Effects of overexpression of antioxidants on the release of cytochrome c and apoptosis-inducing factor in the model of ischemia

Zemlyak, Ilona; Brooke, Sheila M.; Singh, Maneesh H.; Sapolsky, Robert M.

doi:10.1016/j.neulet.2009.02.020

Cited by 18 publications

(17 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Over-expression of superoxide dismutase (SOD), both mitochondrial (MnSOD) and cytosolic (CuZnSOD), increases oxidative stress resistance [85], [86], [87]. In addition, over-expression of SODs, glutathione peroxidase (GPx) and catalase (CAT) also act to protect against ischemia associated cytochrome c release from mitochondria, thereby limiting the occurrence of apoptotic cell death [88]. Similarly, Page et al [89] found that most of the intracellular antioxidant enzymes, including the MnSOD, CuZnSOD, CAT, GPx and glutathione reductase were up-regulated in brain tissue of lungfish that had aestivated for 60 days.…”

Section: Discussionmentioning

confidence: 99%

Differential Gene Expression in the Brain of the African Lungfish, Protopterus annectens, after Six Days or Six Months of Aestivation in Air

Hiong

Wong

et al. 2013

PLoS ONE

View full text Add to dashboard Cite

The African lungfish, Protopterus annectens, can undergo aestivation during drought. Aestivation has three phases: induction, maintenance and arousal. The objective of this study was to examine the differential gene expression in the brain of P. annectens during the induction (6 days) and maintenance (6 months) phases of aestivation as compared with the freshwater control using suppression subtractive hybridization. During the induction phase of aestivation, the mRNA expression of prolactin (prl) and growth hormone were up-regulated in the brain of P. annectens, which indicate for the first time the possible induction role of these two hormones in aestivation. Also, the up-regulation of mRNA expression of tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein γ polypeptide and the down-regulation of phosphatidylethanolamine binding protein, suggest that there could be a reduction in biological and neuronal activities in the brain. The mRNA expression of cold inducible RNA-binding protein and glucose regulated protein 58 were also up-regulated in the brain, probably to enhance their cytoprotective effects. Furthermore, the down-regulation of prothymosin α expression suggests that there could be a suppression of transcription and cell proliferation in preparation for the maintenance phase. In general, the induction phase appeared to be characterized by reduction in glycolytic capacity and metabolic activity, suppression of protein synthesis and degradation, and an increase in defense against ammonia toxicity. In contrast, there was a down-regulation in the mRNA expression of prl in the brain of P. annectens during the maintenance phase of aestivation. In addition, there could be an increase in oxidative defense capacity, and up-regulation of transcription, translation, and glycolytic capacities in preparation for arousal. Overall, our results signify the importance of reconstruction of protein structures and regulation of energy expenditure during the induction phase, and the needs to suppress protein degradation and conserve metabolic fuel stores during the maintenance phase of aestivation.

show abstract

Section: Discussionmentioning

confidence: 99%

Differential Gene Expression in the Brain of the African Lungfish, Protopterus annectens, after Six Days or Six Months of Aestivation in Air

Hiong

Wong

et al. 2013

PLoS ONE

View full text Add to dashboard Cite

show abstract

“…We found that most of the intracellular antioxidant enzyme levels and/ or activities were indeed signiWcantly upregulated in brain and, to a lesser extent heart tissue. These increases in antioxidant activities would be expected to greatly enhance the cellular stress resistance of estivating lungWsh, based on studies in which the overexpression of individual antioxidant enzymes has conferred substantial protection against various physiological stressors (see, for example, Murakami et al 1997;Solaini and Harris 2005;Shan et al 2007;Zemlyak et al 2009). This would be expected to make P. dolloi more tolerant of stresses associated with estivation, such as transient hypoxia, hyperthermia, and dehydration.…”

Section: Discussionmentioning

confidence: 99%

Upregulation of intracellular antioxidant enzymes in brain and heart during estivation in the African lungfish Protopterus dolloi

Page¹,

Salway²,

et al. 2009

J Comp Physiol B

View full text Add to dashboard Cite

The African slender lungfish, Protopterus dolloi, is highly adapted to withstand periods of drought by secreting a mucous cocoon and estivating for periods of months to years. Estivation is similar to the diapause and hibernation of other animal species in that it is characterized by negligible activity and a profoundly depressed metabolic rate. As is typically observed in quiescent states, estivating P. dolloi are resistant to environmental stresses. We tested the hypothesis that P. dolloi enhances stress resistance during estivation by upregulating intracellular antioxidant defences in brain and heart tissues. We found that most of the major intracellular antioxidant enzymes, including the mitochondrial superoxide dismutase, cytosolic superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase, were upregulated in brain tissue of lungfish that had estivated for 60 days. Several of these enzymes were also elevated in heart tissue of estivators. These changes wer... Abstract The African slender lungWsh, Protopterus dolloi, is highly adapted to withstand periods of drought by secreting a mucous cocoon and estivating for periods of months to years. Estivation is similar to the diapause and hibernation of other animal species in that it is characterized by negligible activity and a profoundly depressed metabolic rate. As is typically observed in quiescent states, estivating P. dolloi are resistant to environmental stresses. We tested the hypothesis that P. dolloi enhances stress resistance during estivation by upregulating intracellular antioxidant defences in brain and heart tissues. We found that most of the major intracellular antioxidant enzymes, including the mitochondrial superoxide dismutase, cytosolic superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase, were upregulated in brain tissue of lungWsh that had estivated for 60 days. Several of these enzymes were also elevated in heart tissue of estivators. These changes were not due to food deprivation, as they did not occur in a group of Wsh that were deprived of food but maintained in water for the same period of time. We found little evidence of tissue oxidative damage in estivators. Products of lipid peroxidation (4-hydroxynonenal adducts) and oxidative protein damage (carbonylation) were similar in estivating and control lungWsh. However, protein nitrotyrosine levels were elevated in brain tissue of estivators. Taken together, these data indicate that estivating P. dolloi have enhanced oxidative stress resistance in brain and heart due to a signiWcant upregulation of intracellular antioxidant capacity.

show abstract

“…In fact, CAT overexpression in the heart of transgenic mice has been shown to provide myocardial protection against IR injury (Mele et al ., 2006). Additional strategies based on exogenously administered CAT enzyme (Forsman et al ., 1988; Castillo et al ., 1990) or on CAT overexpression by viral vector have been used to examine its protective role in IR injury both in in vitro and in vivo systems (Wang et al ., 2003; Gu et al ., 2004; Gáspár et al ., 2009; Kim et al ., 2009; Zemlyak et al ., 2009; Ushitora et al ., 2010; Chen and Tang, 2011). Of note, systemic infusion of CAT failed to improve neurological deficits after complete ischaemia (Forsman et al ., 1988), but resulted in a decrease of myocardial injury following coronary ischaemia (Gardner et al ., 1983).…”

Section: Can the Pharmacological Modulation Of Enzymatic Pathways Leamentioning

confidence: 99%

Therapeutic potential of targeting hydrogen peroxide metabolism in the treatment of brain ischaemia

Armogida

Nisticò

Mercuri

2012

British J Pharmacology

View full text Add to dashboard Cite

For many years after its discovery, hydrogen peroxide (H2O2) was viewed as a toxic molecule to human tissues; however, in light of recent findings, it is being recognized as an ubiquitous endogenous molecule of life as its biological role has been better elucidated. Indeed, increasing evidence suggests that H2O2 may act as a second messenger with a pro-survival role in several physiological processes. In addition, our group has recently demonstrated neuroprotective effects of H2O2 on in vitro and in vivo ischaemic models through a catalase (CAT) enzyme-mediated mechanism. Therefore, the present review summarizes experimental data supporting a neuroprotective potential of H2O2 in ischaemic stroke that has been principally achieved by means of pharmacological and genetic strategies that modify either the activity or the expression of the superoxide dismutase (SOD), glutathione peroxidase (GPx) and CAT enzymes, which are key regulators of H2O2 metabolism. It also critically discusses a translational impact concerning the role played by H2O2 in ischaemic stroke. Based on these data, we hope that further research will be done in order to better understand the mechanisms underlying H2O2 functions and to promote successful H2O2 signalling based therapy in ischaemic stroke. Abbreviations3-AT, 3-amino-1,2,4-triazole; ACSF, artificial cerebral spinal fluid; BSO, buthionine sulfoximine; CAT, catalase; DA, dopamine; DHE, dihydroethidium; fEPSP, field excitatory postsynaptic potential; GPx, glutathione peroxidase; HIF, hypoxia-inducible factor; IPC, ischaemic preconditioning; IR, ischaemia-reperfusion; KATP, ATP-sensitive K + channel; MCAo, middle cerebral artery occlusion; MCS, mercaptosuccinate; mTOR, mammalian target of rapamycin; NF, nuclear factor; NOS, nitric oxide synthase; O2, molecular oxygen; · O2 -, superoxide anion; OGD, oxygen/glucose-deprivation; •OH, hydroxyl radical; PGC1a, PPARg coactivator1a; PI3K, phosphatidylinositol 3-kinase; PPARg, peroxisome proliferator-activated receptorg; Prx, peroxiredoxin; ROS, reactive oxygen species; SNc, substantia nigra pars compacta; SOD, superoxide dismutase; TDP, thiolate-dependent phosphatase; Tg(CAT), transgenic mouse over-expressing catalase; TRP, transient receptor potential; WT, wild-type NomenclatureThe drug/molecular target nomenclature used in this review conforms to the British Journal of Pharmacology's Guide to Receptors and Channels (Alexander et al., 2011), where applicable. Historical notesThe history of H2O2 began in 1818 when it was discovered by Thénard who named it eau oxygénée (Thénard, 1818). Since the mid-1800s, H2O2 has been marketed for a wide variety of uses, including non-polluting bleaching, oxidizing agent, BJP British Journal of Pharmacology DOI:10.1111DOI:10. /j.1476DOI:10. -5381.2012 (Wolffenstein, 1894). In 1888, the first medical use of H2O2 was described by Love as efficacious in treating numerous diseases, including scarlet fever, diphtheria, nasal catarrh, acute coryza, whooping cough, asthma hay fever and tonsillitis (Love, 1888). Similar...

show abstract

Effects of overexpression of antioxidants on the release of cytochrome c and apoptosis-inducing factor in the model of ischemia

Cited by 18 publications

References 23 publications

Differential Gene Expression in the Brain of the African Lungfish, Protopterus annectens, after Six Days or Six Months of Aestivation in Air

Differential Gene Expression in the Brain of the African Lungfish, Protopterus annectens, after Six Days or Six Months of Aestivation in Air

Upregulation of intracellular antioxidant enzymes in brain and heart during estivation in the African lungfish Protopterus dolloi

Therapeutic potential of targeting hydrogen peroxide metabolism in the treatment of brain ischaemia

Contact Info

Product

Resources

About