Effects of oxidative stress on the virulence profile of Prevotella intermedia during experimental infection in gnotobiotic mice

Santos, Simone Gonçalves dos; Diniz, Cláudio Galuppo; Silva, Vânia L.; Martins, W.A.; Cara, Denise Carmona; Souza, Natália Cristina de; Serufo, José Carlos; Nicoli, Jacques R.; Carvalho, Maria Auxiliadora Roque de; Farias, Luiz M.

doi:10.1099/jmm.0.46748-0

Cited by 6 publications

(3 citation statements)

References 23 publications

(23 reference statements)

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“…Because of its central bridging role and protective effect against ROS within the oral biofilm, it is crucial to understand the biochemical and physiological mechanisms utilized by FNN to survive oxidative stress. This response to oxidative stress likely contributes to the organism's virulence 24, 25. Although a proteomic analysis of endodontic specimens containing FNN from root canal infections has been reported 26, as well as proteomic studies addressing the effect of pH 27, 28 and ampicillin 29 on FNN , these studies have annotated only a limited part of the FNN proteome.…”

Section: Introductionmentioning

confidence: 99%

Oxidative stress response in the opportunistic oral pathogen Fusobacterium nucleatum

et al. 2011

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The anaerobic, Gram-negative bacillus Fusobacterium nucleatum plays a vital role in oral biofilm formation and the development of periodontal disease. The organism plays a central bridging role between early and late colonizers within dental plaque and plays a protective role against reactive oxygen species. Using a two-dimensional gel electrophoresis and mass spectrometry approach, we have annotated 78 proteins within the proteome of F. nucleatum subsp. nucleatum and identified those proteins whose apparent intracellular concentrations change in response to either O 2 -or H 2 O 2 -induced oxidative stress. Three major protein systems were altered in response to oxidative stress: (i) proteins of the alkyl hydroperoxide reductase/thioredoxin reductase system were increased in intracellular concentration; (ii) glycolytic enzymes were modified by oxidation (i.e. D-glyceraldehyde 3-phosphate dehydrogenase, and fructose 6-phosphate aldolase) or increased in intracellular concentration, with an accompanying decrease in ATP production; and (iii) the intracellular concentrations of molecular chaperone proteins and related proteins (i.e. ClpB, DnaK, HtpG, and HrcA) were increased.

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Section: Introductionmentioning

confidence: 99%

Oxidative stress response in the opportunistic oral pathogen Fusobacterium nucleatum

et al. 2011

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“…Both are associated with periodontal diseases [41]. They initially bind to organisms enhancing the adherence of other microbes to the oral cavity [42,43]. Therefore, they hold a central role in the onset Table 4: Effect of the 3 least toxic products to fibroblast viability.…”

Section: Discussionmentioning

confidence: 99%

Comparison of Antimicrobial and Wound Healing Agents on Oral Fibroblast Viability and In-vivo Bacterial Load

C¹

2015

Dentistry

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With links to so many serious diseases, the importance of controlling oral bacteria is clear. The dental intervention for periodontal disease typically includes mechanical removal of the plaque biofilm and/ or surgical procedures in advanced cases. Scaling and root planning are currently the gold standard of non-surgical care for chronic periodontitis. In addition, the application of antimicrobials has been shown to be effective in improving probe depth measurements, and clinical attachment levels compared to mechanical cleaning alone [20]. Chlorhexidine (CHX) is commonly used as a major component in mouth rinses to reduce gingival infections. At high concentrations, it acts by damaging the bacterial cell wall, leading to bacterial death [21]. While there are studies indicating beneficial reductions of oral bacterial in response to CHX treatment [22], there are reports that rinsing with CHX has little or no long-term impact on oral health [20,23]. Other antimicrobial rinses include carbamide peroxide, which at high enough concentrations elevates the mouth pH to levels that are harmful to bacteria [24]. However, it can also cause a reduction in hardness of some of the tooth enamel areas, and is best known as a teeth-whitening treatment rather than as an antimicrobial agent [25]. Other commercially-available antimicrobial oral rinses are based on essential oils like eucalyptol, menthol, and thymol. Like studies on the other oral rinses, the results of studies on the antibacterial effects of essential oil products have also been mixed [26-28].

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“…The oxidative stress tolerance was evaluated by the disk-diffusion method, according to that previously described [15]. In brief, paper disks of 5 mm diameter were soaked with 5µl of 20% hydrogen peroxide and placed on Mueller Hinton plates (BD-Difco, USA) previously inoculated with 0.5 McFarland bacterial suspensions.…”

Section: Evaluation Of K Pneumoniae Physiological Characteristics (Omentioning

confidence: 99%

Physiological and molecular characteristics of carbapenem resistance in Klebsiella pneumoniae and Enterobacter aerogenes

Silva

Dias

Ferreira-Machado

et al. 2016

J Infect Dev Ctries

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Introduction: Bacterial resistance is a growing concern in the nosocomial environment in which Klebsiella pneumoniae and Enterobacter aerogenes play an important role due to their opportunism and carbapenemase-production. This work aimed to evaluate physiological and molecular characteristics of carbapenem-resistant K. pneumoniae and E. aerogenes isolated in a Brazilian tertiary hospital. Methodology: In total, 42 carbapenem-resistant bacteria isolated from clinical specimens were included (21 K. pneumoniae and 21 E. aerogenes). Drug-sensitive K. pneumoniae (n = 27) were also included. Antimicrobial susceptibility and biocide tolerance patterns, hemolytic activity, tolerance to oxidative stress, and aggregative ability were assessed. Genetic markers related to carbapenem resistance, or ESBLproduction were screened by PCR. Results: Compared to drug-sensitive strains, carbapenem-resistant K. pneumoniae were more tolerant to biocides and to oxidative stress, and they displayed an increase in biofilm formation. The genetic markers blaKPC (95.2%) and blaTEM (90.5%) were the most frequent. Among the carbapenem-resistant E. aerogenes strains, blaKPC, and blaTEM were detected in all bacteria. Drug-sensitive E. aerogenes were not isolated in the same period. blaSHV, blaVIM, and blaCTX markers were also observed among carbapenem-resistant bacteria. Conclusions: Results suggest that carbapenemase-producing enterobacteria might show peculiar characteristics regarding their physiology associated with their environmental persistency, virulence, and multidrug resistance. The observed phenomenon may have implications not only for antimicrobial chemotherapy, but also for the prognosis of infectious diseases and infection control.

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Effects of oxidative stress on the virulence profile of Prevotella intermedia during experimental infection in gnotobiotic mice

Cited by 6 publications

References 23 publications

Oxidative stress response in the opportunistic oral pathogen Fusobacterium nucleatum

Oxidative stress response in the opportunistic oral pathogen Fusobacterium nucleatum

Comparison of Antimicrobial and Wound Healing Agents on Oral Fibroblast Viability and In-vivo Bacterial Load

Physiological and molecular characteristics of carbapenem resistance in Klebsiella pneumoniae and Enterobacter aerogenes

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