Effects of oxidative stress induced by environmental relevant concentrations of fluoxetine on the embryonic development on Danio rerio

Orozco-Hernández, José Manuel; Gómez-Oliván, Leobardo Manuel; Elizalde-Velázquez, Gustavo Axel; Heredia-García, Gerardo; Cardoso-Vera, Jesús Daniel; Dublán‐García, Octavio; Islas-Flores, Hariz; SanJuan-Reyes, Nely; Galár-Martínez, Marcela

doi:10.1016/j.scitotenv.2021.151048

Cited by 22 publications

(8 citation statements)

References 61 publications

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“…To simulate environmentally realistic pollution scenarios, according to previous studies, ,, 0 (control), 30 (to mimic the common pollution level of FLX in surface water), and 300 ng L –1 (to mimic heavy FLX pollution in water adjacent to sewage outlets) were adopted as the exposure concentrations of FLX in this study. In brief, following the methods reported previously, , after acclimation, 270 fish individuals were randomly assigned to 9 experimental tanks (3 exposure groups × 3 replicates, 30 fish/replicate) filled with 30 L of aerated tap water containing corresponding designed doses of FLX.…”

Section: Methodsmentioning

confidence: 99%

“…However, growing evidence indicates that exposure to waterborne FLX may put this ecologically and commercially important group of animals in great danger by altering the in vivo level of 5-HT and subsequently disturbing downstream 5-HT-related physiological processes . According to previous studies, the metabolism, embryonic development, growth, and behavioral response of different fish species could be significantly interrupted by FLX exposure. For instance, anomalies in behaviors related to locomotion, sociability, anxiety, and antipredator response have been observed in FLX-exposed fish .…”

Section: Introductionmentioning

confidence: 99%

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Anti-Depressant Fluoxetine Hampers Olfaction of Goldfish by Interfering with the Initiation, Transmission, and Processing of Olfactory Signals

Huang

Zhang

Han

et al. 2022

Environ. Sci. Technol.

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The ubiquitous presence of fluoxetine (FLX) in aquatic environments poses great threat to fish species. However, little is known about its deleterious impacts on fish olfaction. In this study, the olfactory toxicity of FLX at environmentally realistic levels was assessed by monitoring the behavioral and electroolfactogram (EOG) responses to olfactory stimuli with goldfish (Carassius auratus), and the toxification mechanisms underlying the observed olfaction dysfunction were also investigated. Our results showed that the behavioral and EOG responses of goldfish to olfactory stimuli were significantly weakened by FLX, indicating an evident toxicity of FLX to olfaction. Moreover, FLX exposure led to significant alterations in olfactory initiation-related genes, suppression of ion pumps (Ca 2+ -ATPase and Na + /K + -ATPase), tissue lesions, and fewer olfactory sensory neurons in olfactory epithelium. In addition to altering the expression of olfactory transmission-related genes, comparative metabolomic analysis found that olfaction-related neurotransmitters (i.e., L-glutamate and acetylcholine) and the olfactory transduction pathway were significantly affected by FLX. Furthermore, evident tissue lesions, aggravated lipid peroxidation and apoptosis, and less neuropeptide Y were observed in the olfactory bulbs of FLX-exposed goldfish. Our findings indicate that FLX may hamper goldfish olfaction by interfering with the initiation, transmission, and processing of olfactory signals.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Anti-Depressant Fluoxetine Hampers Olfaction of Goldfish by Interfering with the Initiation, Transmission, and Processing of Olfactory Signals

Huang

Zhang

Han

et al. 2022

Environ. Sci. Technol.

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“…According to Oakes et al (2010), the PNEC SW (predicted no effect concentration for surface waters) value for fluoxetine is 0.012 μg/L. Throughout this review we have some articles where the concentrations tested are below this value (Al Shuraiqi et al, 2021;Farias et al, 2020;Orozco-Hernández et al, 2022a;Orozco-Hernández et al, 2022b;Saaristo et al, 2017;Zindler et al, 2020), producing significant effects at different endpoints (behavior, tissue structure and oxidative stress). These data show that fluoxetine can act at very low concentrations on specific targets that are scarcely addressed in conventional toxicity testing.…”

Section: Final Considerations and Future Perspectivesmentioning

confidence: 99%

Effects of fluoxetine on fish: What do we know and where should we focus our efforts in the future?

Correia

Domingues

Faria

et al. 2023

Science of The Total Environment

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“…Following exposure to FLX 0.012TU and FLX 0.0015TU for 96-h and 21 days in D. magna in vivo, the GPX activity was increased (Över et al 2020 ). Similarly, Orozco-Hernández et al ( 2022 ) found that 96-h exposure increased lipid peroxidation levels, and SOD, CAT, and GPX activities in Danio rerio embryos at concentrations between 15 and 40 ng/L. Therefore, while evaluating the effects of FLX alone on GPX activity, it could be required to take the concentration-dependence into account.…”

Section: Discussionmentioning

confidence: 99%

Binary effects of fluoxetine and zinc on the biomarker responses of the non-target model organism Daphnia magna

Atli,

Sevgiler

2024

Environ Sci Pollut Res

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The antidepressant effect of zinc on mammals has been documented in recent decades, and the concentration of the antidepressant fluoxetine (FLX) in aquatic environments has been rising constantly. The aim of the present study is to evaluate the combined toxicity of a serotonin reuptake inhibitor (FLX) and Zn2+ on a non-target aquatic model organism Daphnia magna. Animals were exposed to single and binary combinations of FLX (20.5 and 41 µg/L for subchronic and 41 and 82 µg/L for acute exposures) and Zn2+ (40 µg/L for subchronic and 80 µg/L for acute exposures). In vivo experiments were done for 7 days subchronic and 48 h acute exposure, while subcellular supernatants of whole Daphnia lysate (WDL) were directly treated with the same concentrations used in the acute experiments. Morphological characteristics, Ca2+-ATPase, antioxidant enzyme activities, and lipid peroxidation were examined. There was antioxidant system suppression and Ca2+-ATPase inhibition despite the diverse response patterns due to duration, concentration, and toxicant type. After acute exposure, biomarkers showed a diminishing trend compared to subchronic exposure. According to integrated biomarker response index (IBR) analysis, in vivo Zn2+ exposure was reasonably effective on the health of D. magna, whereas exposure of WDL to Zn2+ had a lesser impact. FLX toxicity increased in a concentration-dependent manner, reversed by the combined exposure. We concluded that potential pro-oxidative and adverse Ca2+-ATPase effects of FLX and Zn2+ in D. magna may also have harmful impact on ecosystem levels. Pharmaceutical exposure (FLX) should be considered along with their potential to interact with other toxicants in aquatic biota. Graphical Abstract

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Effects of oxidative stress induced by environmental relevant concentrations of fluoxetine on the embryonic development on Danio rerio

Cited by 22 publications

References 61 publications

Anti-Depressant Fluoxetine Hampers Olfaction of Goldfish by Interfering with the Initiation, Transmission, and Processing of Olfactory Signals

Anti-Depressant Fluoxetine Hampers Olfaction of Goldfish by Interfering with the Initiation, Transmission, and Processing of Olfactory Signals

Effects of fluoxetine on fish: What do we know and where should we focus our efforts in the future?

Binary effects of fluoxetine and zinc on the biomarker responses of the non-target model organism Daphnia magna

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