Effects of oxidized corn oil and a synthetic antioxidant blend on performance, oxidative status of tissues, and fresh meat quality in finishing barrows

Boler, D. D.; Fernández-Dueñas, D. M.; Kutzler, L. W.; Zhao, Junmei; Harrell, R. J.; Campion, D. R.; McKeith, F. K.; Killefer, J.; Dilger, A. C.

doi:10.2527/jas.2012-5266

Cited by 65 publications

(107 citation statements)

References 32 publications

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“…The same result on G:F was observed. In disagreement with the present data, Boler et al (2012) reported that dietary supplementation with oxidized corn oil for 56 d in barrows reduced ADG and BW. This should be related to the different length of dietary supplementation and substance inducing oxidative stress.…”

Section: Discussioncontrasting

confidence: 99%

The effects of dietary verbascoside on blood and liver oxidative stress status induced by a high n-6 polyunsaturated fatty acids diet in piglets12

Giancamillo

Rossi

Pastorelli

et al. 2015

Journal of Animal Science

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Twenty-four weaned female Hypor piglets (10.9 ± 0.1 kg mean BW) were used to evaluate the antioxidant effect of a natural extract, titrated in verbascoside, on blood and liver oxidative status in relation to a high intake of n-6 PUFA, inducing oxidative stress. Piglets were assigned to 1 of 3 experimental groups; the first group was fed a diet with 9% sunflower oil (T1) and the second received the sunflower oil diet supplemented with 5 mg of verbascoside/kg feed from Verbenaceae extract (Lippia spp.; T2). The third group was fed a control diet (CTR), in which an isoenergetic replacement of oil by starch was done. Blood samples were collected at the beginning and the end of the trial (30 d). At the end of the trial, the animals were slaughtered and the liver specimens were collected. Oxidative stress markers, including total antiradical activity, superoxide dismutase (SOD), glutathione peroxidase (GPX), and catalase (CAT) activities, were determined in blood samples. Alanine aminotransferase (ALT), aspartate aminotransferase (AST), and γ-glutamyl transferase (GGT) plasma levels were also evaluated. Immunohistochemistry and western blot analyses were performed in liver to evaluate heat shock protein (Hsp) 70, Hsp90, and Kupffer and Ito cell activation. Liver activities of SOD, GPX, and CAT were also determined. Total antiradical activity in blood and red blood cells were affected (P < 0.01) by dietary treatments. The n-6 PUFA supplementation at a high dosage for 30 d induced oxidative stress, decreasing total antiradical activity in blood and red blood cells (CTR vs. T1 + T2; P < 0.01) and plasma CAT activity (CTR vs. T1 + T2; P = 0.088) and increasing ALT value (CTR vs. T1 + T2; P < 0.01). Also, in liver, the CAT and GPX activities tended to be lower in pigs fed n-6 PUFA diets than pigs fed a control diet (CTR vs. T1 + T2; = 0.090 and = 0.085, respectively). The liver samples presented a normal architecture and no Ito and Kupffer cell activations were observed. In liver, the SOD activity tended to be lower in the T1 group (P = 0.064) than in the CTR and T2 groups. Moreover, the level of Hsp70 was higher (P < 0.01) in the T1 group than the CTR and T2 groups. These data suggest that the dose of dietary verbascoside partially restores the antioxidant status of the liver without affecting the systemic responses to oxidative stress induced by a high-fat diet.

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Section: Discussioncontrasting

confidence: 99%

The effects of dietary verbascoside on blood and liver oxidative stress status induced by a high n-6 polyunsaturated fatty acids diet in piglets12

Giancamillo

Rossi

Pastorelli

et al. 2015

Journal of Animal Science

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“…Restoration to nearly normal concentrations of MDA by antioxidant blend may be due to an enhancement of the body's antioxidant defense system. This result is consistent with a previous swine study in which an elevation of TBARS was found after oxidized oil was applied, whereas AOX reduced plasma and muscle TBARS (Boler et al, 2012).…”

Section: Discussionsupporting

confidence: 93%

Supplementing antioxidants to pigs fed diets high in oxidants: I. Effects on growth performance, liver function, and oxidative status1

Harper

Zhao³

et al. 2014

Journal of Animal Science

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The objective of the study was to determine the effects of a dietary antioxidant blend (ethoxyquin and propyl gallate) and vitamin E on growth performance, liver function, and oxidative status in pigs fed diets high in oxidants. Crossbred barrows (n=100, 10.91±0.65 kg BW, 36±2 d of age, Landrace×Duroc) were allotted to 5 treatments on the basis of BW (5 replicate pens per treatment, 4 pigs per pen). Treatments included 1) HO, high-oxidant diet containing 5% oxidized soybean oil and 10% PUFA source (providing 2.05% docosahexaenoic acid in the diet), 2) VE, the HO diet with 11 IU/kg of added vitamin E, 3) AOX, the HO diet with antioxidant blend (135 mg/kg), 4) VE+AOX, the HO diet with both vitamin E and antioxidant blend, and 5) SC, a standard corn-soy control diet. The trial lasted for 118 d; on d 83, the HO diet pigs were switched to the SC diet because the animals were displaying very poor health. Compared with SC pigs, HO pigs had decreased ADG (0.92 vs. 0.51 kg for d 26 to 55, 1.29 vs. 0.34 kg for d 56 to 82; P<0.05) and ADFI (1.84 vs. 0.96 kg for d 26 to 55, 3.41 vs. 1.14 kg for d 56 to 82; P<0.05). However, switching the HO pigs to the SC diet resulted in HO pigs having a greater ADG than VE-fed pigs from d 83 to 118 (0.90 vs. 0.60 kg; P<0.05). The antioxidant blend restored pig performance to a level similar that of pigs fed the SC diet (P>0.05) with greater G:F for the entire period (0.44 vs. 0.38; P<0.05). A greater liver to BW ratio was found in HO compared with other treatments on d 55 and in VE on d 118. Total bilirubin concentration in plasma of HO pigs on d 55 was greater than that in VE+AOX pigs (P<0.05), whereas on d 118, bilirubin concentration in VE was higher than those in VE+AOX and SC (P<0.05). A similar trend was observed in aspartate transaminase. Plasma concentrations of thiobarbituric acid reactive substances (TBARS) and carbonyl were elevated (P<0.05) in the HO pigs compared with the SC pigs on d 55 but not on d 118. Liver TBARS and carbonyl concentrations showed a similar trend, except that HO pigs had the greatest carbonyl concentration on d 118. Pigs fed AOX diets had plasma and liver TBARS and carbonyl concentrations similar to those fed SC diets. In the oxidative stress model used in this study, dietary addition of antioxidant blend or antioxidant blend+vitaimin E was effective in improving growth, liver function, and plasma markers of oxidative stress, but VE alone was not.

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“…Birds were fed a common commercial-type diet until day 8. Oxidized oil induced oxidative stress in animals [22], which can then cause tendon damage [20]. To exacerbate the stress to tendon, birds in one pen ( Figure 1B) were fed oxidized fat diet that included 5% oxidized soybean oil starting on day 8.…”

Section: Bird Trials and Tissue Sample Preparationmentioning

confidence: 99%

“…Oxidative stress has been reported to exacerbate joint disease in a rat arthritis model [20] and associate with cartilage degeneration in an experimental canine model of osteoarthritis [21]. Dietary oxidized oil has been reported to induce oxidative stress in animals [22].…”

Section: Introductionmentioning

confidence: 99%

Quantitative Analysis of Tendon Structural Integrity in Chickens using Fourier Transform-Second Harmonic Generation Imaging

Sivaguru¹

2014

Cell Dev Biol

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Degenerative changes in tendons are common in human and land-dwelling vertebrates of all species, and are associated with both age and use. Tendon rupture and inflammation are common causes of lameness in broilers and breeders at multiple ages and has become a very important issue in the poultry industry attempts to analyze tendon health have relied primarily on visual assessment. The ability to conduct meaningful experiments on causes and interventions would be much improved by a direct assay of tendon structural integrity. The objective of this study is to test whether we can use Fourier transform-second harmonic generation (FT-SHG) imaging technique to quantify the differences in collagen fiber organization and nuclei morphology between normal and injured chicken tendons. Tendon injury/degeneration was induced by rearing chickens in wire-floor pens and feeding them an oxidized fat diet. Injured tendons had greater collagen fiber degradation/degeneration; more randomly organized collagen fibers, higher cellularity and more circular-shaped nuclei than normal tendons, indicating loss of collagen tertiary structure and lateral compression in injured tendons. Therefore, analysis of collagen fiber organization and nuclear morphology using FT-SHG imaging is a potential diagnostic tool to evaluate tendon structural health and to test the effects of nutrients and intervention strategies on the structural development of tendons in chickens.

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Effects of oxidized corn oil and a synthetic antioxidant blend on performance, oxidative status of tissues, and fresh meat quality in finishing barrows

Cited by 65 publications

References 32 publications

The effects of dietary verbascoside on blood and liver oxidative stress status induced by a high n-6 polyunsaturated fatty acids diet in piglets12

The effects of dietary verbascoside on blood and liver oxidative stress status induced by a high n-6 polyunsaturated fatty acids diet in piglets12

Supplementing antioxidants to pigs fed diets high in oxidants: I. Effects on growth performance, liver function, and oxidative status1

Quantitative Analysis of Tendon Structural Integrity in Chickens using Fourier Transform-Second Harmonic Generation Imaging

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