies depends on the availability of whole embryo culture methods facilitating prolonged ex utero development. While currently used culture methods yield high success rates for embryos from primitive streak stages onward, the success rate of extended cultivation of preprimitive streak-stage mammalian embryos is low for all previously established methods and for all studied species. This limits the usability of preprimitive streak-stage rabbit embryos in experimental embryology. We have tested whether the extraembryonic coelom of 4-day-old chick embryos may be used for prolonged ex utero culture of preprimitive streak-stage rabbit embryos (stage 2, 6.2 days post coitum). We found that, within this environment, stage 2 rabbit blastocysts can be cultured at decreasing success rates (55% after 1 day, 35% after 2 days, 15% after 3 days) up to a maximum of 72 h. Grafted blastocysts can continue development from the onset of gastrulation to early organogenesis and thereby form all structures characterizing age-matched controls (e.g. neural tube, somites, beating heart). Compared to normal controls, successfully cultured embryos developed at a slower rate and finally showed some structural and gross morphological anomalies. The method presented here was originally developed for whole embryo culture of mouse embryos by Gluecksohn-Schoenheimer in 1941. It is a simple and inexpensive method that may represent a useful extension to presently available ex utero culture systems for rabbit embryos.

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“…Osmoregulatory disturbances and hypervolemia produced by moderate hypoxia resulted in abnormal development [9]. Miki et al [12] demonstrated that hypoxic conditions in cultured rat embryos caused retardation of embryonic growth and abnormal development, and the changes were due to cellular degeneration and necrosis in the neural tube, the somites and the mesenchyme. The yolk sac in culture is mainly responsible for the nutrition of cultured embryos [6,7].…”

Section: Discussionmentioning

confidence: 99%

A Calcium Agonist, Bay k 8644, Suppresses the Embryotoxic Effects Induced by Dihydropyridines Calcium Channel Blockers in Cultured Rat Embryos.

Ban

Makita

1998

The Journal of Veterinary Medical Science

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ABSTRACT. Day 9 rat embryos were exposed to 1,4-dihydropyridine calcium channel blockers; nifedipine (NIF), nicardipine (NIC) or nitrendipine (NIT), for 48 hr in the whole embryo culture system. There were dose-dependent growth retardation and abnormalities, predominantly in cardiovascular system. The three compounds exhibited very similar pattern of dysmorphogenic effects, but the potency of these compounds were quantitatively different. The incidences of embryos with the abnormalities were 100%, 100% and 85% following either exposure of NIF, NIC or NIT at concentration of 300, 8 and 15 µM, respectively. This study was to investigate whether these blocker-induced embryotoxicity was due to calcium channel blocking properties themselves in the embryos. Day 9 rat embryos were co-exposed to 1,4-dihydropyridine calcium channel agonist, Bay k 8644 (BAY) and each calcium channel blocker under the same culture condition. The retarded embryonic growth induced by 200 or 300 µM of NIF, 8 µM of NIC and 15 µM of NIT nearly or completely ameliorated when embryos were co-exposed with BAY at one-third or half concentration of each calcium channel blocker. Supplementation of BAY reduced the incidence of abnormalities by NIF-, NIC-and NIT-alone. These results suggested that one of mechanisms for embryotoxicity induced by calcium channel blocker was directly related to channel blocking property of the chemicals. -KEY WORDS: Bay k 8644, calcium channel blocker, culture, embryo, rat.J. Vet. Med. Sci. 60(10): 1067-1072, 1998 (NIT) were also investigated whether the observed embryotoxicities were basically class effects for dihydropyridines calcium channel blockers. The whole embryo culture system [13] was employed since the method allows observation of the direct effects of drug on the embryonic morphogenesis, and it also eliminates maternal factors as well as environmental influences [5]. Embryos were co-exposed to either NIF, NIC or NIT and BAY, and examined whether the embryotoxic effects of each calcium channel blocker are suppressed. BAY has positive inotropic and vasoconstrictor actions through the activation of voltagedependent calcium channel in contrast to the negative inotropic and vasodilator effects of NIF, even though BAY belongs to the dihydropyridines and is similar in structure to NIF [19,20]. MATERIALS AND METHODS Animals:Sprague-Dawley rats of the Crj: CD(SD) strain at approximately 11 weeks of age purchased from Charles River Japan Inc. were kept in an animal room where the temperature (22 ± 2°C), the relative humidity (55 ± 10%) and the light and dark cycle (12 hr each) were controlled. They were allowed to have free access to Purina Rodent Chow and tap water. Females were mated overnight with mature male breeders of the same strain. The day on which a vaginal plugs were found was designated as gestational day (GD) 0.Embryo culture: Females were euthanized by carbon dioxide asphyxiation on GD 9. The uterus was excised and all individual implantation sites were placed in a sterile dish containing Hank's balanced s...

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Effects of oxygen concentration on embryonic development in rats: a light and electron microscopic study using whole-embryo culture techniques

Cited by 42 publications

References 16 publications

Effect of Matrix Metalloproteinase Inhibitors on Rat Embryo Development in vitro

Effect of Matrix Metalloproteinase Inhibitors on Rat Embryo Development in vitro

Use of the Coelomic Grafting Technique for Prolonged ex utero Cultivation of Late Preprimitive Streak-Stage Rabbit Embryos

A Calcium Agonist, Bay k 8644, Suppresses the Embryotoxic Effects Induced by Dihydropyridines Calcium Channel Blockers in Cultured Rat Embryos.

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